ALAT Certification Study Guide: Questions & Answers for Technicians, Exams of Laboratory Practices and Management

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ALAT Certification Study Questions and

Answers latest update 2023

Other than the ALAT Training Manual, name the publication that you must read and study to prepare for the ALAT certification exam. The Guide for the Care and Use of Laboratory Animals List the three levels of AALAS Technician Certification. Assistant Laboratory Animal Technician (ALAT), Laboratory Animal Technician (LAT), Laboratory Animal Technologist (LATg) What organization was formed in 1950 by Dr. Nathan Brewer and other veterinarians? Animal Care Panel (ACP) In 1967, the Animal Care Panel (ACP) changed it's name to what? American Association for Laboratory Animal Sciences (AALAS) What does the acronym RALAT mean? Registered Assistant Laboratory Animal Technician What does the acronym RLAT mean? Registered Laboratory Animal Technician What does the acronym RLATg mean? Registered Laboratory Animal Technologist What does the Principle Investigator do? Plans and coordinates all phases of the research work and prepares the protocol What is an Animal Use Protocol? A document written by the Principal Investigator and approved by the IACUC, that describes and justifies the use of animals for a project What does the acroynm IACUC mean?

International Animal Care and Use Committee What is the PHS Policy? Federal mandate that covers all live vertebrates used or intended for use in research, research training, experimentation, biological testing, or for related purposes. According to the Guide and PHS Policy, what document must institutions comply with when animals are euthanized? AVMA Guidelines for Euthanasia Using 100 mice in a research study instead of 150 could be an example of which of the 3 R's? Reduction What type of device identifies individuals using distinct characteristics such as fingerprints or eye structures? Biometric Scanner What document should be posted throughout the facility, and is consulted and used when there is an emergency? Contact list What federal agency enforces the rules and standards for workplace safety? Occupational safety and Health Administration (OSHA) Should a pregnant employee be assigned to work with cats? Why or why not? No. Cats can carry the microbes that cause toxoplasmosis, which can harm the fetus List three important factors for personal protection against radiation. Time; distance; shielding What is Toxoplasmosis gondii? A parasite that can be transmitted through cat feces. What is a decibel? A unit which measures loudness What are Material Safety Data Sheets? Provides information on toxicity and potential side effects, and describes recommended treatment for exposures.

What is zoonosis? Disease that can be transmitted from animals to humans What is a N95? A respirator mask that protects against airborne particles. Q-Fever is carried by what animal? Sheep (Ovis Aries) B virus is carried by what animal? Macaque Toxoplasmosis gondii is carried by what animal? Cat (Felis Cattus) Tuberculosis is carried by what animal? Nonhuman primate (NHP) Who submits the Animal Welfare Assurance of Compliance that the animal research program meets all relevant regulations and standards? Institutional Official What nonprofit organization accredits laboratory animal research programs? Association for the Assessment and Accreditation of Laboratory Animal Care International (AAALAC) What guidelines must an animal facility follow when research animals are euthanized at the end of a study? AVMA Guidelines on Euthanasia Beyond food, water, shelter, and healthcare requirements, to comply with the Animal Welfare Act, what must be provided to dogs in a laboratory animal research facility? Opportunities for exercise What does the acronym SOP stand for? Standard Operating Procedure What are the 3 Rs? Replacement, refinement, reduction How often must an IACUC perform inspections of the facility?

Every 6 months Who has the authority to sign the PHS Assurance? The Institutional Official Who reviews all animal use protocols at the institution before studies using animals can begin? International Animal Care and Use Committee (IACUC) What regulation applies to animal and non-animal research and safety studies funded or reviewed by the FDA and the EPA? Good Laboratory Practices (GLPs) Records of USDA inspections are available to the public under a provision of which federal law? Freedom of Information Act (FOIA) What document contains information regarding potential disasters, evacuation, and emergency contacts? Disaster plan What is the best way to measure the effectiveness of an institution-wide emergency system? Periodic disaster drills What involves the development of methods for uniquely recognizing humans based upon physical features? Biometrics If you are present in the facility when a major disaster or accident occurs, what should be your first priority? Protecting human life What does an eye wash station use to clean the eye? Clean water under low pressure What type of hazard causes musculoskeletal injuries Ergonomic What provides information on the toxicity of chemicals? Material Safety Data Sheets (MSDS) Whose standards specifically address the storage and handling of radioactive materials in the facility? Nuclear Regulatory Commission (NRC)

Adult sheep may consume up to how many gallon(s) of water a day? 1 gallon The average life span of a goat is how many years? 8 - 12 years, and sometimes as old as 15+ The color of rabbit urine is determined by what? The amount of minerals in the urine Every six months , a female cat will have several periods of estruses, which may last 3-6 days, reproductively she is said to be what?: Seasonally polyestrus A female cat is called what? Queen A male cat is called what? Tom A male goat is called what? Buck A female goat is called what? Doe A female sheep is called what? Ewe A male sheep is called what? Ram A female dog is called what? Bitch A male dog is called what? Dog What is the scientific name for a goat? Capra hircus What is the scientific name for sheep?

Ovis aries What is the scientific name for common house mouse? Mus musculus What is the scientific name of an African Clawed Frog? Xenopus laevis What is the scientific name for a cat? Felis cattus What is the scientific name for a dog? Canis familiaris What is the scientific name for cattle? Bos taurus What is the scientific name for a zebrafish? Danio rerio What is the scientific name for an axolotl? Ambystoma mexicanum What is the scientific name for a horse? Equus caballus What is the scientific name for a common rat? Rattus norvegicus What is the scientific name for a Syrian hamster? Mesocricetus auratus What is the scientific name for a rabbit? Oryctolagus cuniculus What is the scientific name for a guinea pig? Cavia porcellus What is the scientific name for a ferret? Mustela putorius furo

What regulation requires that dogs be given the opportunity for additional exercise outside of the normal cages or runs? Animal Welfare Act (AWA) Guinea Pigs are used in a number of infectious disease studies, but they are especially useful in the study of what disease? Tuberculosis Guinea pigs cannot synthesize what vitamin? Vitamin C Scurvy a disease caused by a deficiency of vitamin C. Why do gerbils have relatively little odor compared to other rodents? They produce very small amounts of urine Where is the ventral marking gland in Gerbils located? The abdomen Why are gerbils commonly used in genetic studies? Their chromosomes are easily observed. What do rabbits need while giving birth? A nest box What is the reddish material found in the tears and saliva of rats is called? Porphyrin The most unusual anatomic feature of the hamster, which makes it a popular model for research is called? Cheek pouch Euthanasia is listed in the AVMA Report as "Acceptable IV", this means what? It is the preferred method of administering the drug. An animal technician should assess the health of all animals under his/her care during what part of the day? Early in the morning

What does the term subcutaneous mean? Under the skin The Largest government agency fostering biomedical research with direct financial support is? The National Institutes of Health (NIH) What cellular structure contains the genetic material? The nucleus What is the recommended width for a hallway in animal facility? 6 - 8 feet Where should microisolator cages be changed? In a ventilated work bench The Panel of Euthanasia Report which lists methods and guidelines on animal euthanasia is published by who? American Veterinary Medical Association (AVMA) Brown fat A special fat that is found in newborns and some young adult animals that can be metabolized very rapidly to provide heat to increase or maintain body temperature. How often must nonhuman primates be observed during ground transportation? Once every 4 hours The "true stomach" in Ruminant is known as what? Abomasum What is the term for a specific causative agent of disease, such as a virus or bacterium? Pathogen Fomite A non-living material that can transmit a disease. Vector Any living thing that carries a disease When referring to disease , what does sub-clinical mean? Illness which does not make an animal sick

The USDA annual report must be submitted by what date each year? December 1st What federal agency regulates and publishes guidelines for the use of biohazards? Center for Disease Control and Prevention (CDC) What is not a physical method of euthanasia? Carbon dioxide A male ferret is called? A hob A female ferret is called? A Jill What is the term for eating feces? Coprophagy What are animals that are mostly active at night called? Nocturnal Porphyrin The reddish material found in the tears and saliva of rats. The hooded rat is also known as what? Long-Evans The Syrian hamster is also known as what? the golden hamster What is the normal color of hamster urine? Whitish What do you call an animal that is born blind and helpless? Altricial What is the most common out-bred guinea pig used in research? Dunkin-Hartley What is the normal gestation period for a cat? 2 months

A female guinea pig is called what? Sow A male guinea pig is called what? buck What is the unique reproductive trait among gerbils? They mate for life. What is parturition in rabbits called? kindling What is the normal gestation period for dogs? 2 months Why do rabbits ingest night feces? to recover B vitamins, proteins and water What is the scientific term used to describe blood in urine? hematuria How long is the gestation period for rabbits? 1 month What are animals who chew their cud are also know as? Ruminants What structure is used to determine the sex of bullfrogs? External Tympanic Membrane What anatomic feature is normally seen in New World monkeys? nostrils open to the front or sides What viral disease carried by macaques that can be fatal to humans? herpes B What is the most commonly used drug for nonhuman primate restraint? Ketamine What type of sling holds a pig in a fleece-lines hammock, suspended from the floor? Panepinto

What are young female pigs called? Glits What is term for parturition in swine is called? farrowing What is the scientific study of animal behavior is known as? Ethology How often should sheep hooves be trimmed? Every 3 months (quarterly) How many weeks are mice and rats gestation periods? 3 weeks (21 days) What are the non-functional pendants of skin sometimes hanging from either side of the goats neck? Wattles What is the space between the anus and genital papilla of rodents used for sexing? anogenital distance Amphibian cages should be routinely rinsed a few hours after what? feeding What is the best method to permanently identify a nonhuman primate? Chest tattoo What are the two main types of nonhuman primates? Anthropoids and prosimians What are Nonhuman primates who originate in South and Central America called? New World Monkeys A common condition in salamanders where they remain in their aquatic larval form with persistent gills throughout their entire life is called? Neoteny What is the common name for Xenopus laevis? African clawed frog What are the three parts of the avian reproductive system?

Cloaca, infundibulum, and magnum What is a special dietary component for birds that is composed of oyster shell and granite? Grit What is the clinical term for insufficient oxygen? Hypoxia What is the term used to describe the study of birth defects? Teratology Who prepares the "Guide for the care and Use of Laboratory Animals"? ILAR (Institute of Laboratory Animal Resources) What is it called if an animal lacks a means of generating heat internally? Ectotherm What do all organic compounds contain? Carbon What is the standard symbol for the element calcium? Ca What is the correct formula for the conversion of the Fahrenheit degrees to Celsius degrees? C=5/9 (F-32) The anatomic term ventral means towards the what? Abdomen The anatomic term medial means towards the what? middle of the body The kidneys are part of which body system? Urinary What is the function of the lungs? Deliver oxygen to the blood How many compartments are in a ruminant stomach? Four What is the largest vein in the body?

Vena cava In the gonads, sex cells divide and the daughter cells contain only half the number of chromosomes as the parent cells, this division is called? meiosis What type of caging material that has high impact strength, is transparent, and can withstand high temperatures? Polycarbonate What is another term for gnotobiotic animals? ... What is Alopecia defined as? Hairloss What type of drug relieves pain? Analgesic What government agency is in charge of preparing standards and enforcing the Animal Welfare Act? United States Department of Agriculture (USDA) In a rodent-breeding colony, which type of breeding scheme should be used to obtain minimal homozygosity in the shortest period of time? outbreeding Clean rodent barrier rooms should be ventilated with what type of pressure towards the hallway? Positive pressure The destruction of all organisms on an object is referred to as what? Sterilization An unusual behavior that may be noticed in approximately 20% of gerbils is what? Seizures What is the scientific name for swine? Sus Scrofa Domestica What is the scientific name for the Rhesus Macaque? Macaca Mulatta

What is the scientific name for baboons? Papio What is the scientific name for Orangutans? Pongo What is the scientific name for Squirrel Monkeys? Saimiri What is the scientific name for marmosets? Callithrix What is the scientific name for Tamarins? Sanguinus What is an engineering standard? A guideline that specifies in detail a method or technique for achieving a desired outcome. How does The Guide describe a Policy? A practical statement of collective wisdom. Animals on a fluid regulation protocol should have their body weights recorded at least how often? Once a week What is the recommended number of air changes in an hour in an animal room? 10 - 15 exchanges/hour What condition can occur in susceptible animals due to high room illumination levels? Phototoxic retinopathy What is the max sound level above which both auditory and non-auditory effects can result? 85 decibels What condition in mice and rats causes necrotic bands to appear on the tail as a result of vascular strictures caused by exposure to high temp and low relative humidity (less than 30%)? Ringtail What is environmental enrichment? A method of providing animals with the opportunity to behave as they do in the wild, playing, foraging, grooming, and interacting with one another.

Providing nude mice with cotton nesting material may lead to which condition? Conjunctivitis. What type of wood shavings are not recommended as bedding because it emits aromatic hydrocarbons? Cedar In the metric system, the prefix "centi" means? One hundredth What step is necessary when removing amphibians from shipping crates? Rinse thoroughly with Dechlorinated water to remove any Feces In general what is thought to be good environmental enrichment for amphibians? Hiding places What diseases in rats naturally develop, making them good animal models to study these diseases? Diabetes and hypertension Where are the testicles located in a male bird? Near the kidneys What causes rabbit urine to be red to milky yellow? High amounts of minerals in the urine A 4 kg cat weighs how many pounds? 8.8 pounds The act of shivering or sweating in order to maintain body temperature is an example of: Homeostasis A kilogram is approximately equal to how many pounds? 2.2 pounds What is the oldest inbred strain of mouse? DBA What metabolic disease is hereditary in hamsters? Diabetes mellitus What is another name for Feeding free choice? ad Libitum

What is a baby rabbit called? A kit What rat was the common laboratory rat developed from? The Brown Norway rat What is a knockout mouse? A mouse that results from the removal of one or more genes from the fertilized egg What is a method of correcting defective genes as a means of correcting a genetic disease? Gene therapy What is the shelf life for diets containing vitamin C? 90 days What is a transgenic animal? An animal that results from introduction of DNA from one animal into the egg of another animal. What is dystocia? Slow or difficult labor or birthing