Prepare for your exams
Study with the several resources on Docsity
Earn points to download
Earn points by helping other students or get them with a premium plan
Guidelines and tips
Prepare for your exams
Study with the several resources on Docsity
Earn points to download
Earn points by helping other students or get them with a premium plan
Bacteria Expression: E. coli and Bacillus subtilis as Protein Expression Systems, Study Guides, Projects, Research of Medical Sciences
The use of e. Coli and bacillus subtilis as protein expression systems in laboratories and industries. E. Coli, a gram-negative bacterium commonly found in the lower intestine, is the most widely used system due to its rapid growth, high yield, and ease of manipulation. B. Subtilis, a gram-positive bacterium commonly found in soil and the gastrointestinal tract, is an increasingly popular alternative due to its fda designation as gras, lack of endotoxin concern, and codon optimization. Profacgen offers custom production services for both systems, ensuring high-quality and high-level production of target proteins.
Typology: Study Guides, Projects, Research
2017/2018
1 / 2
This page cannot be seen from the preview
Don't miss anything!
Related documents
Partial preview of the text
Download Bacteria Expression: E. coli and Bacillus subtilis as Protein Expression Systems and more Study Guides, Projects, Research Medical Sciences in PDF only on Docsity!
Bacteria Expression
Bacteria are the most widely used protein expression systems for their rapid growth, high yield, ease of manipulation and scale-up. The most widely used bacteria host is of course Escherichia coli. E. coli is the most established tool and is almost always the first choice if applicable. Profacgen offers various E. coli strains and expression vectors to seek for high-quality and high-level production of your target proteins.
Features
One-stop service Time-efficient & Cost-effective High flexibility for custom production
Escherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms. In labs, they can be grown and cultured easily and inexpensively, and have been intensively investigated for over 60 years.
Another bacterium, Bacillus subtilis , is an increasingly popular protein expression host. It is a FDA designated Generally Regarded As Safe (GRAS) organism. B.subtilis is a Gram-positive bacterium, which makes this system free of endotoxin concern. It also has much less codon bias than E. coli. Moreover, it secretes protein directly into the culture media, which highly simplifies subsequent
protein purification steps. All the above features make it an attractive system for the production of soluble and properly folded recombinant proteins at both laboratory and industrial levels.
Bacillus subtilis is a Gram-positive, rod-shaped bacterium that is commonly found in soil and the gastrointestinal tract of ruminants and humans. They are well known for their contributions to agricultural, medical and food biotechnology and production of recombinant proteins.
Profacgen provides you with an ultimate solution of bacteria production. Our service can be tailored according to your specialized requirements (codon optimization, tag selection, purity, endotoxin removal, protein refolding etc.). Our expertise guarantees high protein quality, short turnaround time, and the best price in the market!
See more details on the principle and protocol of baceria expression system on our website. See more...