Macromolecule Web Quest: Identifying Carbohydrates, Proteins, and Fats in Foods, Schemes and Mind Maps of Chemistry

Instructions for a web-based lab activity aimed at helping students identify different macromolecules, specifically carbohydrates, proteins, and fats, in various foods. The activity includes instructions for using online tests and interpreting results to determine the presence of these macromolecules in foods.

Typology: Schemes and Mind Maps

2021/2022

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UNIT
7
ACTIVITY
Macromolecule Web Quest
Purpose. Now that we know the purpose of macromolecules, we need to answer the question
of where they come from. Plants are able to make macromolecules on their own. However,
we learned that humans are unable to produce macromolecules on their own. In order to
obtain macromolecules, or the parts to build them, humans must eat. Therefore, we can
conclude that macromolecules are found in foods. This web quest is in place of a lab in which
we would explore our food and what macromolecules it contains.
Part One: Introduction
In part one, you will learn how to identify the different macromolecules through a variety of
tests.
1. Login and open your web browser.
2. Type in the following address: http://bit.ly/7bmacrolab
3. Read the instructions in red and blue.
a. What macromolecules will you be testing in this online lab?
_________________________________________________________
4. Click on carbohydrates.
a. Look at the diagrams. According to the diagrams, what elements are found in
carbohydrates? ___________________________________________
5. Read the information in red.
a. How will the Benedict solution tell you there is sugar present?
___________________________________________________
b. What type of sugar does Benedict solution test for?
___________________________________________________
6. Click on the link “click here to fill a test tube with 2mL of water.” When the next page
loads, click on “click here to add 2mL of Benedict’s reagent to the water.”
7. Benedict’s reagent must be heated in order for the test to work. Click on the picture of the
beaker on the hot plate.
a. What color is the water in the test tube? ________________________
8. Click on the link that says “click here” next to the picture of the glucose solution. When
you do this, you are adding simple sugars (monosaccharides) to the test tube.
a. What is the color of the solution in the test tube? ____________________
9. Click on “click here to continue.” Read the information in red.
a. The negative result (no sugar present) has a _____________ color.
b. The positive result (sugar present) has a ________________ color.
Name!Date!Per
Unit 7 | Activity | Macromolecule Web Quest"Page 1
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UNIT

ACTIVITY Macromolecule Web Quest Purpose. Now that we know the purpose of macromolecules, we need to answer the question of where they come from. Plants are able to make macromolecules on their own. However, we learned that humans are unable to produce macromolecules on their own. In order to obtain macromolecules, or the parts to build them, humans must eat. Therefore, we can conclude that macromolecules are found in foods. This web quest is in place of a lab in which we would explore our food and what macromolecules it contains. Part One: Introduction In part one, you will learn how to identify the different macromolecules through a variety of tests.

  1. Login and open your web browser.
  2. Type in the following address: http://bit.ly/7bmacrolab
  3. Read the instructions in red and blue. a. What macromolecules will you be testing in this online lab? _________________________________________________________
  4. Click on carbohydrates. a. Look at the diagrams. According to the diagrams, what elements are found in carbohydrates? ___________________________________________
  5. Read the information in red. a. How will the Benedict solution tell you there is sugar present? ___________________________________________________ b. What type of sugar does Benedict solution test for? ___________________________________________________
  6. Click on the link “click here to fill a test tube with 2mL of water.” When the next page loads, click on “click here to add 2mL of Benedict’s reagent to the water.”
  7. Benedict’s reagent must be heated in order for the test to work. Click on the picture of the beaker on the hot plate. a. What color is the water in the test tube? ________________________
  8. Click on the link that says “click here” next to the picture of the glucose solution. When you do this, you are adding simple sugars (monosaccharides) to the test tube. a. What is the color of the solution in the test tube? ____________________
  9. Click on “click here to continue.” Read the information in red. a. The negative result (no sugar present) has a _____________ color. b. The positive result (sugar present) has a ________________ color. Name Date Per Unit 7 | Activity | Macromolecule Web Quest Page 1

10.Click on “click here to continue.” Now you will be testing if a solution contains starch. Iodine is used because it changes color when it comes in contact with a starch. We use a different substance to test for starches because starches are long chains of simple sugars connected together. Benedict’s solution can only detect simple sugars, not starches. Click on “click here for results.” a. What is the color of the solution of water and iodine? ____________________ 11.Now you will add the starch solution to the container. Click on the Argo corn starch box. a. What is the color of the solution now? ______________________ 12.How do you know if a starch is present in the solution by adding iodine? a. The negative result (no starch present) has a ______________ color. b. The positive result (starch present) has a _______________ color.

  1. Great! You now know how to test for sugar and starches. In other words, you know how to determine if a substance has carbohydrates in it. Click on “click here to continue.” Then click on proteins. a. Look at the diagram. What elements are present in proteins? (Ignore R) __________________________________________ 14.Read the information in red. a. How will the biuret reagent tell you there is a protein present?

15.Click on “click here to fill a test tube with 2mL of water.” Read the information to verify your answer for #14a. Click on “click here to add 2 m: of Biuret...” a. What is the color of the solution? ____________________ 16.Click on “click here to continue.” Now we will add the protein solution to the test tube to see what happens. Click on the picture of the protein solution. a. What is the color of the solution now? _____________________ 17.Click on “click here to continue.” How do you know if protein is present in a solution by adding biuret reagent? a. The negative result (no protein present) has a _______________ color. b. The positive result (portein present) has a _________________ color. 18.Great! You now know how to test for proteins. Click on “click here to continue.” Then, click on “ Fats ” at the bottom of the page. a. Look at the diagram. Which elements are present in fats (lipids)? _____________________________________________________ 19.Read the information in red. a. How will the Sudan IV tell you there is a fat (lipid) present? _________________________________________________

Table A: Color test result (indicate the color you saw)

Food Sugar Starch Protein Fat

Potatoes Light Blue Dark Brown Clear Clear

Orange Juice

Almonds

Eggs

Salmon

Milk

Table B: Positive or negative result (indicate with a YES if it is a positive result or NO if it is a negative result).

Food Sugar Starch Protein Fat

Potatoes No Yes No No

Orange Juice

Almonds

Eggs

Salmon

Milk

Part Three: Results Under each macromolecule, list which foods are a good source for that macromolecule. Carbohydrates will contain responses for both sugar and starch.

Carbohydrates Proteins Lipids (Fats)