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Material Type: Lab; Class: Biology I: Cells; Subject: Biology; University: Saint Joseph's University; Term: Unknown 1989;
Typology: Lab Reports
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Biology 1011/1012 studies the structure and function of representative prokaryotic and eukaryotic cells. By the end of this course you will:
In lab, students will develop their understanding in this area by examining the different responses of yeast to specific sugars, examining what factors affect enzyme activity, determining the Vmax and Km for one substrate of an enzyme, and separating the membrane-associated and cytoplasmic proteins of blood cells.
Students will be assessed in written lab reports for the yeast and enzyme labs and a workup with graph for the protein lab. Understanding concepts, calculations, and approaches will also be accessed in a practical exam.
In lab, students will supplement lecture material on membranes & membrane transport by learning about the process of osmosis through a computer simulation to examine the osmotic responses of model cells. Our investi- gation of cell size will also allow students to become more familiar with cell structure and the organelles as seen in micrographs. Student will also work with isolated choroplasts and an important process in these organelles.
Students will be assessed in a written lab report for the photosyntheis lab and in a practical exam. A worksheet for diffusion and osmosis concepts regarding movement of water and solutes across membrane is graded. Some student teams may present on organelle sizes as part of their team project in cell measurements.
Each week students will continue to examine cells and cell processes using laboratory approaches. Students will collect cell size measurements using a variety of techniques and analyze the data sets produced using SPSS. They will be introduced to and use basic techniques of measuring volumes, absorbance, masses, and dimensions and of preparing reaction mixtures and solutions. Through instruction and worksheet assignments students will learn about units and unit conversions. Students will construct graphs both on linear and logarithmic axes and learn about the use of standard curves. Computer-assisted graphing and regression will also be introduced through a tutorial for the enzyme lab. Students will be required to maintain a notebook record of their results.
Students will be assessed in written lab reports, worksheet/graph assignments, a team presentation of their statistical analysis for the cell size project, a notebook grade, and by practical exams. Students may be required to individually demonstrate the use of equipment, collection of data, and ability to read a graph at the practical.
In lab, students will be introduced to scientific format and will produce two written lab reports in scientific format. Instruction in format is aided by providing a large manual appendix on reports and “pointsheets”. Pointsheets provide structure and tips for construction of the report and provide a framework for instructor feedback about what portions need improvement. Student teams will design and conduct their own experiment. Teams will work together to produce a symposium abstract that will be displayed in a mini-symposium at the conclusion of the semester.
Reports will be assessed by grading (grade reflects both format and content). Team abstracts will be graded for a team grade.
You cannot "skip lab". Two unexcused lab absences will result in a FA for the entire course. Students must attend all labs. You should attend only your scheduled section (Monday, Tuesday, etc.); crossover is not allowed. It is your responsibility to contact your instructor (or Dr. Ratterman, if necessary) if you will miss or do miss a lab for valid reasons. Make-up dates will be for the same Lab# week (some weeks’ lab runs M-F & others it is W-T, see schedule for days of same lab#). Your scheduled makeup lab might be earlier in the week than your missed lab. Practicals that are set up during one week only so if there is a valid reason you miss, attend one of the other practicals ASAP. Student athletes should give a copy of their team’s schedule to their teacher ahead of time and indicate what days they will miss and which other lab you will attend the same calendar week. It is your responsibility to talk with your coach to arrange reciprocal misses of practices, so you can attend the full make-up lab for those weeks you miss your own lab for games/travel to games. Holidays. Do not schedule extra days to your holidays. Friday lab meets for the full period before Fall Break and Monday and Tuesday labs run their usual full times before Thanksgiving. Yes they do.
Handsprings, Palm Pilots or other PDAs, cell phones, and hand-held computers are not allowed at practicals. Graphing/Programming calculators are also prohibited. Buy a cheap calculator to bring with you!! You need only simple functions for these lab exams.
You receive one grade for BIO 1011 that includes BIO 1012 work. The lab is worth 25% of your grade.
Lecture Tests 4x100+120 = 520 65% Lecture Quizzes 8x10 = 80 10% Lab 200 25% Total 800
Assignment Value each Total value Two practicals Each 30 pts 60 pts Two full lab reports - Yeast and Photosynthesis Each 30 pts 60 pts EK Assigment – graphs, values, results, questions Instead of full lab report will focus on correct completion of graphs and est of Km/Vmax and examination of results
20 20 pts
Team Presentation – Stats on Cell Measurements 10 10 Team Abstract – Team’s Own Lab 10 10 Calcs Worksheets – average of three worksheets units wks 1, units wk 2, microscopy practice lab 1a
Each corrected out of 10, averaged
Graphs – average of three graphing assignments Bradford, Spectrum, Protein SDS-PAGE
Each corrected out of 10, averaged
Concept worksheets– ave of three worksheets Dilutions, diffusion, osmosis
Each corrected out of 10, averaged
Draft Checks & Lab Notebook Checks 1-2 pts each 10 TOTAL 200
Your lecture instructor will assign final course grades. Your lab instructor will give your lecture instructor your lab grade as points out of 200 pts. To help you keep track of how you are doing, your lab instructor may put a letter grade on assignments according to the straight scale: 90-100 A, 80- 89 B, 70-79 C, 60-69 D, <60 F. At any time, you can tell how well you are doing in lab by adding up the total points you have received and dividing this by the total possible points. Remember that there are also minus (-) and plus (+) ranges for each grade level for course grades. Do not expect any curves or adjustments; work to get the grade you want. See lecture Course Expectations for the grading scale.
Cheating on a lab exam will result in a zero for that exam. Plagiarism in written assignments will result in a zero for that assignment. Teams will share data but each individual is expected to author his/her own lab report. Any student caught cheating for a second time will receive an F for the course. All incidents of cheating will be reported to the Office of Academic Records. Never copy from any source -- including your lab manual or textbook! Even when working with your team while completing a worksheet in class, the answers, observations, and calculations you write on your sheet should be your own work -- working together does NOT mean one person doing the work and the others copying. A true scientist is proud of his/her own integrity and follows a code of honesty about recording and reporting results. You start building your reputation now. See your student catalog for more information on SJU Academic Honesty Policy.
PLAGIARISM WARNING
Core Lab Rules