Anaerobic Cultivation - Microbiology - Lecture Slides, Slides for Microbiology. Baddi University of Emerging Sciences and Technologies

Microbiology

Description: Anaerobic Cultivation, Anaerobic Infections, Anaerobic Environment, Anaerobic Culture Media, Sampling and Transport, Bacteria in Relation, Obligate Anaerobic, Microaerophilic are the important key points of lecture slides of Microbiology.
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Practical 7 ST
anaerobic cultivation
Bacteria in relation to oxygen
Anaerobic infections - sampling and transport
Cultivation
- establishing of anaerobic environment
- anaerobic culture media,
- procedure: inoculation, isolation
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Bacteria in relation to oxygen
Obligate aerobic -require gaseous oxygen, cannot grow
without oxygen
Obligate anaerobic - oxygen is toxic for them.
Methabolic pathway used for gaining energy is
fermentatio with production of foul-smelling end
products.
Facultative anaerobic - able to adapt to aerobic or
anaerobic
Microaerophilic - require reduced oxygen tension
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Anaerobic infections
Usually endogenous bacteria - disturbance of the
balance of normal flora (ATB and colon microflora) or
unusual anatomic sites (perforation of colon -
anaerobic peritonitis)
Exogenous bacteria - wound contamination
Exudate, pus, abscess
Sampling and transport in anaerobic conditions -
aspirate in the syringe with the needle capped or
protected with rubber. Surgical sampling can be
required. Never use the dry swab!
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Establishing of anaerobic conditions
Candle jar - microaerophilic, facultative anaerobic
Anaerobic jar with atmosphere generator (foil envelopes): release hydrogen
and carbon dioxide + catalyser (should be regenerated at regular interval)
after addition of water. Anaerobiosis redox indicator strips
Anaerostat - bottle with anaerobic athomosphere
Biological method - cocultivation with Serratia marcescens
Tubes of broth cultures (thioglycolate, cooked meat) - their formulations
contain reducing substances - anaerobic conditions at the lower part - fresh
and screwed well - regeneration - 15 minutes in boiling water bathwith
screw loosened to dissolve oxygen, screx tightened and cool
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