Exam Instructions for Advanced Biochemistry & Cell Biology - Semester 2, 2011/2012, Exams of Biochemistry

Download Exam Instructions for Advanced Biochemistry & Cell Biology - Semester 2, 2011/2012 and more Exams Biochemistry in PDF only on Docsity! Page 1 of 3 Semester 2 Examinations 2011/2012 Exam Code(s) 4BS2, 4BO2 Exam(s) BI401 Biochemistry; Biomedical Science Module Code(s) BI441 Module(s) Advanced technologies in biochemistry and cell biology Paper No. I External Examiner(s) Prof. M.J.R. Stark Internal Examiner(s) Dr. A. Flaus Dr. P. Creighton Dr. A. McCarthy Dr P. Crowley Prof. K. Sullivan Dr. M. Tuohy Instructions: Answer three questions, choosing at least one from each section. Please start each answer with a new answer book and clearly indicate the number on the front. All questions carry equal marks. Duration 3 hrs No. of Pages 3 Discipline(s) Biochemistry Course Co-ordinator Dr. L. Byrnes Requirements None Release to Library Yes Page 2 of 3 BI441 Advanced technologies in biochemistry and cell biology Time allowed: Three Hours Write on three of the following topics, choosing at least one from each section. Please start each answer with a new answer book and clearly indicate the number on the front. Section A 1. Your supervisor has provided you with the sequence of a human gene. Explain how you would obtain a suitable template for PCR, and describe in detail the steps you would take to clone this PCR product into an E. coli recombinant protein expression plasmid. Explain how you would validate that the expected clone is correct. Illustrate your answer with diagrams wherever possible. 2. Answer either (a) Describe the typical strategy of determining a three dimensional structure of a macromolecule using X-ray crystallography. Or (b) Describe the application of NMR spectroscopy to study the structure and affinity of a protein-protein complex. Use the following headings as a guide: • Isotope enrichment • HSQC spectrum • Chemical shift perturbation mapping • Binding affinity determination 3. Explain how new technologies such as RNAi, bioinformatics and systems biology enable genome-wide investigations of molecular mechanisms in the cell. Illustrate your answer with diagrams wherever possible. 4. Explain what is meant by the term ‘next-generation sequencing’. Illustrate your answer by describing two next-generation sequencing technologies in detail.