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Biochemistry: Glycolysis, Appunti di Inglese

Breve lezione in inglese di Biochimica sulla glicolisi, partendo da una descrizione dettagliata si arriva alla spiegaizone passo passo degli steps della glicolisi.

Tipologia: Appunti

2022/2023

In vendita dal 13/07/2023

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ACADEMIC SPEECH - BIOCHEMISTRY
Cellular respiration can be broken down into 3 phases.
The first one is glycolysis, which is the breakdown of glucose. This can occur with or
without oxygen. If we don’t have oxygen, then we go over to fermentation, producing
lactic acid or ethanol. But if we have oxygen then we can proceed with the Krebs
cycle and then we proceed to the electron transport chain.
The whole purpose of cellular respiration is to generate energy in the shape of ATPs,
or adenosine triphosphate composed of adenine + ribose + 3 phosphoryl groups.
This groups have high energy bonds. If we break one of this bonds, the electron are
free and are going to release energy. So one of the phosphate grous is going to
popp off and we’ll have ADP, adenosindiphosphate (with just 2 phosphoryl groups.
Glycolysis is the first step in the breakdown of glucose to extract energy for cellular
metabolism. Glycolysis consists of an energy-requiring phase followed by an
energy-releasing phase.
What is glycolysis?
Glycolysis is a series of reactions that extract energy from glucose by splitting it into
two three-carbon molecules called pyruvates. Glycolysis is an ancient metabolic
pathway, meaning that it evolved long ago, and it is found in the great majority of
organisms alive today
Highlights of glycolysis
Glycolysis has ten steps, we’re gonna firstly highlight the key steps and principles
without tracing the fate of every single atom.
Glycolysis takes place in the cytosol of a cell, and it can be broken down into two
main phases: the energy-requiring phase and the energy-releasing phase.
In the energy-requiring phase, the starting molecule of glucose gets
rearranged, and two phosphate groups are attached to it. The phosphate
groups make the modified sugar, now called fructose-1,6-bisphosphate,
unstable, allowing it to split in half and form two phosphate-bearing
three-carbon sugars. Because the phosphates used in these steps come from
ATP, two ATP molecules get used up. The three-carbon sugars formed
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ACADEMIC SPEECH - BIOCHEMISTRY

Cellular respiration can be broken down into 3 phases. The first one is glycolysis, which is the breakdown of glucose. This can occur with or without oxygen. If we don’t have oxygen, then we go over to fermentation, producing lactic acid or ethanol. But if we have oxygen then we can proceed with the Krebs cycle and then we proceed to the electron transport chain. The whole purpose of cellular respiration is to generate energy in the shape of ATPs, or adenosine triphosphate composed of adenine + ribose + 3 phosphoryl groups. This groups have high energy bonds. If we break one of this bonds, the electron are free and are going to release energy. So one of the phosphate grous is going to popp off and we’ll have ADP, adenosindiphosphate (with just 2 phosphoryl groups. Glycolysis is the first step in the breakdown of glucose to extract energy for cellular metabolism. Glycolysis consists of an energy-requiring phase followed by an energy-releasing phase.

What is glycolysis?

Glycolysis is a series of reactions that extract energy from glucose by splitting it into two three-carbon molecules called pyruvates. Glycolysis is an ancient metabolic pathway, meaning that it evolved long ago, and it is found in the great majority of organisms alive today

Highlights of glycolysis

Glycolysis has ten steps, we’re gonna firstly highlight the key steps and principles without tracing the fate of every single atom. Glycolysis takes place in the cytosol of a cell, and it can be broken down into two main phases: the energy-requiring phase and the energy-releasing phase. ● In the energy-requiring phase, the starting molecule of glucose gets rearranged, and two phosphate groups are attached to it. The phosphate groups make the modified sugar, now called fructose-1,6-bisphosphate, unstable, allowing it to split in half and form two phosphate-bearing three-carbon sugars. Because the phosphates used in these steps come from ATP, two ATP molecules get used up. The three-carbon sugars formed

different from each other. Only one—glyceraldehyde-3-phosphate—can enter the following step. However, the unfavorable sugar, DHAP, can be easily converted into the favorable one, so both finish the pathway in the end ● In the energy-releasing phase, each three-carbon sugar is converted into another three-carbon molecule, pyruvate, through a series of reactions. In these reactions, two ATP molecules and one NADH molecule are made. Because this phase takes place twice, once for each of the two three-carbon sugars, it makes four ATP and two NADH. Overall, glycolysis converts one six-carbon molecule of glucose into two three-carbon molecules of pyruvate. The net products of this process are two molecules of ATP (4 ATP produced - 2 ATP used up) and two molecules of NADH. .

Detailed steps: Energy-requiring phase

We’ve already seen what happens on a broad level during the energy-requiring phase of glycolysis. Two ATPs are spent to form an unstable sugar with two phosphate groups, which then splits to form two three-carbon molecules that are isomers of each other. Next, we’ll look at the individual steps in greater detail. Each step is catalyzed by its own specific enzyme, whose name is indicated below the reaction arrow in the diagram below.

Step 1. A phosphate group is transferred from ATP to glucose, making

glucose-6-phosphate. Glucose-6-phosphate is more reactive than glucose, and the addition of the phosphate also traps glucose inside the cell since glucose with a phosphate can’t readily cross the membrane.

  1. 1,3-bisphosphoglycerate is converted to 3-phosphoglycerate by phosphoglycerate kinase. This step converts an ADP to an ATP.
  2. 3-phosphoglycerate is converted to 2-phosphoglycerate by phosphoglycerate mutase.
  3. 2-phosphoglycerate is converted to phosphoenolpyruvate (PEP) by enolase. This reaction releases a water molecule.
  4. Phosphoenolpyruvate (PEP) is converted to pyruvate by pyruvate kinase. An ADP is converted to an ATP in this reaction. Step 6. Two half reactions occur simultaneously: 1) Glyceraldehyde-3-phosphate (one of the three-carbon sugars formed in the initial phase) is oxidized, and 2) NAD+ is reduced to NADH and H+. The overall reaction is exergonic, releasing energy that is then used to phosphorylate the molecule, forming 1,3-bisphosphoglycerate. Step 7. 1,3-bisphosphoglycerate donates one of its phosphate groups to ADP, making a molecule of ATP and turning into 3-phosphoglycerate in the process. Step 8. 3-phosphoglycerate is converted into its isomer, 2-phosphoglycerate. Step 9. 2-phosphoglycerate loses a molecule of water, becoming phosphoenolpyruvate (PEP). PEP is an unstable molecule, poised to lose its phosphate group in the final step of glycolysis. Step 10. PEP readily donates its phosphate group to ADP, making a second molecule of ATP. As it loses its phosphate, PEP is converted to pyruvate, the end product of glycolysis. What happens to pyruvate and NADH? At the end of glycolysis, we’re left with two ATP, two NADH, and two pyruvate molecules. If oxygen is available, the pyruvate can be broken down (oxidized) all the way to carbon dioxide in cellular respiration, making many molecules of ATP. What happens to the NADH? It can't just sit around in the cell. Glycolysis needs NAD+ to accept electrons as part of a specific reaction. All cells need a way to turn NADH back into NAD+ to keep glycolysis going. There are two basic ways of accomplishing this. When oxygen is present, NADH can

pass its electrons into the electron transport chain, regenerating NAD+ for use in glycolysis. (Added bonus: some ATP gets made!) When oxygen is absent, cells may use simpler pathways to regenerate NAD+. In these pathways, NADH donates its electrons to an acceptor molecule in a reaction that doesn’t make ATP but does regenerate NAD+ so glycolysis can continue. This process is called fermentation.