Human Enzyme - Advanced Technologies in Biochemistry and Cell Biology - Exam, Exams of Biochemistry

Download Human Enzyme - Advanced Technologies in Biochemistry and Cell Biology - Exam and more Exams Biochemistry in PDF only on Docsity! Ollscoil na hÉireann, Gaillimh GX_____ National University of Ireland, Galway Semester II Examinations, 2009/2010 Exam Code(s) 4BS2, 4BO2 Exam(s) BI401 Module Code(s) BI441 Module(s) Advanced technologies in biochemistry and cell biology Paper No. Repeat Paper Special Paper External Examiner(s) Prof. M. J. R. Stark Internal Examiner(s) Dr. S. Gundy, Dr. T. Higgins, Dr. A. Flaus, Dr. P. Creighton, Prof. K. Sullivan, Dr. G. Bedoux. Instructions: Write on three of the following topics, choosing at least one from each section. Please start each answer with a new answer book and clearly indicate the number on the front. Duration 3 hrs No. of Answer books 3 Requirements: Handout MCQ Statistical Tables Graph Paper Log Graph Paper Other Material No. of Pages 3 Department(s) Biochemistry Page 1 of 3 NATIONAL UNIVERSITY OF IRELAND, GALWAY Ollscoil na hÉireann, Gaillimh 2009-10 B.Sc. Degree Honours Examination BIOCHEMISTRY BI441 Advanced Technologies in biochemistry and cell biology External Examiner: Prof. M. J. R. Stark Time allowed: Three Hours Write on three of the following topics, choosing at least one from each section. Please start each answer with a new answer book and clearly indicate the number on the front. Section A 1. You have a protein that you want to purify and analyze. Describe one specific chromatographic mode of separation that you could use to purify the protein, and describe one electrophoretic separation technique that you could use to analyze the properties of the protein. 2. Describe how the x-ray crystallographer ensures quality at all stages in the process of determining the three-dimensional structure of proteins. 3. You have read a scientific report describing the enzymatic properties of a mouse protein and you wish to study the human homologue. Describe how you plan to identify, clone, express and purify the human enzyme. Page 2 of 3