Proteins - Techniques in Molecular Biology - Quiz, Exercises of Cellular and Molecular Biology

Download Proteins - Techniques in Molecular Biology - Quiz and more Exercises Cellular and Molecular Biology in PDF only on Docsity! Questions on Proteins 1. The figure below represents a Western Blot used to diagnose a patient with an HIV infection. The antibody recognizes several proteins that are present on the outer coat, or capsid, of the HIV virus. On the Western Blot below (left), lanes 1 and 2 represent a known HIV-positive and HIV-negative sample, respectively. Lanes A, B, and C represent samples from three individual patients. The criteria for establishing whether a patient is HIV-positive, negative, or indeterminate is listed in the table below: Interpret and explain the status of patients, A, B, and C from the data in the Western Blot. 2 Disulfide bonds play an important role in protein structure. Describe which amino acids are involved in forming these bonds, and how disulfide bonds contribute to specific levels of protein structure. 3. Why is SDS used in protein electrophoresis? 4. The protein bag-of-marbles (BAM) is known to be involved in regulating cell division during oogenesis in fruit flies. Genetic studies suggest that the BAM protein may interact with another fly protein called BGCN. Describe how you would test for protein interaction of these two proteins using the yeast two-hybrid system. Your answer should include how you would set up the experiment ( what constructs, proteins are involved). No bands present Negative Bands at either p31 OR p24 AND bands present at either gp160 OR gp120 Positive Bands present, but pattern does not meet criteria for positivity Indeterminate Docsity.com