CHE260 Biochemistry NCC
Rakhi Agarwal, Ph.D
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Purifying Proteins by Column Chromatography!
Post Lab Assignment
1. What is the basic principle of the Chromatography technique in general in the separation of a
single sample from the mixture?
2. Name at least 3 different types of Chromatography techniques and the strategy used in the
separation of a sample (s)?
3. What are Wash buffer in column Chromatography and its role?
4. What are the Elution buffer and its role?!
