Bacteria Growth Experiment: Hypothesis and Procedure, Lecture notes of Medicine

A lab experiment for university students to investigate how bacteria grow, their visibility, and the areas with the most and least bacteria. The students are required to make hypotheses, perform the experiment using petri dishes and agar, and record observations for several days. Safety precautions are also provided.

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2021/2022

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Student Name: _____________________________ Date: ____________________
Activity Sheet 1
BACTERIA TAKE OVER
Problem:
How does bacteria grow? Can bacteria be visible? What areas have the most and least bacteria?
Background:
Germs are EVERYWHERE!!! This is something that you most likely have heard. Quite often
illnesses and diseases can be caused by the transmittance of some sort of microorganism. These
different microorganisms
could vary from some sort of virus
, fungi
, or bacteria
. Bacteria in particular
are single celled microbes. The cell structure is simpler than that of other organisms as there is no
nucleus
or membrane
bound organelles. Instead their control centre containing the genetic information
is contained in a single loop of DNA
.These little invaders use the nutrient rich and warm human body to
prosper and to replicate themselves (Alberts, Johnson, & Lewis). An example of a harmful bacteria is E
Coli. When E Coli is found in food or water it can be harmful to our bodies.
Hypothesis:
#1 How does Bacteria Grow?
I believe that…
________________________________________________________________________
Because,
________________________________________________________________________
#2 Can bacteria be visible?
I believe that…
________________________________________________________________________
Because,
________________________________________________________________________
#3 What areas have the most and least bacteria?
I believe that…
________________________________________________________________________
Because,
________________________________________________________________________
Materials:
- petri dishes (1-2 per group)
- Nutrient agar powder (or agar powder and
beef boulion cube)
- heater or incubator
- cotton swabs
- hand sanitizer
- marker for labeling
- liquid bleach
- lab safety gloves
- miscellaneous metals
-1–
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Activity Sheet 1

BACTERIA TAKE OVER

Problem: How does bacteria grow? Can bacteria be visible? What areas have the most and least bacteria? Background: Germs are EVERYWHERE!!! This is something that you most likely have heard. Quite often illnesses and diseases can be caused by the transmittance of some sort of microorganism. These different microorganisms could vary from some sort of virus , fungi , or bacteria. Bacteria in particular are single celled microbes. The cell structure is simpler than that of other organisms as there is no nucleus or membrane bound organelles. Instead their control centre containing the genetic information is contained in a single loop of DNA .These little invaders use the nutrient rich and warm human body to prosper and to replicate themselves (Alberts, Johnson, & Lewis). An example of a harmful bacteria is E Coli. When E Coli is found in food or water it can be harmful to our bodies.

Hypothesis: #1 How does Bacteria Grow? I believe that…


Because,


#2 Can bacteria be visible? I believe that…


Because,


#3 What areas have the most and least bacteria? I believe that…


Because,


Materials:

  • petri dishes (1-2 per group)
  • Nutrient agar powder (or agar powder and beef boulion cube)
  • heater or incubator
  • cotton swabs
    • hand sanitizer
    • marker for labeling
    • liquid bleach
    • lab safety gloves
    • miscellaneous metals

Safety:

  • Wearing lab safety gloves, goggles, and an apron or lab coat
  • Make sure that bacteria is dead before disposing of it by washing it down the sink. This can be done by using bleach.
  • Make sure that all bacteria is contained and that hands are always washed after working with samples.

Procedure:

  1. Aquire 1-2 agar plates. Plate cover should not be removed until instructed to do so.
  2. Take these petri dishes and turn upside down. Label each section with the area you indent to introduce bacteria for. An example of a plate is to the right. Make sure that one section of your plate is a “control” section where no bacteria will be plated (the areas to take bacteria from to the right are only suggestions. You should test the areas that you and your group members hypothesize in your third hypothesis on the first page.
  3. Use a cotton swab and wipe it along a surface that your group has chosen to test thoroughly. ** Note: The next few steps(4-6) should be done quickly in order to ensure the least amount of contamination**
  4. Lift the lid off of one of your petri dishes. (Do not set down on the countertop. You will replace as soon as you are done introducing the bacteria)
  5. Using the cotton swab that you swiped your surface with, swipe the appropriate section of the dish without breaking the agar. A diagram is given below of a petri dish section with how to introduce bacteria into your plate in order to gradually decrease the concentration of the bacteria on your plate.
  6. Quickly replace the lid of your petri dish.

Data Sheet

Day 1:

Diagram-

Observations-

Day 2:

Diagram-

Observations-

Day 3:

Diagram-

Observations-

Day 4:

Diagram-

Observations-

Day 5:

Diagram-

Observations-

Day 6:

Diagram-

Observations-

Discussion Questions:

  1. How extensive was the growth of the “control” section of your petri dishes? Did this surprise you? Explain.
  2. What area has the most and least bacteria based off of the classroom data? Explain why you think this might be.
  3. Was their bacteria growth in the “control” section of your petri dish?
  4. What you you believe the ideal “microbiome” is?
  5. Does all the bacteria look the same? Did the bacteria all grow in a certian pattern? If yes what are the main characteristics of it? If no what are some of the differing characteristics of it?
  6. Do you believe that you have grown different varieties or species of bacteria? … yes bacteria are living things!
  7. What do you believe the purpose of antibacterial soap and hand sanitizers are? Do you think they are important? Explain why.