TERM 1
Central Dogma of Biology
DEFINITION 1
Describes the flow of genetic information within a biological
system. DNA (RNA polymerase) > RNA > (ribosome) Protein
TERM 2
DNA Replication
DEFINITION 2
The process of making an identical copy of a section ofdouble
strandedDNA, using existing DNA as a template for the
synthesis of new DNA strands.In humans and other
eukaryotes,replication occurs in the cell nucleus.Happens
during S phase before mitosis.
TERM 3
DNA Replication
Initiation
DEFINITION 3
1. DnaA binds to ori and opens up the DNA2. Two DNA helicase
(DnaB) proteins are loadedontothe st rand in opposite directions.3.
Helicase loading protein (DnaC) helps load helicase.4. Primase
(DnaG) is then added to start the rep lication by synthesising a
shortcomplementaryRNA sequence .5. DNA polymerase III then
binds and replicates the DNA.6. The s liding clamp (DnaN) stops
DNA polymerase III from falling of.7. Sin gle stranded binding
proteins prevent ssDNA from anneali ng.
TERM 4
DNA Replication
DEFINITION 4
Labelled Diagram
TERM 5
DNA Polymerase III
DEFINITION 5
Shaped roughly like a clasping hand
Space between fingers and thumb ri ght size for DNA helix
Correct positioning of incoming deoxy nucleoside triphosphate
(bunch of DNA), tightens polymerase
Initiating nucleotide addition reaction
Dissociation of pyrophosphate causes loosening
Translocation of DNA so DNA Polyme rase III ready to receive
next deoxynucleoside triphosphate
