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Cytoplasm: Cell differentiation: Cells and extracellular matter together compromise all the tissue that make up the organ of multicellular animals. The first zygotic cellular division produce cells called blastomeres. Embryonic stem cells are explanted to tissue culture cells of the inner cell mass. Differentiation is where cells differently express their set of genes that meditate specific cytoplasmic activity becoming very effective in their cytoplasmic activity and changing their shape accordingly. Plasma membrane: Plasma membrane contains phospholipids, cholesterol, and proteins that contain oligosaccharides chains covalently linked to many phospholipid and protein molecules. This membrane functions as a selective barrier regulating the passage of materials into and out of the cell and facilitate the transport of specific molecules. Important role is to keep constant the ion content of cytoplasm. Carry out a number of signaling and specific recognition functions, plays a key role in interactions of the cells with its environment. Integrins are certain plasma membrane proteins that is linked to both cytoskeleton and extracellular matrix components and allows exchange of influences in both directions, between the cytoplasm and materials in the extracellular matrix. Membranes phospholipids are amphipathic , consisting of two non-polar long chain fatty acids linked to a charged polar head that bears a phosphate group Glycolipids include oligosaccharide chains that extend outward from the cell surface. Glycocalyx is a delicate cell surface coating on glycolipids. Is important for cell to cell interaction, for cellular migration. They have receptors,
antigenicity and monological importance as well. They are involved in signal transmission as well. Integral proteins are incorporated directly within the lipid bilayer. Peripheral proteins are bound to one of the two membrane surfaces, have function such as enzymatic action, activating different quantities of the cell, signal transmission. Multipass protein are polypeptide chains of many integral proteins that span the membrane from one side to the other. Fluid mosaic model describes the structure of the plasma membrane as mosaic of components, including phospholipids, cholesterol, proteins and carbohydrates. Lipid rafts they are regions of plasma membrane , that contain components of large enzyme complexes that are usually less mobile especially involved in the transduction of signals from outside the cells, they have higher conc of cholesterol and saturated fatty acids reducing lipid fluidity. They have the least mobility and the least fluidity and they have high level of receptors. Functions of plasma membrane: Physical barrier: Phospholipid bilayer separates substances inside and outside the cell. Selective permeability: Regulates entry and exit of ions, nutrients, and waste molecules through the membrane Electrochemical gradient: establishes and maintains an electrical charge difference across the plasma membrane. Communication: contains receptors that recognize and responds to molecular signals. Passive and active transport: o Passive transport: movement that does not require energy against concentration gradient. o Active transport: movement of dissolved molecules into and out of the cell through the cell membrane. Against the conc gradient and it uses energy. Transmembrane protein and membrane transport.
containing the molecules to be secreted fuses with the plasma membrane, releasing the contents in the extracellular space. Exocytosis of macromolecules made by cells occurs in 2 ways: Constitutive secretion: used for products that are released from cells continuously as soon as synthesis is complete. Regulated secretion: occurs in response to signals coming to the cells, for e.g. release of digestive enzymes from pancreatic cells. Process of membrane movement and recycling is called membrane trafficking, occurs continuously in most cells. Small vesicles released are called exosomes which fuse with other cells transferring their contents and membranes. Signal reception and transduction: Many adjacent cells form communicating gap junctions that couple the cells and allow exchange of ions and small molecules. Route from signal molecule from source to target provide one to categorize the signaling process: Endocrine signaling: signal molecules carried in the blood from their sources to target cells throughout the body. Pancrine signaling: chemical ligand diffuses in extracellular fluid but is rapidly metabolized so that its effect is only local on target cells near its source. Synaptic signaling: neurotransmitters act on adjacent cells through special contract areas called synapses. Autocrine signaling: signals bind receptors on the same cell that produced the messenger molecule. Juxtacrine signaling: the signaling molecules are cell membrane bound proteins which bind surface receptors of the target cells when the two cells make direct physical contact. Receptors for hydrophilic signaling molecules including polypeptide hormones are usually transmembrane proteins in the plasmalema of the target cells.
Channel linked receptors: open associated channels upon ligand binding to promote transfer of molecules or ions across the membrane. Enzymatic receptors: ligand binding induces catalytic activity in associated peripheral proteins. G- Protein coupled receptors: ligand binding associate G protein which the bind the guanine nucleotide and are released to activate other cytoplasmic protein. Ligand binding of such receptors begins the process of signal transduction by activating a series of intermediary enzymes downstream to produce changes in the cytoplasm or nucleus or both Activation of kinases can activate various cytoplasmic proteins, amplifying the signal. Activated G proteins target ion channels or other membrane bound effectors that also propagate the signal into the cell. Enzyme adenylyl cyclase generates large quantities of second messenger molecules such as cyclic adenosine.