Download Zoology 211: Spring '92 Exam on Enzyme Kinetics & Muscle Physiology - Prof. William J. Hig and more Exams Cell Biology in PDF only on Docsity! Zoology 211: Spring, 1992 ___________________ Higgins & Colombini Name (printed, please) Examination HI Lab section # or meeting time Directions: 1. Please PRINT your name on each page of the examination NOW! Place your lab section information on the first page (above). You should have 7 pages. 2. Read through the questions with Dr. Know-it-all before you begin. 3. Please complete the exam in ink if you wish the right to a regrade. 4. Be clear, concise, complete, and brief. Confine your answers to the spaces provided. GOOD LUCK! SCOREBOARD Page Higgins's Points Visitor's Score 2 24 12 4 & 5(a) 24 5(b), 6, & 7 20 5(b), 6, & 7 20 EXAM TOTAL1 = 100 points ________ points The lecture "contest" stands at Visitors 4, Higgins 0. But remember two relevant quotes: "It ain't over 'til it's over!" and "I'll be back!" #1 Name Dr. Know-it-all, that noted enzymologist and part-time ZOOL 211 lecturer, has recorded and plotted the following data based on the conversion of Substrate S to Product P by the enzyme Terpase. [SU VireacUon 1 mM 0.2 umoles/min 2 0.4 3 0.6 4 0.8 5 0.8 10 0.8 y v '-f-' i ) *- a. On graph A, label the Vmax and km . b. Please give the numerical values of points #1 and #2 in graph B. (Forget the units!) #1 = #2 = c. On graph A, draw a curve representing noncompetitive inhibition of the reaction, d. On graph B, draw a curve representing competitive inhibition of the reaction, e. For this reaction, what does the enzyme Terpase do to the: - equilibrium constant (K^)? ____________________________ - Free energy (delta G)? ___________________________ - Ea (Activation energy)? _________________________ - Rate of the reaction? __________________________ f. What do you know about the free energy (delta G) for this reaction? ______ g. If the conversion of Substrate S to Product P is an endothermic reaction, what must be happening to allow it to proceed spontaneously? Answer: NAME #13 continued: e. enthalpy of reaction is negative or positive? ________________ f. oxygen is reduced or oxidized? g. is endothermic or exothermic? #14. Why do yeast conduct fermentation reactions? NOTE: You must complete any two (2) (AND ONLY TWO} of the following four questions (# 15, 16 ,11, & 18). Please mink carefully, organize your answers, and be as complete as possible IN THE SPACE PROVIDED! #15. In A draw two amino acids below (use an "R" for the side chain) and then in B draw these two amino acids linktjd by a peptide bond: A: !: Now briefly describe the factors which determine the primary through quaternary structures of a protein. Draw the structure of the Intermediate Filament protein vimentin and label the relevant parts of it as examples of secondary and quaternary structures. Label the domains. Name #16. In the space provided below and without pictures, define/describe "chemiosmotic synthesis of ATP". #17. Draw a cilium in cross-section. Label as many structures and components as possible. Then in fewer than 50 words, describe how it bends. Name #18. Consider the protein myosin. Draw its structure. Label each domain and subunit. Describe how myosin molecules fit together to form a "thick filament" in muscle. Finally, draw or describe the movements of the head region of myosin during contraction. Be sure to include all other molecules that are involved in this movement.
