Enzyme Activity and Cellular Processes in Biology, Exams of Nursing

Download Enzyme Activity and Cellular Processes in Biology and more Exams Nursing in PDF only on Docsity! BSC 1010 L EXAM 2 2024/2025  We worked with what enzyme? - Precise Answer ✔✔Salivary amylase What does Salivary amylase do ? - Precise Answer ✔✔An enzyme secreted by the salivary glands that begins the breakdown of complex sugars and starches. In the serial dilutions what was the substrate for these enzymes? - Precise Answer ✔✔6 What reagent was used to test enzymes? - Precise Answer ✔✔Benedicts reagent Lightly colored towards the center and green on the outside, there is no starch on the light parts of it. - Precise Answer ✔✔Coleus leaf How did temperature affect enzyme activity? - Precise Answer ✔✔if the temp is to high the enzyme will denature Hypertonic solution - Precise Answer ✔✔one with a higher concentration of solutes outside the cell than inside the cell. When a cell is immersed into this solution, the tendency is for water to flow out of the cell in order to balance the concentration of the solutes. hypotonic solution - Precise Answer ✔✔any solution that has a lower osmotic pressure than another solution. In the biological fields, this generally refers to a solution that has less solute and more water than another solution. isotonic solution - Precise Answer ✔✔refers to two solutions having the same osmotic pressure across a semipermeable membrane. This state allows for the free movement of water across the membrane without changing the concentration of solutes on either side. The leaf that was in the dark and did not react with the iodine because all of the iodine was used up the leaf that was exposed to the light turned dark brown almost black because it had not used all of its starch. - Precise Answer ✔✔Geranium Leaf Instrument used to detect wave length and absorbancy - Precise Answer ✔✔spectrophotometer How do you read a spectrophotometer? - Precise Answer ✔✔you put it in a sample holder called a cuvette and place it in the spectrophotometer. Light of a particular wavelength passes through the solution inside of the cuvette and the amount of light transmitted (passed through the solution) or absorbed by the solution is recorded. You are interested only in the absorbance of the compound you are studying. To determine how much light is absorbed by the solvent and other components in the solution, we compare the absorbance of our test solution to a reference blank.