Complete Plasmid DNA Sequencing
CD Genomics provides complete plasmid DNA sequencing service, and
our improved bioinformatics pipelines are available to perform de
novo assembly with no reference required.
The Introduction of Complete Plasmid DNA Sequencing
Plasmids are perceived as mobile genetic elements that exist extra-
chromosomally and occasionally carry accessory genes that confer an
advantage to their host in its ecological niche. They are thus thought to
play an important evolutionary role in microbial communities by laterally
introducing genes and traits into microbial genomes. Plasmids are
important vehicles for rapid adaptation of bacterial populations to
changing environmental conditions. The genetic variation generated by
plasmid carriage within populations ensures the robustness towards
environmental change. Plasmid-mediated gene transfer plays an
important role not only in the mobilization and dissemination of antibiotic
resistance genes but also in the spread of degradative pathways and
pathogenicity determinants of pathogens.
Despite their importance, technical obstacles still limit the plasmid
study. Plasmid DNA sequencing is rapidly becoming a standard
approach to increase our understanding of the genetic diversity and
evolutionary history of plasmids. It not only helps to define the molecular
events that took place during the evolution of these plasmids, but also
give us a more complete overview of the enormous collection of
accessory genes encoded on plasmids. And comparative plasmid
sequence analysis has provided insights into the evolution of plasmids
and their relatedness, their modular structure and the existence of hot
spots for the insertion of accessory genes.
Key Features and Advantages
Complete validation of known plasmids
Full length sequencing of unkown plasmid
Complete characterization of plasmid genomes
No reference sequence required
Accurate assembly
Cost-effective high-throughput sequencing
High quality data & fast turnaround
Project Workflow
