BIOD 171 Lab 9 - Notebook Unknown pathogen analysis
AH09 Unknown Pathogen Analysis
Objective: To identify an unknown pathogen(s) based on the given
assays developed during this course.
Notes:
• Two samples from the hospital was brought to your lab for
diagnosis. After culturing the samples on LB agar plates another co-
worker began the analysis. However, due to an emergency your co-
worker has left the remaining work to you. Before leaving your co-
worker informs you the 2 liquid culture samples were accidentally
combined before the Gram stain was started. However, the 2cultures
remain separated on LB plates for future tests.
o GOAL: To correctly identify the 2 pathogens.
Results:
• Gram (+) coccus clusters
• Gram (-) bacillus rods
• MacConkey plate
o Culture A – no growth
o Culture B – lactose fermentation is present
▪ Zone 1 – growth is apparent
▪ Zone 2- colony isolates begin to form.
▪ Zone 3 – single colony isolates.
• BAP-
o Culture A- beta-hemolysis
o Culture B- no beta-hemolysis
• EMB-
o Culture A- No growth
o Culture B- colonies with green metallic sheen
• Catalase Test-
o Culture A- positive
