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EXAM 2 - Spring 2010
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/ The exam is worth 300 points, 30% of the possible course points
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rotein Structure and Function
E01 (20 points) Protein structure is dictated by the amino acid sequence. Should a
protein, in which the order of all amino acids is reversed, (ABC->CBA) have the same
structure as the original protein? Explain with supporting information.
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Prade et. al. Cell and Molecular Biology MICR3033 Spring 2010
06 (20 points) Replication proceeds at replication forks in E. coli in a semi-
discontinuous manner.
A- what does semi-discontinuous mean?
B - What property of DNA polymerases results in the need for seri-discontinuous
synthesis?
C - What is an Okazaki fragment?
D - What is the "priming problem" for synthesis of an Okazaki fragment?
E - Why is an RNA primer a good solution to the priming problem?
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Prade et. al. Cell and Molecular Biology MICR3033 Spring 2010
E07 (20 points) What are primases used for in replication? What do they synthesize?
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a \ Prade et, al. Cell and Molecular Biology MICR3033 Spring 2010
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E09 (20 points) RNA polymerase transcribes from both strands. How is than the
transcript organized and the genetic code interpreted?
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pg. 6