Pulling apart DNA and RNA, Exams of Genetics

Download Pulling apart DNA and RNA and more Exams Genetics in PDF only on Docsity! Pulling apart DNA and RNA Paul Grayson December 1, 2001 1 Introduction Measurements of individual molecules are necessary for an understanding of many biological processes. The properties of DNA and RNA strands are particularly important, since these molecules are central to the func- tion of all organisms. In the past five years, several experiments have be- gun to measure the forces holding these molecules together — by pulling them apart, using optical tweezers, piezoelectric actuators, or atomic force microscopy. These experiments demonstrate various properties of single- stranded DNA [3], single-stranded RNA [2], and double-stranded DNA [1, 3]. As it improves, the technique will be used more and more for its ability to quickly reveal information about specific DNA and RNA sequences. 2 Features of double-stranded DNA As discussed in class, Bockelmann et al [1] performed an experiment in which the two strands of a large double-helix of DNA were repeatedly pulled apart and allowed to join together again. One strand is connected to a movable microscope slide, and the other to the end of a flexible lever, and a precise measurement of the deflection of the lever allows the tension applied to the DNA to be determined. The measurements agree very well with a simple the- oretical calculation similar to the one described in class: if the local densities of G-C and A-T pairs is known, the force profile can be roughly predicted. This analysis is based on two assumptions: 1. There are just two relevant binding energies: EA-T and EG-C — in- teractions of neighboring strands are unimportant. 1 2. The total free energy in the system can be analyzed as a sum of indepen- dent contributions: the base pair binding energy, the single-stranded DNA coiling energy, and the lever deflection energy. There must be no significant interactions between these separate parts. The experiments on single-stranded DNA by Rief et al [3] (described in Section 3) reveal a little more about the validity of these assumptions. Before discussing their results in this area, however, we will introduce the first part of their experiment, which is a completely different way of melting the two strands of a DNA double-helix. They attached both strands of one end of the helix to a substrate, and pulled on one strand of the other end with the tip of an atomic force microscope. This one strand gradually straightened out under tension, stretching the bonds between it and the second, unattached strand. A sharp transition was seen at force of 30–70pN, interpreted as the breaking of individual H-bonds holding the two strands together. Beyond this transition, the second strand does not, however, detach from the first: it is assumed to relax into a less-stretched helix where it can again form H- bonds with the first strand. At forces of 150-350pN, another phase transition is usually observed: this is interpreted as the melting of the double-helix. The most important thing shown by this different method of pulling on double-stranded DNA is that the forces required for both transitions are sequence dependent. The wide range of critical forces mentioned above cor- responds to the difference between GCGCGC CGCGCG and ATATAT TATATA DNA, respec- tively the most strongly and most weakly bound DNA used in the experiment. Since the strands do not unzip gradually, however, the required forces can probably only be compared with the average concentrations of the two types of base pairs across the whole segment of DNA. The critical forces will be mostly independent of the order of the G-C and A-T pairs. 3 Single-stranded RNA and DNA Single-stranded DNA can also form helices, by binding to itself like RNA. In the study by Rief et al, one strand of a double-stranded GCGCGC CGCGCG or ATATAT TATATA DNA molecule was stretched until the tension induced on the other strand was sufficient to partially melt the double-helix. Because of the self-complementarity of its sequence, the free strand then bound to itself, folding into a helical hairpin structure like that of RNA. Releasing the tension 2