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AQA GCSE BIOLOGY Higher Tier Paper 1H 2026 Questions Combined with FULL MARKING SCHEME Please write clearly in block capitals. Centre number Candidate number Sumame Forename(s) Candidate signature | declare this is my own work. GCSE BIOLOGY Higher Tier Paper 1H Tuesday 13 May 2025 Afternoon Time allowed: 1 hour 45 minutes Materials ; For this paper you must have: (rel ennneirs Us ° aruler Question Mark * ascientific calculator. 1 Instructions 2 « Use black ink or black ball-point pen. 3 ¢ Pencil should only be used for drawing. 4 Fillin the boxes at the top of this page. 5 « Answer all questions in the spaces provided. ¢ If you need extra space for your answer(s), use the lined pages at the end of 6 this book. Write the question number against your answer(s). 7 ¢ Do all rough work in this book. Cross through any work you do not want 8 to be marked. ¢ Inall calculations, show clearly how you work out your answer. TOTAL Information ¢ The maximum mark for this paper is 100. « The marks for questions are shown in brackets. e You are expected to use a calculator where appropriate. « You are reminded of the need for good English and clear presentation in your answers. UuUN2584611H 01 IB/G/Jun25/G4006/E10 8461/1H Do not write outside the box The spread of measles can be reduced by vaccination. [5| Explain why vaccinating large numbers of children helps to reduce the spread of the measles pathogen in the population. [2 marks] Question 1 continues on the next page Turn over > IB/G/Jun25/8461/1H 02 Do not write . . . . . . . . outside the Figure 1 shows information about the severity of measles infection in vaccinated and box unvaccinated children. Figure 1 Key 50 {_] Vaccinated [5 Unvaccinated 40 Percentage 30 (%) of children 20 10 0 L Mild Severe Severity of measles infection [o[1].[6] There were 240 children in the survey. Calculate how many unvaccinated children had a severe measles infection. Use Figure 1. [3 marks] Number of unvaccinated children = IB/G/Jun25/8461/1H a2 [4] Name one disease that obesity is a risk factor for. Eating too much carbohydrate can cause obesity. [1 mark] Table 1 shows information about sugar intake and obesity in different countries. Table 1 Mean sugar intake Mean obesity rate Country per person per day in arbitrary units in grams A 122 18 B 80 9 c 172 25 D 150 23 E 110 15 F 144 19 G 192 32 02 IB/G/Jun25/8461/1H Do not write outside the box Do not write outside the ‘box [2] Complete Figure 2. You should: label the y-axis ¢ add the scale for the y-axis plot the data for countries C, D, E, F and G from Table 1 « draw a line of best fit. Countries A and B have been completed for you. [4 marks] Figure 2 x> 0 20 40 60 80 100 120 140 160 180 200 Mean sugar intake per person per day in grams Question 2 continues on the next page Turn over > IB/G/Jun25/8461/1H 02 Do not write . . outside the Question 2 continues on the next page box DO NOT WRITE/ON THIS PAGE ANSWER IN THE Turn over >» IB/G/Jun25/8461/1H 02 10 Sugars and starch are carbohydrates. Astudent investigated the effect of temperature on the activity of amylase. For each temperature, the student: « mixed starch and amylase together * added iodine solution after 5 minutes. The iodine solution was yellow-brown before it was added to the mixture. Table 2 shows the results. Table 2 Temperature in°C | Colour of iodine solution 10 blue-black 30 yellow-brown 60 blue-black 90 blue-black [5] explain the results at 30 °C and at 90 °C. 30°C [4 marks] 90°C 02 IB/G/Jun25/8461/1H Do not write outside the ‘box 12 Do not write outside the box Astudent investigated osmosis in pieces of potato. This is the method used. 1. Cut a piece of potato into a cube. 2. Record the mass of the cube. 3. Place the cube into a beaker containing 0.4 mol/dm® salt solution. 4. After some time, remove the cube and record the mass. 5. Calculate the change in the mass of the cube. 6. Repeat steps 1 to 5 using two more different concentrations of salt solution. [4] Evaluate the student’s method. You should include how the student could improve the validity of their results. [6 marks] 02 IB/G/Jun25/8461/1H 13 Do not write outside the box [2] Why would a potato cube placed into distilled water increase in mass? [1 mark] Tick (“) one box. The solution in the potato cells was less concentrated than the distilled water. The solution in the potato cells was more concentrated than the distilled water. The volume of water in the potato cells was greater than the volume of distilled water. The volume of water in the potato cells was less than the volume of distilled water. [3] Active transport is another process used by potato cells. Give one way active transport is different from osmosis. [1 mark] Turn over for the next question Turn over >» 02 IB/G/Jun25/8461/1H 15 Do not write outside the box Astudent investigated the effect of temperature on the rate of photosynthesis in pondweed. Figure 4 shows how the student set up the apparatus. Figure 4 =—1— Bubble + — Pondweed -+— Water Y This is the method used. = Prepare five tubes of pondweed as shown in Figure 4. 2. Place one tube into each of five water baths set at different temperatures. 3. Wait two minutes. 4. Measure the time taken for each piece of pondweed to release 10 bubbles. 4] What is the independent variable in the investigation? [1 mark] Question 4 continues on the next page Turn over > 02 IB/G/Jun25/8461/1H Table 4 shows the results. 16 Table 4 Temperature Time taken for pondweed to in °C release 10 bubbles in seconds 10 84 20 43 30 27 40 25 50 33 [2] Calculate the rate of photosynthesis at 20 °C. Give your answer to 3 significant figures. Give the unit. [4 marks] Rate (3 significant figures) = Unit = 02 IB/G/Jun25/8461/1H Do not write outside the box 18 A gardener noticed that some plants were not growing as well as usual. [6] Describe two ways the gardener could identify a plant disease. [2 marks] 1 2 One of the plants had purple spots on its leaves and the leaves fell off. What disease is the plant most likely to have? [1 mark] {8| A different plant had yellow leaves, but no spots. Explain why plants may develop yellow leaves. [2 marks] Do not write outside the ‘box 02 IB/G/Jun25/8461/1H 19 Do not write outside the box This question is about the human circulatory system. [4] Blood plasma transports blood cells around the body. Give one other function of blood plasma. Do not refer to oxygen in your answer. [1 mark] Question 5 continues on the next page Turn over > 02 IB/G/Jun25/8461/1H 
