Clinical Laboratory Diagnostics, Exams of Advanced Education

A wide range of topics related to clinical laboratory diagnostics, including microbiology, hematology, biochemistry, and immunology. It presents various test results, interpretations, and follow-up actions for different medical conditions and scenarios. The document delves into the identification of microorganisms, analysis of blood and urine samples, and the interpretation of diagnostic tests. It covers topics such as antibiotic resistance, hyperlipidemia, coagulation disorders, and viral infections. The information provided can be useful for medical professionals, students, and researchers in the field of clinical laboratory science to enhance their understanding of diagnostic procedures and clinical decision-making.

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2023/2024

Available from 08/15/2024

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MLS(ASCP/ASCPI) Exam With 100%
Correct Answers 2023
unconjugated bilirubinemia:
unconjugated bilirubin=positive; urine bilirubin=positive; urine urobilinogen=positive
unconjugated bilirubin=negative; urine bilirubin=positive; urine urobilinogen=negative
unconjugated bilirubin=positive; urine bilirubin=negative; urine urobilinogen=positive
unconjugated bilirubin=negative; urine bilirubin=negative; urine urobilinogen=positive -
Correct Answer-unconjugated bilirubin=positive; urine bilirubin=negative; urine
urobilinogen=positive
what is measured in the urease method determination of BUM?
uricase
Urea
NAD
Nitrogen - Correct Answer-NAD
what is the specimen of choice for FK506 (Tacrolimus), Cyclosporine, and Sirolimus?
gray top
heparin sodium
lavender top (EDTA)
red top - Correct Answer-lavender top (EDTA)
IN chronic active hepatitis, high titers of which of the following antibodies are seen?
Anti-smooth muscle
Antimitochondrial
anti-DNA
Anti-parietal cell - Correct Answer-Anti-smooth muscle
Which of the following is used to compare two sets of mean?
Chi-square
CV
Standard Deviation
Paired T-test - Correct Answer-Paired T-test
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MLS(ASCP/ASCPI) Exam With 100%

Correct Answers 2023

unconjugated bilirubinemia: unconjugated bilirubin=positive; urine bilirubin=positive; urine urobilinogen=positive unconjugated bilirubin=negative; urine bilirubin=positive; urine urobilinogen=negative unconjugated bilirubin=positive; urine bilirubin=negative; urine urobilinogen=positive unconjugated bilirubin=negative; urine bilirubin=negative; urine urobilinogen=positive - Correct Answer-unconjugated bilirubin=positive; urine bilirubin=negative; urine urobilinogen=positive what is measured in the urease method determination of BUM? uricase Urea NAD Nitrogen - Correct Answer-NAD what is the specimen of choice for FK506 (Tacrolimus), Cyclosporine, and Sirolimus? gray top heparin sodium lavender top (EDTA) red top - Correct Answer-lavender top (EDTA) IN chronic active hepatitis, high titers of which of the following antibodies are seen? Anti-smooth muscle Antimitochondrial anti-DNA Anti-parietal cell - Correct Answer-Anti-smooth muscle Which of the following is used to compare two sets of mean? Chi-square CV Standard Deviation Paired T-test - Correct Answer-Paired T-test

Duckert's Test 5M Urea Solubility Test is used of: Factor II deficiency Factor XIII deficiency Factor VIII deficiency Factor XII deficiency - Correct Answer-Factor XIII deficiency What is the treatment for warfarin toxicity? vitamin K cryoprecipitate Factor IX concentrate Factor VIII concentrate - Correct Answer-vitamin K Excessively pink stain causes: prolonged washing time mounting coverslips before they are dry too high acidity of the stain AOTA - Correct Answer-AOTA type of anemia of hookworm infection: normocytic, normochromic microcytic, hypochromic macrocytic, normochromic normocytic, hypochromic - Correct Answer-microcytic, hypochromic In emphysema, the absence of alpha-1 globulin peak is due the deficiency of: A1-Antitrypsin (AAT) Macroglobulin Hemopexin Transferrin - Correct Answer-A1-Antitrypsin (AAT) normal value for mean corpuscular volume (CV): 50-80 fl 100-200 fl 80-100 fl 60-90 fl - Correct Answer-80-100 fl Drug toxicity screening of a 6 year old girl has increased presence of ACETAMINOPHEN. What test should be done next? ALT and AST

crystals protein immunoglobulin AOTA - Correct Answer-crystals monosodium urate crystals are seen in: gouty fluids leukocytes rhomboids chondrocalcinosis - Correct Answer-gouty fluids Urate crystals are generally needle-shaped and may be found within: gouty fluids leukocytes rhomboids chondrocalcinosis - Correct Answer-leukocytes calcium pyrophosphate crystals are present in: gouty fluids leukocytes rhomboids chondrocalcinosis - Correct Answer-chondrocalcinosis calcium pyrophosphate dehydrate crystals usually appear as: gouty fluids leukocytes rhomboids chondrocalcinosis - Correct Answer-rhomboids What to do? mother: type "O", Rh negative, no antibody to D Ag Child: Type "O", Rh positive transplacental transfusion RhIg administration Do nothing, report results DAT - Correct Answer-RhIg administration sodium fluoride as anticoagulant: binds antithrombin prevents glycolysis

no effect at all NOTA - Correct Answer-prevents glycolysis Eosinophils in LAP (leukocyte alkaline phosphatase) score: not counted double the value constant value NOTA - Correct Answer-not counted the formation of germ tubes presumptively identifies: candida tropicalis candida albicans candida glabrata candida parapsilosis - Correct Answer-candida albicans "Whooping cough bacillus": Bordetella bronchiseptica Bordetella pertussis Haemophilus aegyptius NOTA - Correct Answer-Bordetella pertussis prolonged T and APTT with depleted fibrinogen levels and platelet can be associated with: Acute DIC Von Willebrand Disease Hemophilia A Glanzmann's Thrombasthenia - Correct Answer-Acute DIC In what blood group type expression of antigens is weakened or in some cases negative during pregnancy? P Lewis i/I Kell - Correct Answer-Lewis Polycythemia vera mutation? ADAMS JAK Rpp Bcl/BCR - Correct Answer-JAK

the drug procainamide is prescribed to treat cardiac arrhythmia. What biologically active liver metabolite of procainamide is often measured simultaneously? N-acetylprocainamide (NAPA) primidone phenobarbital benzoylecgonine - Correct Answer-N-acetylprocainamide (NAPA) These inclusions are found within red blood cells composed of denatured hemoglobin. It can also be seen in cases of moth ball (naphthalene) ingestion. heinz bodies basophilic stippling howell-Jolly bodies Auer rods - Correct Answer-heinz bodies Portwine urine color: antibiotics intake cola intake bilirubinemia porphyria - Correct Answer-porphyria which of the following causes antibody against TSH? SLE Grave's Disease Hashimoto's Thyroiditis Rheumatoid Arthritis - Correct Answer-Grave's Disease which of the following is associated with Alder-Reilly inclusions? two-lobed neutrophils mucopolysaccharidosis membrane defect of lysosomes Dohle bodies and giant platelets - Correct Answer-mucopolysaccharidosis thrombocytosis: 1000 x 10^9/L, functionally abnormal platelets: essential thrombocythemia polycythemia vera absolute thrombocythemia NOTA - Correct Answer-essential thrombocythemia A patient with walking pneumonia is being treated with penicillin. State one possible condition why the patient is not recovering:

the organism produces beta lactamase the organism has no cell wall the organism has capsule the organism produces an enzyme that neutralizes the antibiotic - Correct Answer-the organism has no cell wall what is the coefficient of variation if the mean is 90 and the standard deviation is 5.48?

30 - Correct Answer-6. which of the following is the agent of hand, foot, and mouth disease? herpes coronavirus coxsackie A retrovirus - Correct Answer-coxsackie A Identify the organism: Bile esculin positive; 6.5 NaCl: positive, PYR negative; LAP negative group D strep Group B strep Leuconostoc Enterococcus - Correct Answer-Leuconostoc what does the pH measurement needs? pH with known buffer at constant temperature pH with unknown buffer at constant temperature pH with known buffer and constant HCO3 concentration NOTA - Correct Answer-pH with known buffer at constant temperature FBS: 120 mg/dl, OGTT: 140 mg/dl non-diabetic diabetes mellitus

Pasteurella multocida actinomyces israelii bartonella henselae leptospira interrogans - Correct Answer-Pasteurella multocida shelf life for CPDA- 35 days 12 days 21 days 1 year - Correct Answer-35 days reference ranges are performed in a new methodology to: test instrument precision test instrument accuracy assess control ranges AOTA - Correct Answer-test instrument precision the principle of the reagent strip test for urine protein depends on: copper reduction protein error of indicators enzyme reaction toluidine reaction - Correct Answer-protein error of indicators the growth of which of the following fungi will be enhanced by olive oil? Malassezia furfur T mentagrophytes T. rubrum NOTA - Correct Answer-Malassezia furfur supervisor acts according to the mutual consent and discussion or in other words, he consults subordinates in the process of decision making. This is also known as participative of consultative supervision. democratic autocratic laissez-fair AOTA - Correct Answer-democratic the supervisor wields absolute power and wants complete obedience from his subordinates. He wants everything to be done strictly according to his instructions and never likes any intervention from his subordinates.

democratic autocratic laissez-fair AOTA - Correct Answer-autocratic this is also known as independent supervision. Under this type of supervision, maximum freedom is allowed to the subordinates. The supervisor never interferes in the work of the subordinates. democratic autocratic laissez-fair AOTA - Correct Answer-laissez-fair sterilization of autoclave: 121 C at 20 lbs/psi for 30 minutes 121 C at 15 lbs/psi for 15 minutes 85 C for 15 lbs/psi for 30 minutes 100 C for 20 lbs/psi 50 minutes - Correct Answer-121 C at 15 lbs/psi for 15 minutes Auer rods are most likely present in which of the following: Acute myelocytic leukemia erythroleukemia chronic myelocytic leukemia burn patient - Correct Answer-Acute myelocytic leukemia Schales and schales method is a mercurimetric titration used in the analysis of: chloride calcium phosphorous chromium - Correct Answer-chloride IN purified protein derivative (PPD) skin test for tuberculosis, T cell mediates what type of hypersensitivity? type I type IV type II type III - Correct Answer-type IV Alpha-1 globulin flat curve in a protein electrophoresis indicates?

A relative isolate is being spread from patient to patient - Correct Answer-There has been a break in proper preparation before drawing blood for culture Phenomenon in which large quantities of toxins are released as the bacteria dies during treatment: swarming phenomenon tonegawa phenomenon Jarisch-Herxheimer phenomenon Ellie-Metchnikoff phenomenon - Correct Answer-Jarisch-Herxheimer phenomenon A yellow colony from a wound culture tested catalase-positive and coagulase negative. The organism stained as gram-positive cocci in clusters. Which of the following tests would differentiate between a coagulase-negative Staphylococcus and Micrococcus? novobiocin susceptibility Furazolidone (100 ug/disk) susceptibility bile esculin leucine aminopeptidase production - Correct Answer-Furazolidone (100 ug/disk) susceptibility Most common error in Polymerase chain reaction (PCR) is nucleic acid contamination. true false - Correct Answer-true when using winged blood collection device (butterfly) for coagulation study: use non-additive top and transfer to blue top a waste tube should be drawn first do nothing, just collect sample right away NOTA - Correct Answer-a waste tube should be drawn first principle of reagent strip of pH: double indicator system change in dissociation constant (pKa) sodium nitroprusside reaction NOTA - Correct Answer-double indicator system Which of the following test is used to identify Hairy Cell Leukemia (HCL)? myeloperoxidase Tartrate-resistant acid phosphatase Tartrate-resistant alkaline phosphatase sudan black B - Correct Answer-Tartrate-resistant acid phosphatase

What is the biosafety level and biologic safety cabinet used for Poxvirus? biosafety level III; safety cabinet II biosafety level I; safety cabinet III biosafety level II; safety cabinet II NOTA - Correct Answer-biosafety level II; safety cabinet II tap-water bacillus: M. gordonae M. tuberculosis M. avium M. smegmatis - Correct Answer-M. gordonae An antiepileptic (or anticonvulsant) used to control seizure disorders is: acetaminophen lithium phenytoin digoxin - Correct Answer-phenytoin what is the formula for sensitivity? TP/(TP+FN)x TP/(TP+FP)x (TP+FN)/TPx (TP+FP)/TNx100 - Correct Answer-TP/(TP+FN)x which of the following would show dosage? anti-e anti-M anti-K anti-Le(a) - Correct Answer-anti-M causative agent of eye conjunctivitis: C. trachomatis H. ducreyi L. monocytogenes T. pallidum - Correct Answer-C. trachomatis what is correct polymerase chain reaction (PCR) sequence? annealing, extension, denaturation

17-ketosteroids are produced in the: adrenal glands hypothalamus thyroid glands pituitary glands - Correct Answer-adrenal glands elevated level of aminolevulinic acid in urine is due to the presence of? magnesium copper lead zinc - Correct Answer-lead gram positive bacilli; non-motile; non-hemolytic; catalase positive; spore forming: B. anthracis B. cereus N. meningitidis N. lactamica - Correct Answer-B. anthracis hemolysis pattern of Streptococcus pneumoniae: alpha hemolytic beta hemolytic gamma hemolytic AOTA - Correct Answer-alpha hemolytic which of the following is the product of purine metabolism? ammonia creatinine uric acid bilirubin - Correct Answer-uric acid which of the following patients is most at risk for hyperosmolar non-ketotic coma? 70 yr-old, type 2 diabetic 30 yr-old type 2 diabetic 40 yr-old hypertensive patient - Correct Answer-70 yr-old, type 2 diabetic cause of false negative ABO typing rouleaux positive DAT 37 C

LISS - Correct Answer-positive DAT blood agglutination is noted. What can the MLS do to differentiate it from rouleaux? prewarm dilute with 22% albumin dilute with normal saline - Correct Answer-dilute with normal saline what is the significance of eosinophils in urine? kidney tumors acute interstitial nephritis parasitic infection UTI - Correct Answer-acute interstitial nephritis biochemical reaction: bile esculin positive 6.5% NaCl negative Streptococcus bovis Streptococcus pneumoniae Enterococcus faecalis Enterococcus faecium - Correct Answer-Streptococcus bovis Which assay, using 24-hour urine, is considered the BEST single screening test for PHEOCHROMOCYTOMA? homovallic acid (HVA) metanephrines Vanillymandelic acid (VMA) catecholamines - Correct Answer-metanephrines pH used for Malarial parasite staining: pH 6. pH 5. pH 7.2 - Correct Answer-pH 7. SSA (+); protein reagent strip (-): presence of protein other than albumin false positive result false negative result specimen too old - Correct Answer-presence of protein other than albumin interpret and determine what is the cause:

Which of the following is best to visualize oval fat bodies and crystals? interference contrast phase contrast polarizing bright-field - Correct Answer-polarizing the optimum storage temperature for platelets is: -20 degrees celsius -12 degrees celsius 22 degrees celsius 2 degrees celsius - Correct Answer-22 degrees celsius which of the following should be tested in cases of Antacid overdose? pH potassium ammonia chloride - Correct Answer-pH ionized calcium was left standing for a while. What change would happen to the sample? Change in pH evaporation A and B NOTA - Correct Answer-Change in pH presence of Philadelphia chromosome (t9;22); LAP 0 acute lymphocytic leukemia (ALL) Chronic Myelogenous Leukemia (CML) Acute myelogenous leukemia - Correct Answer-Chronic Myelogenous Leukemia (CML) increased levels of TdT (Terminal deoxynucleotidyl transferase) activity are indicative of: acute lymphocytic leukemia acute myelocytic leukemia Burkitt lymphoma Eosinophilia - Correct Answer-acute lymphocytic leukemia High-level Aminoglycoside Resistance

micrococcus staphylococcus enterococci - Correct Answer-enterococci which blood group system is associated with resistance to P. vivax malaria? P Duffy Kell Kidd - Correct Answer-Duffy checking and reporting of patient value difference from previous analysis is term as: delta check quality check sieving trend - Correct Answer-delta check which of the following pigments will deposit on urate and uric acid crystals to form a precipitate described as "brick dust"? urobilin bilirubin uroerythrin urochrome - Correct Answer-uroerythrin 10% KOH was place to a vaginal discharge for microscopic examination. After addition, fishy odor comes out: trichomonas Gardnerella vaginalis Staphylococcus saprophyticus - Correct Answer-Gardnerella vaginalis the binding strength of an antibody for an antigen is referred to as: avidity specificity affinity titer - Correct Answer-avidity most potent activator enzyme: magnesium sodium potassium - Correct Answer-magnesium