Microbiology Lab Practical Exam 1 - Study Guide, Exams of Microbiology

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Wet mount steps: - Correct answer 1.water

2. Specimen
3. Cover slip over specimen Hanging drop steps: - Correct answer 1. Vaseline around cover slip
4. Microorganism in center of cover slip
5. Place concave slide on cover slip Gram stain steps: - Correct answer 1. Crystal Violet
   2. Gram's Iodine
6. Ethyl Alcohol
7. Sarasin Red Negative stain steps: - Correct answer 1. Ingrain
8. Heat loop
9. Bacteria
10. Slide to spread mixture
11. Air dry Acid-fast stain steps: - Correct answer 1. Carbon fussion
12. Acid alcohol
13. Methylene blue
14. Wash & dry Simple stain steps - Correct answer 1. Fixed smear slide
15. Dyes
16. Rinse & dry Nutrient broth - Correct answer -liquid media used to grow bacteria Agar - Correct answer -used to culture microorganisms

Candle jar - Correct answer -used for anaerobic organisms Petri dish - Correct answer -Used to culture or observe bacteria growth Slant - Correct answer -a medium for which bacteria can grow on. Escolar Gram stain - Correct answer red colored rods S. epidermis’s Gram stain - Correct answer purple colored grapes B. subtitles Gram stain - Correct answer purple colored rods List information gained from a Particular slide based on staining techniques (cell morphology, arrangement, cell wall structure, Gram stain reaction or other staining reaction). - Correct answer... Show motility on a hanging drop - Correct answer movement because of flagella Show motility on a Brownian movement. - Correct answer bacteria moves randomly, due to the energy of a system (vibration) Why does motility occur in bacteria? - Correct answer they have a flagellum that propels them around. Why does motility occur in protozoans? - Correct answer 1. Flagella 2. Cilia 3. Amoeba Aseptic technique steps - Correct answer 1. Flame loop

2. Flame tube
3. Inoculate bacteria
4. Flame loop
5. Flame tube

Solid culture set up - Correct answer 1- water 2-heat loop 3-bacteria 4-air dry 5-apply heat Broth culture set up - Correct answer 1. Heat loop

2. Bacteria
3. Air dry
4. Heat fix
5. Methanol
6. Air dry
7. Heat fix How do you properly dry and fix a slide? - Correct answer -heat loop

• add bacteria -air dry -heat FIX What can happen to a Gram stain if a smear is too heavy? - Correct answer can't see cells What happens to a Gram stain if a smear is too light? - Correct answer cells are scattered away or not enough Flatworm - Correct answer -has gastro vascular cavity, functions as both mouth and anus
• Locomotion involves gliding Roundworms - Correct answer -has a complete digestive tract, separate openings for the mouth and anus How is biofilm formed - Correct answer - attaching and growing on surface when exposed to water

Fermentation tubes - Correct answer -detects acid and gases from carbohydrates -phenol red indicates alkaline -yellow indicates acid production -clear Durham tubes indicate gases Methyl red test (M.R test) - Correct answer organisms make acid from glucose

• Red/orange is positive -yellow is negative Voges-Proskauer (VP test) - Correct answer -organisms utilizing butylene glycol to make action -red color on top is a positive Citrate test - Correct answer - organism use it for carbon & energy. -Negative- green -Positive-blue Gel hydrolysis - Correct answer - organisms use the protein gelatin for carbon and energy growth. -Positive: liquid -Negative: solid Insole production - Correct answer -organisms convert tryptophan (amino acid) to insole
• Positive result is a red color on the surface Urease test - Correct answer - organisms hydrolyze urea to make ammonia and CO
• Pink is positive -yellow is negative Phenylalanine deamination test - Correct answer -organisms producing the enzyme deaminize

• Dark green (positive result.) Hydrogen sulfide production - Correct answer organisms that reduce sulfur- containing compounds to sulfides. Black precipitate is the result MIO test - Correct answer -determines motility of microorganisms -ornithine decarboxylase activity and insole production. -motility is indicated by cloudiness
• Red is positive for insole. -purple is positive for ornithine Oxidase test - Correct answer organism that make cytochrome c oxidase Positive reactions is blue/purple Catalase test - Correct answer enzyme that breaks down h2O2 to O2 and H2O -positive = bubbling. -negative = no bubbles Know how to lower the oxygen content so that anaerobes can grow. - Correct answer -use a candle jar -use an anaerobic jar Know where bacteria will grow in a tube of solid medium based on oxygen requirements. - Correct answer -obligate aerobes- grow on top -Obligate anaerobes-grows on bottom -Microaerophiles- grows between bottom and top What’s the purpose of using dilution techniques to isolate bacteria? - Correct answer isolates bacteria Streak plate - Correct answer -heat loop

-scoop broth once and streak quadrant 1 -heat loop -streak quadrant 2 -heat loop -quadrant 3 -heat loop -quadrant 4 Streak plate vs. pour plate dilution. - Correct answer -produces individual colonies on an agar plate -determines the number of microbes in a specimen Pellicle nutrient broth tube - Correct answer Flocculent nutrient broth tube - Correct answer Sediment nutrient broth tube - Correct answer Turbidity nutrient broth tube - Correct answer Know how to (list the steps) view a microscope slide under all objective lenses, Including how to use the oil immersion objective. Know which objective should be used First to view a slide, and how to manipulate the amount of light that passes through the Slide. - Correct answer use the iris diaphragm to manipulate the light -begin with 4x lens -100x is for oil emersion Why did we use a nutrient gelatin deep? - Correct answer test for protein breakdown Why did we leave nutrient gelatin deep at room temperature, instead of using the incubator - Correct answer it breaks down because of the high temp. Of the incubator

On a Petri dish, know the different margins of a colony, and the different elevations of a Colony. - Correct answer page 26 On an agar slant, know the different growth patterns. - Correct answer page. 43 Algae microscope slide. - Correct answer Protozoa microscope slide. - Correct answer Fungi microscope slide. - Correct answer Gram stain microscope slide - Correct answer Acid fast stain microscope slide - Correct answer