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A series of questions and answers related to a microbiology lab practical exam. It covers topics such as gram staining, acid-fast staining, endospore staining, bacterial motility, and the use of microscopes. The questions are designed to test students' knowledge of bacterial identification, staining techniques, and microscopy. It provides a concise review of key concepts and procedures commonly encountered in a microbiology laboratory setting. This material is useful for students preparing for lab exams or seeking to reinforce their understanding of basic microbiology techniques. It serves as a practical study guide, offering direct questions and answers that facilitate quick learning and retention of essential information.
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Bacillus cereus - correct answer ✔✔Give an example of a Gram Positive bacteria Escherichia coli - correct answer ✔✔Give an example of a Gram Negative bacteria Mycobacterium smegmatis - correct answer ✔✔Give an example of a Acid-fast Positive bacteria Bacillus cereus - correct answer ✔✔Give an example of a Acid-fast negative bacteria Bacillus cereus - correct answer ✔✔Give an example of a endospore positive bacteria Mycobacterium smegmatis - correct answer ✔✔Give an example of a endospore negative bacteria One cell - correct answer ✔✔What is the minimum number of bacteria that it takes to start a colony? Prevent condensation - correct answer ✔✔Why are agar plate incubated upside down? Create the same environment as human host - correct answer ✔✔Why are agar plates incubated at 37 degrees Celsius? 4x - correct answer ✔✔What is the magnification of the scanning scope? 10x - correct answer ✔✔What is the magnification of the low-power scope? 40x - correct answer ✔✔What is the magnification of the high-power scope?
100x - correct answer ✔✔What is the magnification of the oil immersion scope? 10x - correct answer ✔✔What is the magnification of the ocular lens? Objective lens x ocular lens - correct answer ✔✔How to calculate TOTAL magnification? Crystal Violet - correct answer ✔✔What is the primary stain in gram staining? 95% Ethanol - correct answer ✔✔What is the decolorizer in gram staining? Safranin - correct answer ✔✔What is the counterstain in gram staining? Whether the bacteria has a thick or thin peptidoglycan layer - correct answer ✔✔What is the Gram stain designed to differentiate in bacterial species? Purple - correct answer ✔✔What color do gram positive bacteria stain? Pink - correct answer ✔✔What color do gram negative bacteria stain? Carbolfuchsin - correct answer ✔✔What is the primary stain in acid-fast staining? Acid alcohol - correct answer ✔✔What is the decolorizer in acid-fast staining? Methylene blue - correct answer ✔✔What is the counterstain in acid-fast stain? Pink - correct answer ✔✔What color do acid-fast positive bacteria stain? Blue - correct answer ✔✔What color do acid-fast negative bacteria stain?