DNA Profiling Worksheet, Study notes of Biology

DNA Profiling Worksheet ... Step in DNA fingerprinting that “unzips” the ... The entire process used to get a DNA fingerprint takes less than 2 hours.

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Biology I Name____________________
DNA Profiling Worksheet
_____1. Copying process used to create many
of the same cells
A. Autoradiograph
B. Cloning
C. Electrophoresis
D. “Junk” DNA
E. Probe
F. Restriction enzyme
G. Southern Blot
H. VNTRs
_____2. Multiple copies of short nucleotide sequences
repeated one after another
_____3. Chemical used to cut DNA at certain points
_____4. Part of DNA that does not code for proteins
_____5. Method for separating pieces of DNA by size
_____6. Step in DNA fingerprinting that “unzips” the
DNA and transfers it from the gel to a nylon
membrane
_____7. Final product of the DNA profiling which shows
the DNA “fingerprint”
True or False
_____8. The entire process used to get a DNA fingerprint takes less than 2 hours.
_____9. Most of our DNA consists of non-coding “junk”.
_____10. VNTRs are not inherited like genes for our traits.
_____11. DNA profiling involves analysis of ALL the variable DNA sequences.
_____12. Like blood typing, DNA can only be used to prove someone innocent, not to prove someone guilty.
_____13. Only some people have “junk” DNA with variable numbers of tandem repeated nucleotide
sequences.
_____14. Within the portion of our DNA that codes for proteins, people are more alike than different.
_____15. DNA samples from a crime scene can often be contaminated with foreign DNA from bacteria, etc.
_____16. Although one VNTR location may be satisfactory for determining paternity, 4 or 5 are often needed
to convict a suspect of a crime.
_____17. While every band on the DNA fingerprint of a suspect must match that from the DNA crime
evidence, every band on a child’s DNA fingerprint does not have to match his father’s.
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Biology I Name____________________

DNA Profiling Worksheet

_____1. Copying process used to create many of the same cells A. Autoradiograph B. Cloning C. Electrophoresis D. “Junk” DNA E. Probe F. Restriction enzyme G. Southern Blot H. VNTRs _____2. Multiple copies of short nucleotide sequences repeated one after another _____3. Chemical used to cut DNA at certain points _____4. Part of DNA that does not code for proteins _____5. Method for separating pieces of DNA by size _____6. Step in DNA fingerprinting that “unzips” the DNA and transfers it from the gel to a nylon membrane _____7. Final product of the DNA profiling which shows the DNA “fingerprint”

True or False

_____8. The entire process used to get a DNA fingerprint takes less than 2 hours. _____9. Most of our DNA consists of non-coding “junk”. _____10. VNTRs are not inherited like genes for our traits. _____11. DNA profiling involves analysis of ALL the variable DNA sequences. _____12. Like blood typing, DNA can only be used to prove someone innocent, not to prove someone guilty. _____13. Only some people have “junk” DNA with variable numbers of tandem repeated nucleotide sequences. _____14. Within the portion of our DNA that codes for proteins, people are more alike than different. _____15. DNA samples from a crime scene can often be contaminated with foreign DNA from bacteria, etc. _____16. Although one VNTR location may be satisfactory for determining paternity, 4 or 5 are often needed to convict a suspect of a crime. _____17. While every band on the DNA fingerprint of a suspect must match that from the DNA crime evidence, every band on a child’s DNA fingerprint does not have to match his father’s.

Biology I Name____________________

  1. Put these steps in the correct order from 1-7: ____ expose to x-ray film ____ isolate the DNA ____ use electrophoresis ____ “Southern Blot” ____ cut DNA using restriction enzymes ____ result is an autoradiograph ____ add radioactively tagged probe
  2. Which child belongs to this couple? ___________ Wife Husband Child 1 Child 2 Child 3
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  1. Which suspect is guilty? ___________ Evidence Suspect 1 Suspect 2 Suspect 3
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