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Cellular Respiration 1
Cellular Respiration
How is energy transferred and transformed in living systems?
Why?
Living organisms display the property of metabolism , which is a general term to describe the processes carried out to acquire and use energy. We know that people need to eat, and in our foods are various kinds of nutrients that our cells use. One large group of nutrients in our foods is carbohydrates, which supply our cells with glucose (C 6 H 12 O 6 ). So the question is: How does the food we chew and swallow fuel our cells?
Model 1 – Glycolysis
! = pyruvic acid (3 C) = glucose (6 C) = mitochondrion
= nucleus
NAD
NAD NADH
NADH
ATP
ATP
ADP
ADP
Cell membrane
- Refer to Model 1.
a. What is represented by the hexagon?
b. How many carbon atoms (C) are in one molecule of glucose?
- Refer to Model 1.
a. What is represented by the triangles?
b. How many carbon atoms (C) are in one molecule of pyruvic acid?
- In the process of glycolysis, what happens to glucose after it crosses the cell membrane into the cytoplasm of the cell?
2 POGIL™^ Activities for High School Biology
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Glycolysis occurs in the cytoplasm of cells and does not require the presence of oxygen. Therefore, the process is anaerobic. It is the fi rst step used by cells to extract energy from glucose in the form of ATP. ATP can be directly used by cells.
- Thinking about the number of carbon atoms in glucose and in pyruvic acid, explain why there is one molecule of glucose on the left side of the arrow and two molecules of pyruvic acid on the right side of the arrow.
- How many ATP molecules are produced during glycolysis?
- Hydrogen-carrying molecules are also produced during glycolysis. What is the symbol of these hydrogen-carrying molecules?
- Does glycolysis occur inside or outside the mitochondria?
Model 2 – Krebs Cycle
Mitochondrial matrix Innermitochondrial membrane
Outer mitochondrial membrane = carbon dioxide (1 C)
NAD+
NAD+^ NAD+
NAD+
NADH
NADH NADH
NADH
FADH (^2)
ADP ATP
FAD
= pyruvic acid (3 C)
- According to Model 2, what happens to pyruvic acid during the Krebs cycle?
- According to Model 2, where does the change identifi ed in the previous question occur?
4 POGIL™^ Activities for High School Biology
Model 3 – The Electron Transport Chain
e–
e–
H+
Inner mitochondrial membrane
Outer mitochondrial membrane (^) H+ (^) H+
H 2 O
H+
ADP ATP
e–^ = electron = oxygen
NADH
Mitochondrial matrix
NAD +
FADH 2 FAD
- What cell structure is the site for the electron transport chain?
- Label the carrier proteins in Model 3.
- What substance do the carrier proteins transport across the inner mitochondrial membrane?
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NADH and FADH 2 molecules release hydrogen ions that are transported across the inner mitochondrial membrane with the help of electrons. The result of these multiple processes is the production of large amounts of ATP.
- What high energy molecules are formed by the electron transport chain?
- Refer to Model 3.
a. What atom accepts the hydrogen ion at the end of the electron transport chain?
b. What molecule is formed as a product of that acceptance?
- Formulate an explanation for why the events of the electron transport chain constitute an aerobic process rather than an anaerobic process (like glycolysis).
Cellular Respiration 5
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Remember that glycolysis produces two pyruvic acid molecules per glucose molecule along with two of the hydrogen-carrying NADH molecules. Remember also that the Krebs cycle produces NADH as well as another hydrogen carrier called FADH 2. It is important to know that during the electron transport chain, when each NADH gives up electrons and hydrogen ions, there is enough of a potential energy change to make three ATP molecules. When each FADH 2 gives up electrons and hydrogen ions, there is enough of a potential energy change to make two ATP molecules.
- Fill in the chart below to calculate the total amount of ATP produced from the breakdown of each glucose molecule during the three steps of cellular respiration.
Number of ATP produced from one glucose molecule
Number of H-carriers produced from one glucose molecule
NADH FADH 2
Glycolysis
Krebs Cycle
Electron Transport Chain _______________^ x 3 x 2
Total ATP Produced
Grand Total ATP produced (add all 3 columns above)
- Look at the equation for cellular respiration and write in which stage of the process each mol- ecule is either used or produced.
C 6 H 12 O 6 + 6O 2 → 6CO 2 + 6H 2 O + 38 ATP
Used in Used in Produced in Produced in Produced in
- Compare the ATP available to cells when oxygen is present versus when it is absent. How might this help explain why brain and heart functions are so quickly affected when a person cannot breathe?
