Mantoux Tuberculin Skin Test: Administration, Reaction, and Measuring Induration, Slides of Immunology

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Diagnosis of Latent TB Infection (LTBI)

Mantoux Tuberculin Skin Test

Administering the Test

Mantoux Tuberculin Skin Test (1)

• TST is administered by injection
• Tuberculin is made from proteins derived from inactive tubercle bacilli
• Most people who have TB infection will have a reaction at injection site Syringe being filled with 0.1 ml of liquidtuberculin

Mantoux Tuberculin Skin Test (3)

• Forearm should be examined within 48 - 72 hours by HCW
• Reaction is an area of induration (swelling) around injection site - Induration is measured in millimeters - Erythema (redness) is not measured Only the induration is measured

Multiple-Puncture Test

• In the past, multiple-puncture tests (tine tests) were a popular skin testing method for TB
• No longer recommended
  • Amount of tuberculin that enters skin cannot be measured
• Mantoux TST is preferred TB skin test method because amount of tuberculin can always be measured

Mantoux Tuberculin Skin Test Study

Question 3.

How is the Mantoux TST given? (pg. 11)

The TST is given by a needle and syringe to inject 0.1 ml of 5 tuberculin units of liquid tuberculin between the layers of the skin, usually on the forearm.

Mantoux Tuberculin Skin Test

Study Question 3.

With the TST, when is the patient’s arm examined? (pg. 12)

The patient’s arm is examined by a health care worker, 48 – 72 hours after tuberculin is injected.

Mantoux Tuberculin Skin Test Study

Question 3.

Why is the Mantoux TST preferable to multiple puncture tests? (pg. 12)

Mantoux TST is preferable because it is more accurate and the amount of tuberculin can always be measured.