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NAME. Circle Wh SECOND MIDTERM EXAM BE 463/563 Fail, 2005 ~~ ay ich Class You Are In: BC463 BC563 Mary Paule Department of Biochemistry and Molecular Biology (20 PTS) 1. On the following list of transcription factors, please match the function of the factor from the left hand column with its name by entering the number of the factor in the space provided on the left of the description. In the space provided to the right of the factor names, indicate whether the numbered factor listed in the middle column is recruited to the developing preinitiation complex by directly binding to DNA on its own (D), by protein-protein interaction with a previously bound factor on the promoter (P-P) or is recruited along with RNA polymerase, either in a preformed complex on the polymerase or by joining polymerase and any of its associated factors later (Pol). For example, the answer to a. and to 1. are filled in (1 point each). ’ a _ 1 An example of an architectural transcription factor. 6. __10____A transcription factor involved primarily in stimulating elongation. e414 A transcription factor whose smallest subunit is required for RNA polymerase II recruitment and stimulates elongation. d. _ 9 A transcription factor that recruits the DNA helicase in polymerase I transcription. e.__6___ The assembly transcription factor used by all ICR-containing RNA polymerase IH genes. fi _7 A transcription factor that has DNA helicase activity. g.__12___ One of the TBP-containing fundamental transcription factors whose roie is in transcription by RNA polymerase II. bh 2 The key growth-regulated transcription factor for ribosomal RNA transcription. i,_ 4 A transcription factor only needed for transcription of RNA polymerase I Type I- promoter-containing genes. j. 3. One of the TBP-containing fundamental transcription factors whose role is to recruit RNA polymerase I to the promoter. Page 1 of 6 Factor Name 1. UBF 2. TIFIA 3, TIF-IB 4. TEINA 5. TFIIB 6. TFHIC 7. TFOH 8.TFIB - 9. TFIE 10. TFIS 11. TFIF 12, TFOD Recruited how?