MLT ASCP BOC Exam 2025–2026 (Medical Laboratory Technician) Accurate Real Exam Questions a, Exams of Nursing

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Which of the following species of Mycobacterium does NOT usually fluoresce on flurochrome stain? M. fortuitum. The optimum storage temperature for frozen RBCs is: -80 degrees Celsius. Antibodies involved in warm autoimmune hemolytic anemia are often associated with which blood group system? Rh. Infant: Anti-A - 4+; Anti-B - 0; Anti-D - 2+; Rh Control - 0; DAT - 0; AB Screen - NT Mother: Anti-A - 0; Anti-B - 0; Anti-D - +w(mf); Rh Control - 0; DAT - 0; AB Screen - 0 Which of the following is the most probable explanation for these results? Large fetomaternal hemorrhage. Mixed-field agglutination at the antihuman globulin phase of a crossmatch may be attributed to: An antibody such as anti-Sda. The use of deglycerolized RBCs would be most beneficial when transfusing a patient with: High frequency antibodies. A unit of Red Blood Cells is issued at 9:00 AM. At 9:10 AM the unit is returned to the Blood Bank. The container has NOT been entered, but the unit has NOT been refrigerated during this time span. The best course of action for the technician is to: Record the return and place the unit back into inventory.

Cryoprecipitated AHF, if maintained in the frozen state at -18 degrees Celsius or below has a shelf life of: 12 months. Which of the following is proper procedure for preparation of platelets from whole blood? Light spin followed by a hard spin. A 24-hour urine from a man who had no evidence of kidney impairment was sent to the laboratory for hormone determination. The volume was 600 mL, but there was some question as to the completeness of the 24-hour collection. The next step would be to: Report the hormone determination in mg per deciliter in case the specimen was incomplete. The following urine results were obtained on a 25-year-old female: Color: Amber Bacteria: Many Appearance: Cloudy WBC Cast: Many Specific Gravity: 1. WBC/HPF: 30- pH: 5. Protein: 1+ Glucose: Negative Blood: Small Pyelonephritis. A urine screening test for porphobilinogen is positive. The MOST likely disease state is: Acute prophyria attack. A condition in which erythrocyte protoporphyrin is increased is:

In the sweat test, the sweating stimulant is introduced to the skin by application of: An electric current. Oligoclonal bands are present on electrophoresis of concentrated CSF and also on concurrently tested serum of the same patient. The proper interpretation is: Nondiagnostic for multiple sclerosis. Which is associated with May-Hegglin anomaly? Dohle bodies and giant platelets. Elevations of the total white cell count above 12x19^9/uL is termed: Leukocytosis. The most appropriate screening test for hemoglobin S is: Dithionite solubility. Many microspherocytes, schistocytes and budding off of spherocytes can be seen on peripheral blood smears of patients with: Extensive burns. Muramidase (lysozyme) is present in: Granulocytes, monocytes and their precursors. The immunoglobin class typically found to be present in sAlivA, teArs, and other secretions is: IgA. In chronic active hepatitis, high titers of which of the following antibodies are seen? Anti-smooth muscle. ANA - Positive, 1: Complement - Decreased ASO - 50 Todd units RA - Positive. The above results could be seen in patients with:

Lupus erythematosus. From these test results it can be concluded that Patient #3 has: Patient #1 - HBSAg: Neg; anti-HBC IgM: Neg; anti-HAV IgM: Pos. Patient #2 - HBSAg: Pos; Anti-HBC IgM: Pos.; Anti-HAV IgM: Neg. Patient #3 - HBSAg: Neg.; Anti-HBC IgM: Pos.; Anti-HAV IGM: Neg. Acute hepatitis B. The stock cultures needed for quality control testing of OXIDASE PRODUCTION are: Escherichia coli/Pseudomonas aeruginosa. Which of the following results is typical of Campylobacter jejuni? Optimal growth at 42 degrees Celsius. Which sets of tests best differentiates Salmonella and Citrobacter species? KCN, malonate, beta-galactosidase, lysine decarboxylase. A beta-hemolytic streptococci which is bacitracin-resistant and CAMP-positive is: Group B. A TSI tube inoculated with an organism gave the following reactions: Alkaline Slant Acid Butt No H2S No Gas Produced Nonmotile. The organism is most likely: Shigella spp. Protozoan cysts are found in a wet mount of sediment from ethyl-acetate concentrated material. The cysts are without peripheral chromatin on the nuclear membrane. Each cyst has four nuclei and each nuclei has a large karyosome which appears as a refractive dot. These oval-shaped cysts are most likely.

Although ABO compatibility is preferred, ABO incompatible product may be administered when transfusing. Cryoprecipitated AHF. Genes of the major histocompatibility complex (MHC): Contribute to the coordination of cellular and humoral immunity. Coughing, cyanosis, and difficult breathing are symptoms of which of the following transfusion reactions? Circulatory overload. Optimum storage temperature for Whole Blood is: 4 degrees Celsius. Hives and itching are symptoms of which of the following transfusion reactions? Allergic. A patient has two separate urinalysis reports which contain the following data: Sp. Gravity: 1.004/1. pH: 5.5/7. Protein: Negative/Trace Glucose: Negative/Trace Blood: Negative/Trace. Microscopic: Rare epithelial cells/Occasional granular cast, Rare hyaline cast, Moderate epithelial cells. Which statement best explains these results? The protein, glucose, and microscopic of A are falsely-negative because of the specific gravity. Urinalysis performed on a 27-year-old woman yields the following results: Sp. Gravity: 1. pH: 5.

Protein: 2+ Glucose: Negative Ketones: Negative Bilirubin: Negative Blood: 3+ Nitrite: Negative Leukocytes: Positive Urobilinogen: 0.1 EU/dL

WBC/HPF: 20- RBC/HPF: 30-

Casts/LPF: Hyaline: 5- Red Cell: 2- Coarse Granular: 2- Waxy: 1-

Uric Acid Crystals: Moderate

Above data is consistent with: Chronic renal disease. 52-year-old man has urine glucose measurements performed as part of a three (3) hour glucose tolerance test. Best explanation for these findings is that the: Serum level must exceed the threshold level before reabsorption of glucose is exceeded.

Tay-Sachs disease. An adult diabetic with renal complications has the following results: Sodium: 133 mEq/L BUN: 84 mg/dL Glucose: 487 mg/dL Creatinine: 5mg/dL

Based off these results, the calculated serum osmolality is: 304 mOsm/kg. The formation of estriol in a pregnant woman is dependent on: Fetal and placental function. Arterial blood collection in a heparinized syringe but exposed to room air would cause which of the following changes in the specimen? pO2: Elevated. pCO2: Decreased. pH: Elevated. A 50-year-old woman who has been receiving busulfan for three years for CML becomes anemic. Lab tests reveal: Thrombocytopenia, Many peroxidase-negative blast cells in the peripheral blood, Bone marrow hypercellular in blast transformation, Markedly increased bone marrow TdT. Which complication is this patient most likely experiencing? Acute lymphocytic leukemia. Which is the most common cause of an abnormality in hemostasis? Quantitative abnormality of platelets. A leukocyte count and differential on a 40-year-old Caucasian male reveals...this data represents:

Absolute neutropenia. A patient has a normal PTT and a prolonged APTT using a kaolin activator. The APTT corrects to normal when the incubation is increased. These results suggest the patient has: Fletcher factor deficiency (prekallikrein.) Elevation of the TOTAL granulocyte count above 9.0 x 10^3/uL is termed: Absolute neutrophilic leukocytosis. The most appropriate screening test for hemoglobin H is: Heat instability test. PT - 20 sec. Thrombin time - 13 sec. APTT - 55 secs. APTT plus aged serum - Corrected. APTT plus absorbed plasma - Not corrected. Circulatory inhibitor - None present. Which coagulation factor is deficient? Factor X. Which laboratory procedure is most helpful in differentiating severe liver disease accompanying secondary fibrinolysis from disseminated intravascular coagulation? Factor VIII activity. Which laboratory finding is associated with Factor XIII deficiency? Clot solubility in a 5M urea solution. An automated leukocyte count is 22.5 x 10^3/uL. The differential reveals 200 normoblasts/100 leukocytes. What is the actual leukocyte count per microliter? 7,500/uL.