Course Expectation and Rule Lab 8 - Biology I Cells | BIO 1011, Lab Reports of Biology

Material Type: Lab; Class: Biology I: Cells; Subject: Biology; University: Saint Joseph's University; Term: Unknown 1989;

Typology: Lab Reports

Pre 2010

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Course Information p.4
Bio 1012 Course Expectations & Rules
Major Course Objectives:
Biology 1011/1012 studies the structure and function of representative prokaryotic and eukaryotic cells.
By the end of this course you will:
have a basic understanding of the chemical composition of cells, the principles of enzyme-catalyzed
reactions, and energy-producing and biosynthetic reactions
In lab, students will develop their understanding in this area by examining the different responses of yeast to
specific sugars, examining what factors affect enzyme activity, determining the Vmax and Km for one substrate
of an enzyme, and separating the membrane-associated and cytoplasmic proteins of blood cells.
Students will be assessed in written lab reports for the yeast and enzyme labs and a workup with graph for the
protein lab. Understanding concepts, calculations, and approaches will also be accessed in a practical exam.
know the structure and function of cellular membranes, organelles, the cytoskeleton, and have an
understanding of cell signaling and communication
In lab, students will supplement lecture material on membranes & membrane transport by learning about the
process of osmosis through a computer simulation to examine the osmotic responses of model cells. Our investi-
gation of cell size will also allow students to become more familiar with cell structure and the organelles as seen
in micrographs. Student will also work with isolated choroplasts and an important process in these organelles.
Students will be assessed in a written lab report for the photosyntheis lab and in a practical exam. A worksheet
for diffusion and osmosis concepts regarding movement of water and solutes across membrane is graded. Some
student teams may present on organelle sizes as part of their team project in cell measurements.
gain experience in basic, bench-top laboratory skills in cell biology, know how to collect data and
represent it graphically, and be able to analyze data using statistical software.
Each week students will continue to examine cells and cell processes using laboratory approaches. Students will
collect cell size measurements using a variety of techniques and analyze the data sets produced using SPSS.
They will be introduced to and use basic techniques of measuring volumes, absorbance, masses, and
dimensions and of preparing reaction mixtures and solutions. Through instruction and worksheet assignments
students will learn about units and unit conversions. Students will construct graphs both on linear and logarithmic
axes and learn about the use of standard curves. Computer-assisted graphing and regression will also be
introduced through a tutorial for the enzyme lab. Students will be required to maintain a notebook record of their
results.
Students will be assessed in written lab reports, worksheet/graph assignments, a team presentation of their
statistical analysis for the cell size project, a notebook grade, and by practical exams. Students may be required
to individually demonstrate the use of equipment, collection of data, and ability to read a graph at the practical.
know how to write a laboratory report in the form of a scientific paper, and have experience in
conducting and presenting an independent experiment as a team
In lab, students will be introduced to scientific format and will produce two written lab reports in scientific format.
Instruction in format is aided by providing a large manual appendix on reports and “pointsheets”. Pointsheets
provide structure and tips for construction of the report and provide a framework for instructor feedback about
what portions need improvement. Student teams will design and conduct their own experiment. Teams will work
together to produce a symposium abstract that will be displayed in a mini-symposium at the conclusion of the
semester.
Reports will be assessed by grading (grade reflects both format and content). Team abstracts will be graded for
a team grade.
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Bio 1012 Course Expectations & Rules

Major Course Objectives:

Biology 1011/1012 studies the structure and function of representative prokaryotic and eukaryotic cells. By the end of this course you will:

  • have a basic understanding of the chemical composition of cells, the principles of enzyme-catalyzed reactions, and energy-producing and biosynthetic reactions

In lab, students will develop their understanding in this area by examining the different responses of yeast to specific sugars, examining what factors affect enzyme activity, determining the Vmax and Km for one substrate of an enzyme, and separating the membrane-associated and cytoplasmic proteins of blood cells.

Students will be assessed in written lab reports for the yeast and enzyme labs and a workup with graph for the protein lab. Understanding concepts, calculations, and approaches will also be accessed in a practical exam.

  • know the structure and function of cellular membranes, organelles, the cytoskeleton, and have an understanding of cell signaling and communication

In lab, students will supplement lecture material on membranes & membrane transport by learning about the process of osmosis through a computer simulation to examine the osmotic responses of model cells. Our investi- gation of cell size will also allow students to become more familiar with cell structure and the organelles as seen in micrographs. Student will also work with isolated choroplasts and an important process in these organelles.

Students will be assessed in a written lab report for the photosyntheis lab and in a practical exam. A worksheet for diffusion and osmosis concepts regarding movement of water and solutes across membrane is graded. Some student teams may present on organelle sizes as part of their team project in cell measurements.

  • gain experience in basic, bench-top laboratory skills in cell biology, know how to collect data and represent it graphically, and be able to analyze data using statistical software.

Each week students will continue to examine cells and cell processes using laboratory approaches. Students will collect cell size measurements using a variety of techniques and analyze the data sets produced using SPSS. They will be introduced to and use basic techniques of measuring volumes, absorbance, masses, and dimensions and of preparing reaction mixtures and solutions. Through instruction and worksheet assignments students will learn about units and unit conversions. Students will construct graphs both on linear and logarithmic axes and learn about the use of standard curves. Computer-assisted graphing and regression will also be introduced through a tutorial for the enzyme lab. Students will be required to maintain a notebook record of their results.

Students will be assessed in written lab reports, worksheet/graph assignments, a team presentation of their statistical analysis for the cell size project, a notebook grade, and by practical exams. Students may be required to individually demonstrate the use of equipment, collection of data, and ability to read a graph at the practical.

  • know how to write a laboratory report in the form of a scientific paper, and have experience in conducting and presenting an independent experiment as a team

In lab, students will be introduced to scientific format and will produce two written lab reports in scientific format. Instruction in format is aided by providing a large manual appendix on reports and “pointsheets”. Pointsheets provide structure and tips for construction of the report and provide a framework for instructor feedback about what portions need improvement. Student teams will design and conduct their own experiment. Teams will work together to produce a symposium abstract that will be displayed in a mini-symposium at the conclusion of the semester.

Reports will be assessed by grading (grade reflects both format and content). Team abstracts will be graded for a team grade.

Attendance Policy

You cannot "skip lab". Two unexcused lab absences will result in a FA for the entire course. Students must attend all labs. You should attend only your scheduled section (Monday, Tuesday, etc.); crossover is not allowed. It is your responsibility to contact your instructor (or Dr. Ratterman, if necessary) if you will miss or do miss a lab for valid reasons. Make-up dates will be for the same Lab# week (some weeks’ lab runs M-F & others it is W-T, see schedule for days of same lab#). Your scheduled makeup lab might be earlier in the week than your missed lab. Practicals that are set up during one week only so if there is a valid reason you miss, attend one of the other practicals ASAP. Student athletes should give a copy of their team’s schedule to their teacher ahead of time and indicate what days they will miss and which other lab you will attend the same calendar week. It is your responsibility to talk with your coach to arrange reciprocal misses of practices, so you can attend the full make-up lab for those weeks you miss your own lab for games/travel to games. Holidays. Do not schedule extra days to your holidays. Friday lab meets for the full period before Fall Break and Monday and Tuesday labs run their usual full times before Thanksgiving. Yes they do.

Calculators

Handsprings, Palm Pilots or other PDAs, cell phones, and hand-held computers are not allowed at practicals. Graphing/Programming calculators are also prohibited. Buy a cheap calculator to bring with you!! You need only simple functions for these lab exams.

Course Grade

You receive one grade for BIO 1011 that includes BIO 1012 work. The lab is worth 25% of your grade.

Lecture Tests 4x100+120 = 520 65% Lecture Quizzes 8x10 = 80 10% Lab 200 25% Total 800

Lab Grade breakdown

Assignment Value each Total value Two practicals Each 30 pts 60 pts Two full lab reports - Yeast and Photosynthesis Each 30 pts 60 pts EK Assigment – graphs, values, results, questions Instead of full lab report will focus on correct completion of graphs and est of Km/Vmax and examination of results

20 20 pts

Team Presentation – Stats on Cell Measurements 10 10 Team Abstract – Team’s Own Lab 10 10 Calcs Worksheets – average of three worksheets units wks 1, units wk 2, microscopy practice lab 1a

Each corrected out of 10, averaged

Graphs – average of three graphing assignments Bradford, Spectrum, Protein SDS-PAGE

Each corrected out of 10, averaged

Concept worksheets– ave of three worksheets Dilutions, diffusion, osmosis

Each corrected out of 10, averaged

Draft Checks & Lab Notebook Checks 1-2 pts each 10 TOTAL 200

Your lecture instructor will assign final course grades. Your lab instructor will give your lecture instructor your lab grade as points out of 200 pts. To help you keep track of how you are doing, your lab instructor may put a letter grade on assignments according to the straight scale: 90-100 A, 80- 89 B, 70-79 C, 60-69 D, <60 F. At any time, you can tell how well you are doing in lab by adding up the total points you have received and dividing this by the total possible points. Remember that there are also minus (-) and plus (+) ranges for each grade level for course grades. Do not expect any curves or adjustments; work to get the grade you want. See lecture Course Expectations for the grading scale.

Academic Honesty Policy

Cheating on a lab exam will result in a zero for that exam. Plagiarism in written assignments will result in a zero for that assignment. Teams will share data but each individual is expected to author his/her own lab report. Any student caught cheating for a second time will receive an F for the course. All incidents of cheating will be reported to the Office of Academic Records. Never copy from any source -- including your lab manual or textbook! Even when working with your team while completing a worksheet in class, the answers, observations, and calculations you write on your sheet should be your own work -- working together does NOT mean one person doing the work and the others copying. A true scientist is proud of his/her own integrity and follows a code of honesty about recording and reporting results. You start building your reputation now. See your student catalog for more information on SJU Academic Honesty Policy.

PLAGIARISM WARNING

1. Do not copy sentences directly from your textbook, your lab manual, or any other reference.

Always use your own words. Changing the "the"s and "that"s while keeping the rest of the sentence or just

chopping off part of the sentence is not sufficient -- RESTATE IDEAS IN YOUR OWN WORDS. If you have trouble

rewording, you need to read more so that you understand what you are talking about to be able to explain it in your

own words. Don't worry about sounding "professional" -- simple, direct language is often best. Do not quote

material in lab reports ; this is not part of the format. The whole report should be in your own words.

2. Do not copy from any other student's lab report or from old lab reports. You may start to work on

your results with your lab partners, but you must author your own report. Members of a lab group should NOT

have identically-worded RESULTS sections. Do not work too closely with another person so that your report texts

are parallel and similarly-worded. Labs change from year to year in both major and subtle ways and your instructor

is watching out for copiers of old reports - you will get caught. Don't accept or ask for old texts. Because

calculations and graphing are work you have to do for your lab report, you must do this yourself -- never copy

another's calculations or make replicas of their graphs (THAT is also plagiarism). Don't let anyone copy your work

either; this is also an act of academic dishonesty.

3. Do not photocopy or copy a picture, table or figure from a reference and include it in your report.

Report only your own results. Any schematic diagrams you wish to include should be your own construction,

perhaps adapted from a published source (you would cite that source e.g. "Adapted from Smith (1997)").

4. Do not request, accept, or give an electronic copy of any lab report or assignment text, table or

graph from another student. Even though lab teams share data, each individual student is to type in and

construct any and all tables and graphs in their own report. If the assignment requires you to turn in an electronic

copy or print-out of a computer-generated graph or table, submitting a version that was or was derived from another

student's file is cheating and both students will receive a zero for that assignment -- the giver and the receiver.

Keep your work to yourself.

5. Do not copy answers or calculations off your teammates' work in class. You may share raw data

but you should work the calculations yourself. If your teammate has prepared a slide you may certainly look in the

microscope and see it, but do not copy their observation statement word for word for your worksheet! Look at the

specimen and make your own observation. Teams may share collected data: but, author any written assignments

for class yourself.

EACH OF THE ABOVE (FIRST OFFENSE) WILL RESULT IN A GRADE OF ZERO FOR THAT LAB REPORT OR ASSIGNMENT

OR A COURSE "F". A SECOND OFFENSE WILL RESULT IN A GRADE OF "F" FOR THE COURSE.

Core Lab Rules

  1. Come to lab prepared : read the lab ahead of time. Bring your observation notebook, lab manual, calculator, a ruler, pen or pencil.
  2. Lab is scheduled for three hours and students will not be dismissed early. If you have completed your lab experiment, work on your graphs and calculations. If you have completed your graphs and calculations, work on the text of your next report, review lab procedures & equipment, or ask questions about lab material you are having difficulty with. Any additional time after experiments is not for socializing, it is for data analysis. You don’t need a computer to write a sentence for your report, try pencil & paper. Do not schedule appointments that conflict with lab times.
  3. If the lab takes longer than 3 hours, it is your responsibility to stay until lab is finished. If the experimental procedure is running longer than 3 hrs, you are responsible for staying to finish the lab. This is common lab rule in university laboratories and nothing worth whining about. Some labs may require a team member to come in at other time; it is your team's responsibility to make sure that this work is done at the proper time. 4. Work with, not against, your lab team. 3-person lab teams will be chosen the first or second day of lab. You will share responsibility for completing each lab with your teammates. Treat your teammates with respect, share the equipment at your bench, and do not hurry or harass any of your team members. No one gets out early so don't be impatient. Teamwork is required during lab but remember you will author your own lab report individually so make certain you have a copy of all your team's data before you leave lab.
  4. For your own safety:
    • No eating, drinking, chewing gum, or applying lipstick/lip balm in lab.
    • Wash your hands before leaving the lab room. Make this a habit.
    • Wear closed shoes for all biology labs. Do not wear sandals or open-toed shoes.
  5. Throw your trash into the appropriate container. Sinks are NOT wastebaskets! Trash cans (for garbage) and labeled GLASS WASTE bins (for glass ONLY) are in the room. All glass waste or sharps should be put into GLASS WASTE, never in the regular trash. Do not throw regular trash (kim-wipes, etc) into the GLASS WASTE container.In addition, DRY WASTE, GLASS WASTE, Contaminated Waste and/or LIQUID WASTE containers may be on your bench for some experiments. Recycling bins are available in the hallway; please put paper waste in blue bin (no postits or sharpie ink though). NEVER stick gum on any surface in the lab room – wrap gum in a small piece of paper and place in the trash.
  6. Handle lab equipment and materials responsibly. If you are unsure about the use of a piece of equipment please ask your instructor or TA (they are here to help you). If you accidentally break something, please let us know so we can see that it is repaired or replaced before the next lab.
  7. NO VANDALISM ALLOWED. Do not doodle onto the lab benches, scratch into any lab furniture, or any way destroy or damage any portion of the lab furniture or equipment. This lab was brand new in August 2005 so it is still our baby and we need to take care of it. If you have no respect for the new lab room, please do us a favor and take a different class.