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it is from professor schul class lab for adropsosis
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Bio 1500 – Arabidopsis Lab Module Arabidopsis Lab 3 worksheet (15 points) Student name: Yosan Ghebremichael Lab group name/number: 2F Date: 4/15/ NOTE: Please ensure you fill in the worksheet during the lab and upload a copy of it to Canvas by the end of the lab day. Checking your background knowledge Q1. In your groups discuss some of the distinct differences you expect to see in plants grown in dark vs. light conditions. The response in the picture below is related to PhyB gene and Red Light. How do you expect the mutants for this gene to grow in dark, red, and blue light? (1. points) Dark: long, thin, pale seedlings (etiolated growth) Red light: still look like dark-grown plants because they cannot detect red light properly Blue light: grow like wild type because blue light uses a different system Q2. Within your groups discuss some of the distinct differences you expect to see in plants grown in dark vs. blue light from side conditions. The response in the picture below is related to Phot gene and Blue light.
How do you expect the mutants for this gene to grow in dark, red, and blue light? (1. points) Dark: long, pale seedlings (normal dark growth) Red light: grow like wild type (normal growth) Blue light: look like dark-grown seedlings because they cannot respond to blue light Q3. Why do you think we needed to keep the seeds in the dark, along with our light treatments? ( 1 point) For a control. Seeds were kept in the dark as a control condition to compare against light treatments. This ensures that any observed differences in growth are due to light exposure instead of other differences. Q4. All plates were kept in the same room and at room temperature. Why do you think we kept both sets of seeds (dark and light treatment), at the same room temperature? ( 1 point) So we don’t have any confounding variables. Both treatments were kept at the same room temp to ensure that temperature was not a confounding variable and the differences in plant growth was due to light conditions only.
What is the value of plotting data (using histogram here), and calculating the mean and standard deviation? ( 1 point) Histograms help visualize the distribution and variability of seedling growth data. The mean shows the average plant length for each group, while the standard deviation shows how much variation exists within the data. Together, these values help determine whether differences between treatments are consistent and meaningful. And what type of graph might be more suitable for your data? Why. ( 1 point) Bar graph with error bars is the most suitable because it clearly compares average plant height across light conditions and genotypes while also sowing variability whithing each group. Save a copy of your group graph/s here. (1 point)
Individually, write a few sentences summarizing the overall steps of the study conducted today. You don't need to document every single step but try to summarize the key activities done in your own words, so someone else can have a good understanding of what was done today. ( 2 points) We took pictures of our grid agar plates from last class with a ruler beside it. We then, pulled up the image on ImageJ using the Fiji software to measure the seedlings length because we had phyB deficient mutant. We set our scale to one cm using the picture and measured everything sing plant growth. We then charted it on the excel Time to reflect on today’s lab: In today’s lab, which tasks/activities did you focus on to help your team? ( 1 points) o Helped with collecting/measuring/recording data o Helped with taking pictures and notes o Helped with analyzing data and creating graphs o Uploading the data file on Canvas on behalf of the group by this Friday Add if you have helped in any other areas too: I Collected the data (height of the plant) for the WT and Mutant for the Red light plants. I also put my information in the excel sheet and calculated the Average, Range, Standard deviation for those that I took data from. Muddiest points: In this lab, What was the most interesting part? (0.5 point)