Hematology laboratory testing- Automated/Manual Questions And Answers, Exams of Hematology

Download Hematology laboratory testing- Automated/Manual Questions And Answers and more Exams Hematology in PDF only on Docsity! Hematology laboratory testing- Automated/Manual Questions And Answers A dilution commonly used for a routine sperm count is: - correct answer 1:20 A manual white blood cell count was performed by the hematology technologist. The cell counts for both sides were 99 and 164 respectively. All nine large squares were counted on each side. The dilution for this kit was pre-measured at 1:100. What should the technologist report as the white cell count? - correct answer Cannot report How many 1 mm x 1 mm squares is the Neubauer hemacytometer counting grid composed of? - correct answer Nine per side Twenty microliters (20 µL) of blood are diluted with 1.98 mL of diluent. Both sides of the hemocytometer are charged with the diluted sample and the entire ruled area of the center square is counted on both sides. A total of 356 platelets are counted (combined total of the two large squares). What is the platelet count per liter? - correct answer 178 x 109/L What would be the logical first step to obtain correct values when the following results were obtained on an electronic particle counter in hematology for an alcoholic patient? Hematology laboratory testing- Automated/Manual Questions And Answers WBC 6,500/cu. mmMCV 90.1 fLRBC 4,550,000/cu. mmMCH 39.6 pgHGB 18.0 g/dLMCHC 43.4%HCT 41.5% - correct answer Check and correct for lipemia If the spinal fluid is slightly hazy, what is the best dilution? - correct answer 1:10 A 1:10 dilution is made on a CSF sample. Five squares on each side of the hemacytometer are counted for a total of 10 squares and a total of 150 cells are recorded. What is the count per microliter? - correct answer 1500 Which of the following tests would be most affected by microclots in an EDTA tube drawn for a CBC? - correct answer Platelet count What is the BEST type of microscopy for performing manual platelet counts? - correct answer Phase contrast What is the optimal time to begin initial microscopic examination of a semen specimen, assuming liquefaction is complete? - correct answer 30 minutes Hematology laboratory testing- Automated/Manual Questions And Answers Charcot-Leyden crystals in stool may be associated with an immune response and are thought to be the breakdown products of: - correct answer Eosinophils A hemocytometer is generally used for all of the following, EXCEPT? - correct answer Synovial fluid crystal counts A manual platelet count was performed using a Neubauer hemacytometer. The total platelet count was 356 x 103/µL. A platelet estimate was then performed on the peripheral blood smear. Ten fields were counted and yielded the following results: 17, 16, 15, 20, 19, 17, 19, 16, 20, 18. The platelet estimation factor for the microscope used was 20,000. What is the next logical step? - correct answer Report the manual platelet count since it correlates well with the platelet estimate Which of the following formulas would you use to calculate absolute cell counts? - correct answer Total count X % of cells counted A complete blood count is performed. The white blood cell count is elevated and a manual differential is required. The technologist evaluates the red blood cell morphology and finds it "Normal." Which of the following histograms would represent a normal red blood cell morphology? - correct answer B Hematology laboratory testing- Automated/Manual Questions And Answers The site most frequently used to collect bone marrow samples from adults is: - correct answer Posterior iliac crest All of the following can cause erroneous automated platelet counts EXCEPT? - correct answer WBC fragmentation A sample of cerebrospinal fluid is diluted 1:100; the standard 9 squares of a hemocytometer were counted on each side for a total of 18 large squares. Side 1- 186 cells counted Side 2- 184 cells counted total cells = 370 What is the cell count per microliter? - correct answer 2.06 x 104 The image is a Wright-Giemsa stained smear (1000x) of cerebrospinal fluid (CSF). The arrows point to cells that may indicate which of the following conditions? - correct answer Leukemia with CNS involvement Which of the following represents the White Blood Cell (WBC) count reference range for adults? - correct answer 4.4 - 11.0 x 109/L Hematology laboratory testing- Automated/Manual Questions And Answers In a moderately bloody specimen, 250 cells are counted in 1 primary square. The dilution is 1:100. What is the count/µL? - correct answer 2.5 x 105 Two CSF specimens were sent to the Lab with the following results:Tube #1 = 11,200 rbc/µLTube #2 = 300 rbc/µLThe results on these CSF specimens are indicative of: - correct answer A traumatic tap Upon centrifugation, a cerebrospinal fluid (CSF) sample supernatant exhibited xanthochromia. The image is a Wright-Giemsa stained smear that was made from that CSF sample. What condition is probably related to these macroscopic and microscopic findings? - correct answer Previous subarachnoid hemorrhage You are working in a hematology laboratory and the machine flags an outlier result for a 51-year-old male. The blood was collected in an EDTA tube for a CBC. The hematocrit of the specimen is high at 60%. Which of the following would you do? - correct answer Report these results. Which of these descriptions correlates best with the red cell distribution curve shown? - correct answer Normal red cell distribution Hematology laboratory testing- Automated/Manual Questions And Answers Side 1-- 186 nucleated cells counted Side 2-- 184 nucleated cells counted Total nucleated cells = 370 Using the standard hemocytometer formula shown on the right, what is the nucleated cell count per microliter (µL)? - correct answer 2.06 x 104 A technologist performed a STAT spinal fluid count by pipetting undiluted spinal fluid in a Neubauer Counting Chamber and counting both sides of the chamber (4 large WBC squares on each side). A total of 54 White Cells were counted. How many White Cells would there be per cubic millimeter? - correct answer 67.5 Which of the following statements is FALSE? - correct answer Nucleated red blood cells can cause a falsely decreased white blood count. A manual white blood cell count was performed by the hematology technologist. The cell counts for both sides were 152 and 164 respectively. All nine large squares were counted on each side. The dilution for this kit was pre-measured at 1:100. What should the technologist report as the white cell count? - correct answer 17.5 x 109/L Hematology laboratory testing- Automated/Manual Questions And Answers What is a corrective action that can be used to obtain a valid platelet count from a patient specimen that exhibits platelet satellitism on the manual differential? - correct answer Recollect the blood specimen in a blue top tube containing sodium citrate Cerebrospinal fluid (CSF) specimens are usually collected by lumbar puncture and placed in numbered tubes starting with the first tube collected. If four tubes are collected, which tube would be used for a cell count and differential? - correct answer Tube #3 The image on the right is a representative field from a peripheral blood smear. What would you expect to see on the automated blood count? - correct answer MCV increased, RDW increased What does the M:E ratio in a bone marrow evaluation represent? - correct answer Myeloid cells to erythroid cells A white blood cell (WBC) count is performed on a blood specimen from a newborn using a hematology analyzer that does not have the capability to detect and quantify nucleated red blood cells (NRBCs). The WBC count that is reported by the analyzer is 28.2 x 109/L and it is flagged for a manual differential. Upon review of the blood film, more than 6 nucleated cells that resemble the cells shown in the image were identified in the 100 WBC differential. What action should be taken? - Hematology laboratory testing- Automated/Manual Questions And Answers correct answer Count the number of cells that resemble the cells in this image (per 100 WBCs) and compute for the corrected WBC count using the formula, Total WBCs x100 / 100+ number of NRBCs What is the corrected white blood cell count if the WBC is 14,460, and there were 47 nucleated red blood cells per 100 white blood cells noted on the differential count? - correct answer 9,837 What should be added to the cytocentrifuge chamber when preparing slides on serous fluids to aid in adhesion of cells to the slide and preserve cellular morphology? - correct answer 22% Albumin A WBC differential count on CSF should be performed using the following technique: - correct answer Stained smear of a cytocentrifuged specimen When performing a manual white blood cell count, 236 cells were counted on side number one of the hemacytometer, 224 cells on side number two. A 1/20 dilution of 1% HC1 was used and the area counted on each side was 4 sq. mm. Twenty-five nucleated red blood cells in the differential were observed per 100 white blood cells. What is the CORRECT WBC/µL? - correct answer 9,200