Biotechnology principles and processes (ncert based), Lecture notes of Biology

Biology 2024 Neet Gracy Akansha aggarwal

Typology: Lecture notes

2023/2024

Available from 05/24/2025

gracy-dudeja
gracy-dudeja 🇮🇳

2 documents

1 / 11

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
pf3
pf4
pf5
pf8
pf9
pfa

Partial preview of the text

Download Biotechnology principles and processes (ncert based) and more Lecture notes Biology in PDF only on Docsity!

ya il Paincple a and precy EFB- Fed Riot gin a lifinition ot ea Be beaiiithe vi ome mad th. Ante y biotechnoloyy Th -clifinttion Gfiven iera> it Th lain d Natrol deiere and onpanisns, £UlL , port Huxe of ampleeelar .amalogius for oprccduacls nd swith. Hel fi — Techngue to alter ie tenia sas, eigintons a and RWA) , the (eharge) chemistry ¢ inward Fee rn ie tho engantiont and i + organism Ate phenotype of the Aov le Aioprocrs ompincentag|- stesttle Conditforr- [microbiaS content Og qn Chemical engl fincextng to enable th Lyspwth of) omy Wo sated Dior foukan te Pn Lange Quantthe . ] ue fLfottes 5 yacchns Frocueb pews rom Bfotechnolegtcel eae pre / ] =~ = NCERT Heacling = 72), Cohn sty ane| Hocbert Boy © dened gene] 4 ~ si une Fide — ; [recombinant pn] ® mi oe yeatore - Iv “ on x NDNA [A +8] a — plaamed af) extra chromosom sd ane 4 ce eailp ds DNA: ¥ “ 7 auronaay y|4 Retard 4. ‘e) | ? Fini bio esigtant Wei 7 aha p + Oni dead on I Luke ae aah Srey of C ssi ble due 7 (colt) [No Aniifot< J we ttaodton eit sind! fue By @ Resistance ) -] “if 1 & | pr Progen F.colr” Paar re |» | OU J ¢ aut, ToR) @ i} = i wi “a ) 9 fintibrotrs. MA ck P HOT seuiatant | {ix Tose oH bended ANA SE eee d “Tools of Recombinant ANA “Techmolog 7 +. (Ezy ES: — fon isolation 9 purified ENA. a) Aigeet cell wall - (Plant » Algal toll) —> Cellulose Peatinase . ° all Gall ==) chr finase ° Raetertal cell Aipory me b) Digut Plasma pmb. Nucla, momb — Ddvgees RNa = aNAoxe wo. | cl d) Aigest Poupteins > Protease | pa + RNA * puotein = chromatin | Glace alled \} erfilled ethanol U DnA pretpi out a tine we Suspension” J dioladed psig’ QNA jeumouo| 7 $y pooOLIN Gy y festxictfon bl deyuclease Rest ei chon enyyys I, . Molecular leaner elle: 22 pA inte ics | : Moe Nucdease\— duet Nuclue Acco behak 3,5 ifort Bond - eae bee eS ee exonuClease AV siy.o: peetaG” © b A ad Fe Luk in inner perieale peal: i) - pout elCncks mit baer metmeedat (Tt. sinond ov Palindrorue | Lut 2 Gh Pine Reatidction AT on. hath # Hind D (fo freioune ape tant Tale Pe [arcanc] [QTC GAG») CAG CTS 3! eae hag 5) AP ion RE Aur OMA of Leth L4xamds y sigh tty al Aitts ) 8 aoe wn liges form phas be dexter wd akith was Balk 4 Restnichon athe K Veet BNA and downer pwr Shows ut sith Somt RE, (qn, DNA figos Hh, ee CO | Pogponte ate for BNA “Replication — CIEL ELecteo . By y Cems 970 All Oidiloiesys 2 aah On ang Holafion “9 se Gel tleetrophonut of ORR {hay =, A - SSO SMGTE LES ( 0) saechaxtdle) > petty —> Natu rt, erica = ohtawud foro Garcia 4 om fe : Sen iee x Sy he Arca (Aas my (eal _ age calbt it fea weal | y —-_ ~ Seivet 2 = a (ee, - 2, c - — tnd Gon get finvcle a dane (1) Sura gested DNA | Wl a, af Smolin yor } Oe fede Th ampves - 7s + pee Ae on ee ize esa ey nS menage Ff DUD fragment visiualixed a Metniadtori Bromide 5 fowal by UV megs. than BRIGHT ORANGE BAND Yisualized. Separated hands Cudtfnom ogres 4 anid Saga Feom od fs, 2a Te cold or > ¢LUTION ai by Gel cae wala pene: pwr Lan also check NO} rages a] Aopen Netty 7 AE [re 2) Veetox | nbNA iS > Won- ‘Tr (Oo ) x on Reconbinaad 3) Veron |» DNA oy 0 a paste, \ Select Beleatable vmaxpers tutpe fn tdeubfy waned Le Hrami formed tom tne raf) = ce! mon) L e ed Ayrostr SelecHvely Te ) i _& hi) dnactivat ev: —> cambuu one Por . ‘eC veetor [Plasmid ] . + HOST into . N Lame | Tota Se, alive alive | ative. Ty | die | div | te pie die jobs | dee pelabine |e | dee oa june oy J ie = ey pA as vf * x fnpcltin» ‘Terocyetin Chloramphenicol, Kanasngpne ; (HOST = E-coli ). B bee tps — 3] ecg ne S Host tramaporomed 3 Non Recombinant - (ony veorr into host) Mot HRBA: feo: Z Active. es dubstrate eee L pure color S303 transformed cu te fn yorgte colour colowls vest + aes ae at oh Bolivers pls Rodda yeotoles rmutheds. 0) Micro fn ection > Recombfrort DNA " epee ( ae inte nici li Fy) c d (2 Afolisti or Gyem Gun — cSultable for paw; cells High elo . nuit bombatoud with Cie ida old or tungsten coated apes ‘ a “ ) chen Mettod,. @ polyottyunr Glyuol > (es () clewlat 87 boss tad, - ‘ lificati ] + SE Saath OF Gg — P of DNA | Grene ¢ fntereat tn vin : =— j DNA tobe amplified e (outside Body). i jas ncaa — — 3) oe TT 2 as, 40-45 —s {| phar on Bonds Bruak » Both strands Lp | pias ICs amy 5 AD fiadition °) 2 primers Te es. He neo fo = a Sl ; —a Oo aeey G ee Be TA —-+ +, yucluotidsa[ nertuatel - b Rolywe LT eneyee |. Frag, f a ss as a ; + es fy CA Say + | 3a Ale : | UG a eee eee, pple do cycle ai" pilion coptas } + Ternpexatuee Be renai | Rounol yee +f Tay (ee i eae ar an enzyme rom Thermal aguafice. afer — Theumostable [fers PEt Noc),