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A comprehensive overview of the key concepts and principles in genetics and molecular biology. It covers topics such as the structure and function of dna, gene expression, genetic inheritance, and cell division processes like mitosis and meiosis. The document delves into the historical discoveries that laid the foundation for our understanding of heredity and the flow of genetic information, including the work of scientists like hershey, chase, watson, and crick. It also explores important genetic phenomena like mutations, gene flow, and mendelian inheritance patterns. The level of detail and breadth of topics covered make this document a valuable resource for students and researchers in the fields of biology, genetics, and molecular biology.
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selective breeding (artificial selection) - .......ANSWERS ๐ท๐โโthe process of developing organisms with specific characteristics as chosen by the breeders Gregor Mendel (1822-1884) - .......ANSWERS ๐ท๐โโAmateur botanist who published an explanation of hereditary transmission in plants in 1866. Known for his pea-plant experiments and commonly referred to as the "father of genetics" modern genetics - .......ANSWERS ๐ท๐โโthe study of heredity and the variation of inherited characteristics bacterial transforming principle - .......ANSWERS ๐ท๐โโan experiment proposed by Frederick Griffith in 1928 which suggested that a "transforming principle" from a heat- killed virulent Pneumococcus strain can transform a non-virulent strain into a pathogenic one. Avery, McCarty, MacLeod (1944) - .......ANSWERS ๐ท๐โโbiological researchers who identified DNA as the likely transforming principle in Griffith's experiment. Hershey and Chase (1952) - .......ANSWERS ๐ท๐โโconcluded that the genetic material of the bacteriophage was DNA, not protein. Edwin Chargaff - .......ANSWERS ๐ท๐โโAustrian biochemist who discovered that identical quantities of A and T, C and G were present in DNA (developed the complementary base-pairing rule for DNA). Frederick Griffith (1928) - .......ANSWERS ๐ท๐โโBritish bacteriologist; the first person to show that hereditary information could be transferred from one cell to another horizontally rather than vertically
genome - .......ANSWERS ๐ท๐โโthe complete set of genetic information carried by a species vertical transmission - .......ANSWERS ๐ท๐โโgene transmission between organisms without parental reproduction horizontal transmission - .......ANSWERS ๐ท๐โโgene transmission from parents to their offspring through reproduction (aka person-to-person) DNA replication - .......ANSWERS ๐ท๐โโthe process in which DNA makes a duplicate copy of itself. transcription - .......ANSWERS ๐ท๐โโsynthesis of an RNA molecule from a DNA template mRNA (messenger RNA) - .......ANSWERS ๐ท๐โโa single-stranded RNA molecule that encodes the information to make a protein hereditary material - .......ANSWERS ๐ท๐โโthe information which is passed from one cellular generation to the next (encoded in DNA in humans). Pauling and Corey (1951) - .......ANSWERS ๐ท๐โโbiological researchers who provided the basis for research on DNA structure (proposed an alpha helix model as a structure for nucleic acid) Watson and Crick (1953) - .......ANSWERS ๐ท๐โโthe two scientists who discovered the structure and shape of DNA nucleotide - .......ANSWERS ๐ท๐โโa building block of DNA, consisting of a five-carbon sugar covalently bonded to a nitrogenous base and a phosphate group. genes - .......ANSWERS ๐ท๐โโDNA segments that serve as the key functional units in hereditary transmission.
chromosomes - .......ANSWERS ๐ท๐โโa threadlike structure of nucleic acids and protein found in the nucleus of most living cells, carrying genetic information in the form of genes. nucleoid - .......ANSWERS ๐ท๐โโa dense region of DNA in a prokaryotic cell. diploid - .......ANSWERS ๐ท๐โโcontaining two complete sets of chromosomes, one from each parent (2n) haploid - .......ANSWERS ๐ท๐โโan organism or cell having only one complete set of chromosomes (n) gametes - .......ANSWERS ๐ท๐โโhuman sex cells containing one set of 23 chromosomes homologous chromosomes - .......ANSWERS ๐ท๐โโmatched pairs of chromosomes in a diploid organism Chargaff's Rule - .......ANSWERS ๐ท๐โโA=T and C=G locus - .......ANSWERS ๐ท๐โโposition of a gene on a chromosome Meselson and Stahl (1958) - .......ANSWERS ๐ท๐โโBacterial cells were grown in a heavy isotope of nitrogen, 15N All the DNA incorporated 15N Cells were switched to media containing lighter 14N DNA was extracted from the cells at various time intervals central dogma - .......ANSWERS ๐ท๐โโtheory that states that, in cells, information only flows from DNA to RNA to proteins
translation - .......ANSWERS ๐ท๐โโdecoding of a mRNA message into a polypeptide chain initiation of translation - .......ANSWERS ๐ท๐โโmRNA is attached to a subunit of the ribosome, the first codon is always AUG template strand - .......ANSWERS ๐ท๐โโthe strand of DNA that specifies the complementary mRNA molecule nontemplate strand - .......ANSWERS ๐ท๐โโThe un-transcribed partner of the template strand of DNA used in transcription. RNA polymerase - .......ANSWERS ๐ท๐โโenzyme that links together the growing chain of RNA nucleotides during transcription using a DNA strand as a template. polypeptide - .......ANSWERS ๐ท๐โโlong chain of amino acids that makes proteins amino acids - .......ANSWERS ๐ท๐โโbuilding blocks of proteins triplet codon - .......ANSWERS ๐ท๐โโthree nucleotide sequence on mRNA that codes for specific amino acid start codon - .......ANSWERS ๐ท๐โโcodon that signals to ribosomes to begin translation; codes for the first amino acid in a protein (AUG) stop codon - .......ANSWERS ๐ท๐โโUAG, UAA, or UGA; the codon that ends all RNA sequences. Charles Darwin (1809-1882) - .......ANSWERS ๐ท๐โโEnglish naturalist who postulated a theory of evolution by natural selection. Alfred Wallace (1823-1913) - .......ANSWERS ๐ท๐โโ"Father of Biogeography," was the first to propose that animal species have "geographies"
natural selection - .......ANSWERS ๐ท๐โโa process in which individuals that have certain inherited traits tend to survive and reproduce at higher rates than other individuals because of those traits. descent with modification - .......ANSWERS ๐ท๐โโprinciple that each living species has descended, with changes, from other species over time mutation - .......ANSWERS ๐ท๐โโa random error in gene replication that leads to a change silent mutation - .......ANSWERS ๐ท๐โโa mutation that changes a single nucleotide, but does not change the amino acid created. missense mutation - .......ANSWERS ๐ท๐โโa point mutation in which a codon that specifies an amino acid is mutated into a codon that specifies a different amino acid. nonsense mutation - .......ANSWERS ๐ท๐โโa mutation that changes an amino acid codon to one of the three stop codons, resulting in a shorter and usually nonfunctional protein. frameshift mutation - .......ANSWERS ๐ท๐โโa mutation that shifts the "reading" frame of the genetic message by inserting or deleting a nucleotide insertion - .......ANSWERS ๐ท๐โโa mutation involving the addition of one or more nucleotide pairs to a gene. deletion - .......ANSWERS ๐ท๐โโa change to a chromosome in which a fragment of the chromosome is removed. genetic drift - .......ANSWERS ๐ท๐โโa change in the allele frequency of a population as a result of chance events rather than natural selection.
gene flow (migration) - .......ANSWERS ๐ท๐โโmovement of alleles from one population to another nonrandom mating - .......ANSWERS ๐ท๐โโmating among individuals on the basis of their phenotypic similarities or differences, rather than mating on a random basis point mutation - .......ANSWERS ๐ท๐โโgene mutation involving changes in one or a few nucleotides. degeneracy - .......ANSWERS ๐ท๐โโredundancy of the genetic code; that is, most amino acids are encoded by several codons conditional mutation - .......ANSWERS ๐ท๐โโa mutation that results in a characteristic phenotype only under certain environmental conditions. assortative mating - .......ANSWERS ๐ท๐โโthe type of mating that occurs when an organism selects a mating partner that resembles itself dissassortative mating - .......ANSWERS ๐ท๐โโphenotypically different individuals mate, producing an excess of heterozygotes genetic variance - .......ANSWERS ๐ท๐โโdiversity of alleles and genotypes in a population inbreeding - .......ANSWERS ๐ท๐โโbreeding from closely related people or animals, especially over many generations. analogy - .......ANSWERS ๐ท๐โโsimilarity of function and superficial resemblance of structures that have different origins (e.g. wings of a fly, moth and bird) homoplasy - .......ANSWERS ๐ท๐โโwhen a trait has been gained or lost independently in separate lineages over the course of evolution
phylogenetic tree - .......ANSWERS ๐ท๐โโa family tree that shows the evolutionary relationships thought to exist among groups of organisms cladistic approach - .......ANSWERS ๐ท๐โโa method for constructing a phylogenetic tree that is based on identifying the unique traits of each monophyletic group. clades - .......ANSWERS ๐ท๐โโa group of organisms believed to have evolved from a common ancestor, according to the principles of cladistics. monophyletic group - .......ANSWERS ๐ท๐โโa taxonomic grouping that includes an ancestral species and all of its descendants. ancestral characteristics - .......ANSWERS ๐ท๐โโcharacteristics that have arisen in organisms as a result of common evolutionary descent derived characteristics - .......ANSWERS ๐ท๐โโcharacteristics that appear in recent parts of a lineage but not in its older members maximum parsimony - .......ANSWERS ๐ท๐โโ"Occam's Razor" a principle that states that when considering multiple explanations for an observation, one should first investigate the simplest explanation that is consistent with the facts. In Griffith's experiments, what made the harmless R cells grow capsules when exposed to heat-killed S cells? - .......ANSWERS ๐ท๐โโDNA In the Hershey-Chase experiment radioactive Sulfur was used to: - .......ANSWERS ๐ท๐ โโBe incorporated into the phage protein coat because some amino acids contain sulfur The experiments by Hershey and Chase helped confirm that DNA was the hereditary material on the basis of the finding that: - .......ANSWERS ๐ท๐โโRadioactive phosphorus was found inside the cell
If DNA of a particular species was analyzed and it was found that it contains 27 percent of adenine (A), what would be the percentage of cytosine (C)? - .......ANSWERS ๐ท๐โโ23 percent Four nucleic acid samples are analyzed to determine the percentages of the nucleotides they contain. Which of the following DNA sample(s) could be double- stranded DNA? Sample 1: A(22%), G(28%), T(22%), U(0%), C(28%); Sample 2: A(30%), G(30%), T(0%), U(20%), C(20%); Sample 3: A(18%), G(32%), T(0%), U(18%), C(32%); Sample 4: A(29%), G(29%), T(21%), U(0%), C(21%) - .......ANSWERS ๐ท๐โโSample 1 Your chromosomes are located _______ and they are made of ___________.
Where does translation take place in eukaryotic cells? - .......ANSWERS ๐ท๐ โโCytoplasm Transcribe the following DNA sequence (non template strand): 5'- ATGGCCGGTTATTAAGCA-3' - .......ANSWERS ๐ท๐โโ5'-AUGGCCGGUUAUUAAGCA-3' In which of the following pairs do both evolutionary processes introduce new genetic variation into a population? - .......ANSWERS ๐ท๐โโMutation and gene flow Mutation can be defined as the addition of genetic variation that increases the hereditary diversity of a population. - .......ANSWERS ๐ท๐โโTrue If an allele has a frequency of 1: - .......ANSWERS ๐ท๐โโIt is found in 100% of the population When male lions reach sexual maturity, they leave their group in search of a new pride. This can alter the allele frequencies of the population through which of the following mechanisms? - .......ANSWERS ๐ท๐โโGene flow When closely related individuals mate with each other, or inbreed, the offspring are often not as fit as the offspring of two unrelated individuals. Why? - .......ANSWERS ๐ท๐โโInbreeding can bring together rare, deleterious mutations that lead to harmful phenotypes. Which statement about analogies is correct? a. They occur only as errors. b. They are synonymous with homologous traits. c. They are derived by similar environmental constraints. d. They are a form of mutation. - .......ANSWERS ๐ท๐โโC What do scientists use to apply cladistics? - .......ANSWERS ๐ท๐โโhomologous traits What is true about organisms that are a part of the same clade?
a. They all share the same basic characteristics. b. They evolved from a shared ancestor. c. They usually fall into the same classification taxa. d. They have identical phylogenies. - .......ANSWERS ๐ท๐โโB Why do scientists apply the concept of maximum parsimony? - .......ANSWERS ๐ท๐ โโto decipher accurate phylogenies Alternate forms of a gene are called _______. - .......ANSWERS ๐ท๐โโalleles Describing a person as having hemophilia is to indicate his or her ______.
The presence of short hairs on leaves is controlled by dominant allele H. Plants with genotype hh have smooth leaves. Two parental plants with unknown genotype were crossed. What are the parental genotypes if F1= 32 Hairy and 11 smooth?
information, John's parents are: - .......ANSWERS ๐ท๐โโBoth heterozygous for the alkaptonuria gene. If John marries someone who is heterozygous for alkaptonuria, then which of the following statements is true? Remember alkaptonuria is a recessive disorder.
experiment - .......ANSWERS ๐ท๐โโa planned operation carried out under controlled conditions event - .......ANSWERS ๐ท๐โโany combination of outcomes Addition Rule of Probability - .......ANSWERS ๐ท๐โโUsed to determine the probability that at least one of two mutually exclusive events will occur. P(A or B) = P(A) + P(B) - P(AB) multiplication rule - .......ANSWERS ๐ท๐โโA rule of probability stating that the probability of two or more independent events occurring together can be determined by multiplying their individual probabilities. P(A,B)=P(A)P(B) Conditional Probability - .......ANSWERS ๐ท๐โโthe probability of an event ( A ), given that another ( B ) has already occurred. Binomial Distribution - .......ANSWERS ๐ท๐โโThe probability distribution of X with parameters n and p true-breeding - .......ANSWERS ๐ท๐โโterm used to describe organisms that produce offspring identical to themselves if allowed to self-pollinate hybridization - .......ANSWERS ๐ท๐โโCrossing dissimilar individuals to bring together the best of both organisms parental generation - .......ANSWERS ๐ท๐โโthe adults used in the first experimental cross of a breeding experiment Mendel's Postulates - .......ANSWERS ๐ท๐โโ1) Genetic characters are controlled by unit factors that exist in pairs in individual organisms.
law of independent assortment - .......ANSWERS ๐ท๐โโgenes do not influence each other with regard to the sorting of alleles into gametes, and every possible combination of alleles for every gene is equally likely to occur. When fertilization occurs between two true-breeding parents that differ in only one characteristic, the process is called a ________. - .......ANSWERS ๐ท๐โโmonohybrid cross Punnett Square - .......ANSWERS ๐ท๐โโA chart that shows all the possible combinations of alleles that can result from a genetic cross test cross - .......ANSWERS ๐ท๐โโa way to determine whether an organism that expressed a dominant trait was a heterozygote or a homozygote autosomal inheritance - .......ANSWERS ๐ท๐โโThe inheritance patterns that occur when genes are located on autosomes rather than on sex chromosomes. autosomal dominant inheritance - .......ANSWERS ๐ท๐โโinheritance of a dominant gene mutation on an autosome Autosomal Recessive Inheritance - .......ANSWERS ๐ท๐โโtwo recessive alleles must be present for trait to be displayed cell cycle - .......ANSWERS ๐ท๐โโThe regular sequence of growth and division that cells undergo to produce two new daughter cells G1 phase - .......ANSWERS ๐ท๐โโstage of interphase in which cell grows and performs its normal functions (little change is visible). S phase - .......ANSWERS ๐ท๐โโThe synthesis phase of the cell cycle; the portion of interphase during which DNA is replicated. sister chromatids - .......ANSWERS ๐ท๐โโIdentical copies of a chromosome; full sets of these are created during the S phase
mitotic spindle - .......ANSWERS ๐ท๐โโthe apparatus that orchestrates the movement of chromosomes during mitosis G2 phase - .......ANSWERS ๐ท๐โโstage of interphase in which the cell replenishes its energy stores and synthesizes proteins necessary for chromosome manipulation To make two daughter cells, the contents of the ______ and the ______ must be divided
Cytokinesis - .......ANSWERS ๐ท๐โโDivision of the cytoplasm during cell division to form two separate daughter cells cleavage furrow - .......ANSWERS ๐ท๐โโthe first sign of cytokinesis during cell division in an animal cell; a shallow groove in the cell surface near the old metaphase plate Daughter cells produced by mitosis _______. a. are genetically identical to the parent cell b. are only created in females c. divide the cytoplasm into four parts d. cause cancer - .......ANSWERS ๐ท๐โโA Besides growth, what must happen before a cell divides? - .......ANSWERS ๐ท๐โโDNA replication The division of the cytoplasm following mitosis to form two new cells is called?
Meiosis - .......ANSWERS ๐ท๐โโCell division that produces reproductive cells in sexually reproducing organisms Recombination (crossing over) - .......ANSWERS ๐ท๐โโthe exchange of genetic material between homologous chromosomes during meiosis Meiosis 1: Prophase 1 - .......ANSWERS ๐ท๐โโHomologous chromosomes condense, pair up, and swap segments Spindle microtubules attach to chromosomes as the nuclear envelope breaks up chiasmata - .......ANSWERS ๐ท๐โโX-shaped regions where crossing over occurred. crossing over - .......ANSWERS ๐ท๐โโthe exchange of genes between homologous chromosomes, resulting in a mixture of parental characteristics in offspring. Meiosis 1: Prometaphase 1 - .......ANSWERS ๐ท๐โโthe attachment of the spindle fiber microtubules to the kinetochore proteins at the centromeres. Meiosis 1: Metaphase 1 - .......ANSWERS ๐ท๐โโhomologous chromosomes are arranged in the center of the cell with the kinetochores facing opposite poles. The homologous pairs orient themselves randomly at the equator. Meiosis 1: Anaphase 1 - .......ANSWERS ๐ท๐โโthe microtubules pull the linked chromosomes apart. The sister chromatids remain tightly bound together at the centromere. The chiasmata are broken in anaphase I as the microtubules attached to the fused kinetochores pull the homologous chromosomes apart. Meiosis 1: Telophase 1 and Cytokinesis - .......ANSWERS ๐ท๐โโnuclear membranes form, the cell separates into 2 cells Meiosis II - .......ANSWERS ๐ท๐โโthe second phase of meiosis consisting of chromatids separating, along with the two diploid cells splitting in two
Meiosis produces ________ daughter cells. - .......ANSWERS ๐ท๐โโfour haploid If a muscle cell of a typical organism has 32 chromosomes, how many chromosomes will be in a gamete of that same organism? - .......ANSWERS ๐ท๐โโ 16 Which of the following events is unique to prophase I of meiosis? - .......ANSWERS ๐ท๐ โโPairing of homologous chromosomes. At metaphase I, homologous chromosomes are connected only at what structures?
In a cross between a homozygous red-eyed female fruit fly and a white-eyed male fruit fly, what is the expected outcome? - .......ANSWERS ๐ท๐โโAll offspring with red eyes. In a cross between a white-eyed female fruit fly and a red-eyed male fruit fly, what is the expected outcome? - .......ANSWERS ๐ท๐โโAll male offspring with white eyes. What would a female genotype have to be for her to have the genetic disease hemophilia? - .......ANSWERS ๐ท๐โโBoth her X chromosomes would have to have the recessive hemophilia gene. Congenital generalized hypertrichosis is an X-linked dominant disease. If an affected male marries an unaffected female, what is the probability of passing the hypertrichosis gene to his offspring? - .......ANSWERS ๐ท๐โโp=0.5, all daughters will be affected. Hemophilia has an X-linked recessive pattern. It is carried on the "X" chromosome. It is only expressed phenotypically as homozygous recessive in women but men that inherit the affected chromosome will suffer from the disorder. A carrier woman (one affected X chromosome) married a man with hemophilia, and they have children. What percent of their boy children will be affected with hemophilia? - .......ANSWERS ๐ท๐ โโ50% of the boy children.