Biochemistry: Organization of Cells and Reactions - Study Guide | CHEM 3550, Study notes of Biochemistry

Download Biochemistry: Organization of Cells and Reactions - Study Guide | CHEM 3550 and more Study notes Biochemistry in PDF only on Docsity! CHEMISTRY 3550 REVIEW Sheet & STUDY GUIDE-- EXAM #1 Chapters 1- 6; also 7.1, 7.3 - 7.5 & 7.7,7.8 SPRING 2011 Chapter 1: Biochemistry/Organization of Cells 1. Three primary areas of biochemistry 2. Know organic/biochemistry functional groups, Know the elements that predominate in biomolecules 3. Be familiar with general theory of the origins of biomolecules; Outcome of the Miller-Urey experiment 4. Have a general understanding of nature of biopolymers, their formation from monomeric building blocks (elements  supramolecular assemblies (organelles), etc). 5. Know basic differences between Prokaryotic vs Eukaryotic cells. Chapter 2: Water & Biochemical Reactions 1. Structure and properties of water (polarity); self-ionization reactions, biological roles of water (solvent properties); etc 2. Know/understand How biomolecules interact in/with water ( Hydrogen bonding, other noncovalent interactions) 3. Biochemical Acids/bases (biological); acid strengths; what are their properties? 4. Know/ understand the meaning: [H+] ion and pH; Ka and pKa 5. Know what are titration curves; how to interpret them; etc 6. Henderson-Hasselbalch equation, and how to manipulate it; plus basic calculations. 7. What are buffers; how do they work, how to prepare a buffer. 8. What buffer systems are present in living organisms (mammals). 9. Significance of intermolecular, noncovalent interactions in biochemistry. Chapter 3: Amino acids/Peptides 1. Basic Structure of amino acids, stereochemistry, 3-D structure, their names + 3-letter symbol. 2. Be familiar with amino acid R groups that comprise the main classification categories. 3. Understand the impact side chain classification may have on overall peptide/protein structure 4. Acid/base properties (titration) of Amino acids; charge dependence on pH; zwitterions, pI calculations? 5. Formation of and basic properties of the peptide bond; 3-D (planar structure) 6. Be aware of Small peptides of biological interest discussed in class. 7. Basic strategies for biochemical analysis of AA mixtures? Chapter 4: The 3-D structure of Proteins 1. The 4 levels of protein structure; forces holding them together; overall significance of the primary sequence/structure . 2. Types; properties and characteristics of main forms of Secondary structure; the bonding interactions involved; what is Supersecondary structure. 3. Characteristics of tertiary structure, protein folding schemes; roles of Chaperones, etc. 4. Features involved in protein quaternary structure; bonding types. 5. Two main types of proteins based on solubility & structure. 6. Basic comparison of structure and function differences/similarities between Myoglobin vs Hemoglobin. 1