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ag =< i A | 1 flnatgbiea Cementrg nei Sr _ | she = Sewerce of obtaining , processing + Commernicationa, I tmformatish | aboeet Zen 9 Com postion & 0 Arete of matley ST gels tw wrt setemce | of determi SS ning what, mOtkeY ib a ee Oo | 9 imeludes tn stiles —& ses imate] “a nty & methods to Keporate _» dofemtif y & ‘yy quand y matter’ U Bo ge IC cteak EF the amalsis of matter t 4 ‘ a 7 4 Quattative Quantative NG : i) Cvautanetrte, Clits 4 ji). Velumetrte analytes. | in 27 | Betsorftion = 9t “ss Qa Auyfate feenomenon where the particles ( solid , U gas ) ove attacrh the [top Layer surface of material - IC I es aw Frocetsv tract! involves he SAAT PSP op a aE U Bb Positive... _ ad CE Scanned with OKEN Scanner CE Scanned with OKEN Scanner DATE:_L_|__ 2,220 ORs pu ace emivar, Comnesuuanithy YC tue U surfastin Cope, tuts oe or stick U hone aw Combibr ex, Le i Arede' § vetain tte rmolentay UV specs 6 @ | Comes im Com tach’ = Pictsorptcon bon exothorence _ frveess hes) te surface emevary is | veteasecf ms Ue fartietely) get 00 a ttatteof tthe ' Ate ee v <> | Tudes of aahror$tion. ff I fe Plg sical _aolbord tion Chemical adlserftion { orl Plagsisorptton Or Chemesor put 4 i) dere Utne Tacmsovbate |i) were the acthorbate imtevaets % adsorbent, +,| interacts E adsorbent by, _|lweak — Vamderwatts forces | chemecat tonal forms j — (Covalent, a4 well lbomte) , DOC 6 & omuutelagereef |i) Jt 6 a serate i process kK Shot Cayered proces by sheetfc im nature , | spbeific Uh nature, ie "te Utahes flaw alt |ocewd af ther speerfie ovey thw ad sorbent: vty Centres 9 om the | ccbtedrert” va) || Weve tee emthatpy of |i) Here the emthatpy aolsorption By Vevey V baw % vO". nigh (- Yoo (Gna) i (ad- yo ef mot) ’ wl No actiiation energ, |W) Mere tee activation is yequered. og eatiTi 4 meecfed, yl ot 6 U reversible ty |v) 90" 3 brrwersthle imereasing fermp- & deeveasing ehuree i$ q tt Be Positive... ( DATE: LI \ a “TI ~ q namely thy _stationany. phase. a a | dase She stationod, / poase CQx be Qa : : ; q hothal ov tiguiol _, 7 asthe tle mothe ' frase ts heguid ov gad wham) he 4 shatlonane, phase Mu boliek tue _—hehective 4 aistribeltion | is based on + aclkorptlon. whem 4 i is a Uguid , tow selective | éstribution 4 partition’ Factoys atpecting Kepayation pees Moteulay. ll chavacteristics veearterl Lo a lua on one? 3 actor blion ; partition 4 affinity or I de lgeremtes ermong their an) weigntty 4 U ; Vos ComporentS of thy ombbtdive, story) tomer 4 a in | ew Ustationany phrase. xO mov showy én he suyhthhom ; ubile OTL LS, hans! rerpidileg into. moblte prose & eave tae 9 butte fasten: ae She tee of 9 interadltion Af stationary phase» OT mopte prone. & toe seubstambes | Combined in thy mink is hw botic Component effective tm Keparateon of mmol eLeees from W eath Otho I Sy i Intevattion 0 fas Un moleetles are plea &. tnvolve weet chemecal ih pbractions ‘tihe a aifole imteractlon & u- bone danation lie tee they aolere to the |) statlonany fomse. Dering the movement of som a behar VeAUL is formesh toes thy repeated" desreptton O & acl nptiion 7 he cktreetin of L obhbe, migration bus, Chyroma- FY ( Be Positive. CE Scanned with OKEN Scanner DATE:__L_/— Cp LLoren uses fpiase. egucllibrim “Piola a! aporadl —froteing | mastoid ‘or Amo opt eeuelos lin compere s_ * amiatinres howd on thats interaction EO *s prationanry pede, & mile pees = | id eT @ +» | Clanctcation eof Chromatapaphy: a | hh V-tTd ” HL Based on ead. 3 , Column Chroma tngprarlery: ny Planar Chromatnene blu! ro Paper _Chromaxtogra ply, , VTC. Yee mam, es. ] oa iy 2). Base on mile prove. At | Gas brome tog ep ay eg utof Clmomactetn a bk, = UPLe ii v ( a Super creticat fusid Claro: “ 3).|| Basecl on Aaparialion mechamttm,. wnt dL Detter ftien —“ebmomaLepyapiry yi Pantitior » U ~ + | Dfenites : a J. 40 exibition Z ~ 7 a + ||\Jeh _ exehamae 4. =A d y “5 || Aelsorption _ Chnomatogralass . » I involves Yael VU sefanation of a ey chemecal omitture, hasecl om the interaction aj of the acktybate EE the adsorbent,» In ais an U Becee Ad the minture aes or Ligubel Gety a) ; sefana.tes! om the ea V beet thay actdbyb £ &) Be Positive... CE Scanned with OKEN Scanner "9 ™ DATE:__/_/__ Ss % ts am rampte of Sotuol-_Laquial | Chrometogapir | 9¢ lis used tn, mpanate -¥ month, Ul ¢ the mon - volatile Combonen th of 4 tre | minture She stationoye, droge “ i Ss) Qa te pate mace uf of! a lee, ~ yeactive 4S folic coated é a thin | Losey __ of SS astberbent, motertal & the Gwite | paase => ha solvent. er a eminture of solver B5: 2» The separation of Combourets sl clue ito x the cbifferemees bp theirs Attraction to he a) Atationan, S/ 4yase g been, of the — A erences ~ im Soblubetiti,| in tne Vmmebtle — poose W tt Netlerent” Y Compounds im a amply mentire 4 trovdl at hiftent rates tue! te Liltpanee _ bn thin partition Coeffcrtent ev WW a Aitty hubtor j coef fucienty W/ the yatlo of m Conc. of a Co omddne & Qa mitture ! of vi tivo | emmenerbte | solvents at equilib, “~ whix ati is a Combaneson of U the selube- J tities of tw —_lutes im these tun draros). 4 The rebanctation _factov __ quamhifios tha heruutt § U C 0 _ Rp 2 otistiance travelled! by substance 2 af distameg, Crowette! ng U abite rose 5 Gn normal frase Sy tw stationary, prose a is folan mostly Siti apt. more _‘ fob 2 compoimels tm ih Kamba mateture — onterart yy onalens Atronaty z td Atalionamny poose . AS 3 a veut 9 swe folar comboustrel 5 | more Wy 5 while ues polar cobnfounds ‘mover higher: ‘ of tw __stationane, \foose is non folay , it > i U ] ne Positive... CE Scanned with OKEN Scanner » | DATE:_L_|__ > bs Ole yeNeY Ke, poate Ye. On this > 7 passe 2 Mon’ '- foliar Compounds move ress & > pater Combolumes omevel more Wher Kolvent 9 mmintures. aritittea> pease wth also. be mu, Y polar than iy | , h -|! tec is sof — plete ae oe fC geet : At pUte & pai bial i . ae fe: a relotivels, low cohtr Do Com monttpy yxy ares, , leterminedy purities or purtta Ame Vomounts of ® i Qa Comforenct | I @ Dusanvaadt hess : % Sle estes tom; bane) tonsey Afationane, y frase _| 0 i / Comparecf to otter Cmomatonapite —techneguers y & te _—leemath 4 separation Ved bymitede ‘ Since © Ste | ofer 03 am open eee ee ome faetovs >! burmidlitr, —& comp 8 ean affect. Zz femal outtoms Chromatogr. Woe as Oenty 2 paint atssiy ve Cechinege beat not Mpucontactive g if Column Chromatografi, . y - gt ba Qa (/ of ets pion Chroma- 3 : 7! | : 4 togro-flug Kos MOS simmcpeh pop utinn VspoNation & purification ' Ceebmedue 6 Gn | this Ceehreg ule U both otis Ug » Ligutols Cams. be’ separated & fesrefcert. ¥ 9t —_consst6 of. & Aokid stationareV ’ ees fro tat | actsobs 8 koparated the i ex on fottn aS pasuine : (aeuet it! E te A4_fi hath of "we wilh onotlot le prone | SB kaes I elolvdens Aeon song oases 9 abumine,» \ Be Positive.. Py CE Scanned with OKEN Scanner DATE:__L_|__ Flash Colum x» Chromatoqrabliry. Sn this trermgubl Vrotuent ts | sel Ea date » ate’ flow z baat { prestisne Query the fame % i Speed ok ey ou x 0 til totaly esectbant —_leombounds in & Korte | ee ae mee “efaneting he Comfporemt 4 of Jaci i iS malas os ‘bond midbentads 4 matertats ff omuttifee 4 temtmnwn crf oy j ¥ " és eeombmee due to its spel of voteniing mixtures, ce gt. is U beak for Aefariating covrBecenaih tafe 4 tom LALAES, OD imto Ds. see me : 4 iz ons. t 7 ’ sb iwtate i laetives Umavedtion bh: 4 Jo determine drun extimbies bom ’ drug formulations. j A So U x ve ty Emrfunitien % hb tsolate onetabotites feoom hévlyaceak Pd flats ( y , ON Jon emg Chromatogr n 96 isY a pefaratln | Ve teeh 0 e is usec to sehavatel bong 7 Meer , T molecules hase oo», thecy allimits, 7 a ‘ ton Cel amgers . She frvnci ble W of bh is tas Oty veuencibe — tassandy heey leet cheer tons ___ present ff Sar Bl south / : ia ” & t the tome wesent. } on the ton athoaein. ~P CE Scanned with OKEN Scanner the Charaes qroups of — : " Ag tLLOULsy KOLO)» oN the matret wilt be srrvoundbel tf ‘ 2 tw opposite Charge- ” a) en =e 4s lbw é Y 4e D tis ton) , tons can > yeuersihhkey enrehangecd Tout Changing, he ty mature d & $oUperties of, the ¢ Cater, > > Gunstrumentition ; | 5 Ion eXthAm, ge Cerna tose pig emstaremmenk - = ation imetucten Ys v 2 PemA: ie frotdes a Contimiecous > Comstamt 22 fies! af eluent, ( mobile phase ). 6) Be Positive... CE Scanned with OKEN Scanner % » pate:_/_/_ As She choice of enchonagey ctefends fon hw TP enange of | pamtecedl to! be kepanatenl: | fee lentes oI obec i sth 0 fiteet Je don % exehomger thon the samrble is 9 Tt a a nn a ee a O) fepectine 9 betper Wacetate butthy , Re) eitrabe 4ectloy J i bhoshhrate becflon'g Tk ame. a widoley sect 2 be vy. Pant e taouey > tow g ton val ORL am ae wilt a Come, ctdlon 0 eae! Column ato EE the ca bute In ew ment seep O cena > Covrespoiitiny i Aepanates toute, 4 bound Fe Nace porticles _o These, pon ticles ave 1m oes a ai Apestoos d 2 °> | See | cxctuxion Chroma Loon pl (or) | nb UU Moletutay Sieve _Chtomlathara blu, a It & a Chromatograplate ‘otha im ¢ “ vanpkeeul es...) em solution U Ove separated bey a his AE, & bn Some, Cares 44 mo 7 wietnadeen.! : U9 matty appeal te lange molernles - ov macwomotecntay I compuenes 9 "“Broteime » > emir» nucteters ais | y inctrestriat | folepmend 2 & UW. antiniotics: Y z SEC es) based on thy exclusion of conte a mpl eel es through Zo Colum cont ainim d foros headhien” oulhem am agusaens a a Sa Te et fr tee thamapereation of sampees Xe : tough eV Coltemn 5 peedure | is ‘ef » get“ fovmeation | filtvation Clromatrara phat. i) U On | tis techreque , _ etuemn contains) “ Be Positive... CE Scanned with OKEN Scanner DATE:_|_I__ porous matrix, biaiteal ‘particles 4 7 beads, Gen eralls, } Leet semn us Comfoseo} ui dextran filer ( Sbtacter) , agarose _ ( Sebrarose| 4 fold, aembamide u Sep acre | or 2toget Py rod { v In SEC, two (Lupe of). Cohumne j packuna ane morthe, vl seo} Povo dis Feces ; OS ition rons Limheo CA conte "gets ex, ertram polymers , AROXVOL EC, ete. P¢ She choc 4. pachina in Cokunnn in SEC ctefemels efor [tne molecubars. wae & Chemical brofertios of Sampte Vetat we aon, to hat separate | on, f Bioget (0 t 10) oherans for sambies & wt. bw 5000. - U [fooo Limits In & Comentional SEC Column , ehuent &s atlowed to flew wmden evawiter — The flows vate Chrotegh OD ese Colum wl Soke enie, le am émerenne” my the portiele kere. fox tom alley SChecf harticlel , the flow vate wilh 4e Lowen / She flow) vale, 5 fevthey “fqecter by the fonthene nature i” ferocity © of Veotumn beasts im SEC. Sée | worrs | by trapping Smatten _moteeily in the ores g of. he! Chromatoanaptie I Coleen, Mylecutes! tat ane hey thom the eves Ove _ umable to otitferse © in the feasts | therefore thay One eluted finse & Bemalley lente eontes q ebra te ee Wns te beacis / foes & at eluted bach. Be Positive. CE Scanned with OKEN Scanner DATE:__[__I_— mot ese | the orwhigen dace. for _ em bvactin Ete amatite 9m | gas! chvomatogy j ja stationan, pos is ott a ot i actsor bed t (' ee gas Katoh Ctacme pee oy a bitah boihing Y liqutel om |G anrl, wbtore TC eta Gos. tug ee f ” AOS - Lrareich Chromatoavafhr, , Cp kidy | hiatomaceous Carth geass fowdfey _, foweleved | teflon ete. Y lon aan ~- Kolie) Ctremmectoppa pig mventionatty, Column Y One. aches! é! tt horoies foegenlbns ey materials Is catlvatcd i Corben | vy moteeelan ALES, selica & AAamine fowler. She beguio? frese applied gemeratbtyy — bave low volatirit, | x lita, YofecombBcition Com preatirre, A g eX ., fottlotugtem @ LO stot, 2 Melt £ ene aku col puceink te A oteet# ALL Cone an J 404 Ud Chromot vqvaptsy the SMombomenst0 of tow! bamble d abel clixzolved a, a / Soluent, K | vafor eee! im exer to xefar- ate the amalte hy Lintyihuting the | sample tlw Vtwo " foasex — —_sedtionany Aoohe & mobile Hoare. | / l mobile —_fnase 5 | chemmpatt,, | tnort, gas Like He, Ne, inv 4 Lefemeting) upon "enw desineot for fsrman ee & G ae | deteety v 4etng aga! Soe VU hones ef , separation is __ bifferente in tantition [2257 votactillins oo? | Comounel bf 4" Besudy & in dorasese Be Positive. CE Scanned with OKEN Scanner the Column & get, eluted Y 5h Over @& period of J time dleperdlins naa’ opevstinal.iod Concbbtions . : In | gan - d kiguid Chromatogratiuy 4 & sotution Ques > bamble, GC mat YU Containk v ood Jo Compound $ ae I ae imeeted intl we 4 * vibe ca with bell 7 vo-povised. tbe vafourrised coe sampler are they Coryied bey imerty gas a goes thro ugh a FBLars | - : hae © peitarbe d stationald, fone & fees. tn & a thevrostatza) ovem Uo conkrok, > the Lend Ps of her Column. One of tL three thengs fe arbeglat, tabben tw @ pabtyeutar omoleete iy ie a mixture! injected | énto te Column! ro IC muy conttense’s on the stationary, — bhase. re . K. omaufl aditgobue in they leguio en id ~ Aurelie of the sta bernony U phase ee gt U 7G I yematn én the Las phase "i Combounst. Ea Aetling fore | tugtuwy thom i dw temp: of a Veet ecm with T outousbe, ee tend to / Coritm se. at the tart _ of : pe Colter l all Some mobeentes mary destotve inte tegube 2 stationan, — Paase 0 Inu move Role = wit shell —_lonove of thtin Lime _ael sorbed) ‘2 into the statlonam,! fray has Soule 2: ones with spond 0 mole time tm th _ gas et those. a QO Be Positive... 4 CE Scanned with OKEN Scanner DATE:_/_/— ah 2 ae mobile phase. She active, Component: 3 lof thy Cokeermin othe antborv ontes is 9 Cf fccrtlig a Gramutoy material orale —of — ee ae folspmszs ete 3, Je se AE Pz AEE. RL She prewsteenen ely tequid ( mobile fa) “we % z | a rd tuie of solvents + 9h of © Commpdkixion 4 Exons fey! a mafor whe aN &» thy seperation | proces" imp blemeing 2 the. tnteyactions _ Phen fesce vi bfal eee ay Aarmiple, | cannspone nts Px abtiorkemt: ase b imteractions | ane peysteol im mature dD 4 | Aectropnobic , ——etifote 1 U ctifte —-& tonto ae ee ee ee ee _ hutes out is ed _—its—ettemblon tim _ che, retention time measerrec? umdey fartterlay he 4 Conctitinns is am édemtifiing chavartenistiz, = of a aout Onaligee, aii PLE. us cesta forom —_ Craclitivn ah, _e legudal Cooma tonredbin d U ofevationg L, a: poesserres ane! | Tifa l toto ( 50 - bk 1350 bars } while U ovctinllre, O tuautol nf chromatoqrahlie, Cappicatle else on tke tex ce ie of U crabete, - O fas the mobile fhase- WV T tiyrouah Y Gee Column. | > upte! has superior __resokutmg foun ( ability yy to dstingutsh | Ww Comforlnts | [ween - separeting U anbxtinres eee | tp eae small | amount of Aarcpley esef — & —_—Colunts made e E gmatter’ —_ aclsortemt forties This makes Pe HPLC popular Chrommatnayaphic, Tchmbeue- foe i Gi! U 7 » Be Positive... CE Scanned with OKEN Scanner DATE:_L_|_— = || InsCruemenHation - 1 She 0 may Components of HPLC are ! I Dl Pamp 9 U ts hie tp | dover eg cel ¢ ono Le , dowse: } through, a& vk peribpens thow i I. / AYO / | vate ( mt [mtn ). ¢ \ ip. Injector: tt ——emttrocluces 9 tee Legusieh ‘dir bee tn the Noi stream ot ee oboe 4 Phase. j | i). || 'Cotumn: tb ts most tomtral — & tmftorCant, | Component ef HPLC. [| Detector; - te ty used ty > feted _ inolivictual moleenles thot elute from thy ») Column. CX UV - Wtsibhe Abserteamen) oteteetoy » Lroseeme __chetector , IR melon _ctotecter / Apeetino metry, ) : v) || Data buyin Va Computer» mot erty Comtrots ath the molewtes of. ype imstruement bat also takes tee) Slama feom they ofete ctor & UES te to v U determéne, thy retention time » Sample Combporext : guantetive Amat bth » f vill Degassey: Cvemoves gas U from tax ehuent bephtern Soe he omibdn ( Cause of Ompise & Cycling im the -haselimb, As ehh “fon RPE S ENS of eLurnt;: > | Ages — 2. cai ; i). Normal phase Pe: Heve the Ktatlonany Prose see} & otay ex, AELECA get! ly the ancbile | fesse Zz ren = spothy eg, hename- ii) Reveyse Fase HPLe* Here the Statronane ——— > Be Positivd. CE Scanned with OKEN Scanner 
