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A comprehensive overview of key concepts and procedures related to phlebotomy, including blood collection techniques, specimen handling, and common blood tests. It includes a series of questions and answers designed to prepare students for the phlebotomy certification exam.
Typology: Exams
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Capillary puncture 3rd or 4th finger for adults, heel s8ck for infants; lancet cut across ridges; hold the tube horizontal when filling. Don't sqeeze finger too 8ghtly or milk the finger. Wipe off 1st drop. The vein can collapse If you pull on the plunger of the syringe too hard or too quickly during a draw or you use too strong of a vaccum for the vein, this can happen... An8coagulant An agent used to preven blood cloJng and to keep the specimen whole blood Red top No addi8ve --- clot forms ----serum used for chemistries, electrolytes, thyroids (tsh, t4), hormone, liver, kidney, lipid stuidies, cmp, bmp. Tiger top Aka red/gray top: clot ac8vator addi8ve - clot forms - serum studies Lavender top Edta addi8ve - no clot - whole blood for: complete blood count (cbc), blood smear, blood banking (abo/rh), esr, hct, hmg,glyco alc *** must be filled sufficiently and inverted (gentle rocked) several 8mes *** Gold top Clot ac8vator addi8ve - clot forms - serum studies
Grey top Sodium fluoride/potassium oxalate - glucose, alcohol, Ys Light blue top Sodium citrate addi8ve - no clot - used for coagula8on studies (pt, pZ) *** tube must be filled to the proper level and inverted (gentle rocked) several 8mes *** Wipe away the first drop We do this to remove 8ssue juices, skin cells, alcohol, contaminates, avoid ac8va8ng cloJng process, avoid producing incorrect results, avoid dilu8ng specimen. The basilic, cephilic, and the median What veins are in the antecubical area? Hemoconcentra8on This is the increase of the cellular/solid elements in direct ra8o to plasma this can be caused by "pumping of the fist" and begins a_er the tourniquet is le_ on longer than 1 minute. Provodine/iodine Agent used on the skin to sterilize the area. Tourniquet Elas8c device used 8 restrict blood flow to distend veins for phelbotomy. Wing infusion set Aka buZerfly - for small or fragile veins or difficult veins Erythrocytes, leukocytes, and thrombocytes (platelets) The solid por8on of blood contains: Plasma The liquid por8on of whole blood; contains all cloJng factor Serum
The liquid por8on of cloZed blood; contains no cloJng factors. 3 - 4 inches abouve the puncture site. 1 minute. Where should the tourniquet be placed in regards to the draw site and how long should it be le_ on? Immediately remove the tourniquet and needle and stop the pa8ent from falling. Lower the pa8ents head and arms. Put something cold on the back of the neck. What should the ma do if the pa8ent begins to feel dizzy during the blood draw? Label tube, bandage pa8ent, document the draw. What the phelbo8mist to a_er the blood has been successfully draw and the needle has been properly disposed of: Complete blood count Wbc, rbc, hct, hemoglobin (hb), rbc indices ( mcv, mch, mchc), blood smears, wbc differen8al, platelet count are all considered what kind of test? Coagula8on studies Prothrombin 8me (pt) and par8al thromboplas8n 8me (pZ) are considered this kind of test... Lipid studies Cholesterol, triglycerides, hdl, ldl, are considered this kind of test... Chemistry panels Sodium, potassium, chloride, co2, glucose, bun, crea8nine, calcium, protein, albumin, globulin, bilirubin, alkaline phosphotase, ast, alt are all considered... Thyroid panel Tsh, t4 are considered... Mi studies Cpk, tropin, myo tests are all part of this...
Electrolytes Sodium, potassium, chloride, co2 are for this kind of test... Spun uncoagulated blood This type of blood sample has plasma on top, "buffy coat" white blood cells and platelets in the middle, red blood cells on the boZom. Spun cloZed blood This type of blood sample has serum on top, and clot on the boZom. Hemolysis Breaking apart of rbc's, causing serum or plasma to appear pink-red. This can be caused by a strong vaccum for the size of the vein, to small needle gauge, plunger pulled too quickly, pt has a hemoly8c disorder, shaking the tube, taking to long to complete draw. Blood smear For this you must clean the glass slide and use whole blood or capillary sample. Erythrocyte sedimenta8on rate (esr) This is a non-specific test for inflamma8on in the body. Use whole blood. Collected in lavender tube top. Soap and water When cleaning a site for a veinpuncture for alcohol test, use: Spinning a centrifuge You must always balance out, making sure euqally filled tubes are on each side when doing this: Hematocrit (hct) % of rbc's to total blood volume Bevel Angle end of needle that is inserted into vein/skin Hub
Holds needle Gauge Size of lumen or hole in needle, the smaller the number/guage, the larger the lumen. -- 18g: large size, not o_en used for veinpuncture 21g: used with vaccutainers 23g: used with buZerfly system or syringes 0-5 ml in capacity 25g: smallest gauge needle, o_en causes hemolysis of blood. Lumen Center of needle (hole) Safety needles Needles with a mechanical cap aZached for use a_er removal from pt. Blood cultures Need special training to draw these, so ma's usually don't draw them. Etoh Ethyl alcohol, when drawing for this test, use soap and water to draw to insure no contamina8on of specimen