Dragon Genetics Lab: A Hands-On Exploration of Mendelian Inheritance, Study notes of Genetics

Dragons are an extremely endangered species and researchers are interested in documenting the genetics of the species and starting a captive breeding program in ...

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2021/2022

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DRAGON GENETICS LAB
BACKGROUND
Dragons are an extremely endangered species and researchers are interested in
documenting the genetics of the species and starting a captive breeding program in order to
increase the dragon population. Each student will become a surrogate dragon parent. Dragons
have five pairs of homologous chromosomes (2n = 10), including a single pair of sex
chromosomes and four pairs of autosomes. Students will analyze the chromosomes of their
dragon and determine its genotype and resulting phenotype. This information will be recorded on
the following pages to aid in future dragon research and captive breeding programs.
Next, students will seek out another student with a dragon that is of a different gender
then their own and allow their dragons to mate. Dragons, like all other sexually reproducing
organisms, produce gametes by meiosis. During meiosis each dragon will produce haploid
gametes containing only one allele from each gene. Meiosis will occur according to the same
principles of segregation and independent assortment that are typically observed in meiosis. The
alleles for each gene on each inherited chromosome will be documented. This information will
then be used to determine the genotype of the baby dragon and predict its phenotype.
PROCEDURE
1. Each student must pick up five pairs popsicle stick chromosomes two of each color of
autosome, and two sex chromosomes.
2. For each pair of colored autosomes, and then for the pair of sex chromosomes, each parent
will randomly drop his or her chromosomes on the table. The side of the stick that is facing
up represents the combinations of alleles in your pet dragons genotype. Arrange the
chromosomes in front of you in homologous pairs (matching colors). Be sure to keep them
with the same side facing up for the rest of this lab or you will become extremely confused.
3. Look at the homologous pair of sex chromosomes and determine if your dragon is a male or
female. Record this information at the bottom of the data sheet.
4. Record the pairs of alleles from each pair of homologous chromosomes in the data chart
under parental genotype (If your dragon is female use the “mom” box. If your dragon is male
use the “dad” box.
5. The decoding table indicates the phenotypic effect of each gene. The trait produced by each
pair of parental alleles should be recorded in the data chart under parental phenotype.
Remember not all traits follow normal Mendelian inheritance patterns of strictly dominant or
recessive. Check for alternate inheritance patterns such as codominance, incomplete
dominance, pleiotropy, or polygenic inheritance at the bottom of the decoding table.
6. Before they can mate dragons must produce gametes through the process of meiosis. To
simulate the production of gametes by meiosis we will flip a coin. Heads, the gamete
contains the chromosome on the left in each homologous pair of chromosomes. Tails, the
gamete contains the chromosome on the right in each homologous pair of chromosomes.
You will need to flip a coin once for each pair of homologous chromosomes. Record the
alleles from each of the inherited chromosomes in the appropriate “alleles found in” column
of the data table. (If your dragon is male it produces sperm, if it is female it produces eggs)
7. Now that you know the genetic make-up of your dragon you are ready to have it mate with
another dragon. Find another dragon surrogate parent with a dragon whose genetic make-up
has been completed and is known to be of a different gender. Record the name of your
dragon mates surrogate parent at the bottom of the data sheet.
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DRAGON GENETICS LAB

BACKGROUND

Dragons are an extremely endangered species and researchers are interested in documenting the genetics of the species and starting a captive breeding program in order to increase the dragon population. Each student will become a surrogate dragon parent. Dragons have five pairs of homologous chromosomes (2n = 10), including a single pair of sex chromosomes and four pairs of autosomes. Students will analyze the chromosomes of their dragon and determine its genotype and resulting phenotype. This information will be recorded on the following pages to aid in future dragon research and captive breeding programs. Next, students will seek out another student with a dragon that is of a different gender then their own and allow their dragons to mate. Dragons, like all other sexually reproducing organisms, produce gametes by meiosis. During meiosis each dragon will produce haploid gametes containing only one allele from each gene. Meiosis will occur according to the same principles of segregation and independent assortment that are typically observed in meiosis. The alleles for each gene on each inherited chromosome will be documented. This information will then be used to determine the genotype of the baby dragon and predict its phenotype.

PROCEDURE

1. Each student must pick up five pairs popsicle stick chromosomes – two of each color of

autosome, and two sex chromosomes.

2. For each pair of colored autosomes, and then for the pair of sex chromosomes, each parent

will randomly drop his or her chromosomes on the table. The side of the stick that is facing up represents the combinations of alleles in your pet dragons genotype. Arrange the chromosomes in front of you in homologous pairs (matching colors). Be sure to keep them with the same side facing up for the rest of this lab or you will become extremely confused.

3. Look at the homologous pair of sex chromosomes and determine if your dragon is a male or

female. Record this information at the bottom of the data sheet.

4. Record the pairs of alleles from each pair of homologous chromosomes in the data chart

under parental genotype (If your dragon is female use the “mom” box. If your dragon is male use the “dad” box.

5. The decoding table indicates the phenotypic effect of each gene. The trait produced by each

pair of parental alleles should be recorded in the data chart under parental phenotype. Remember not all traits follow normal Mendelian inheritance patterns of strictly dominant or recessive. Check for alternate inheritance patterns such as codominance, incomplete dominance, pleiotropy, or polygenic inheritance at the bottom of the decoding table.

6. Before they can mate dragons must produce gametes through the process of meiosis. To

simulate the production of gametes by meiosis we will flip a coin. Heads, the gamete contains the chromosome on the left in each homologous pair of chromosomes. Tails, the gamete contains the chromosome on the right in each homologous pair of chromosomes. You will need to flip a coin once for each pair of homologous chromosomes. Record the alleles from each of the inherited chromosomes in the appropriate “alleles found in” column of the data table. (If your dragon is male it produces sperm, if it is female it produces eggs)

7. Now that you know the genetic make-up of your dragon you are ready to have it mate with

another dragon. Find another dragon surrogate parent with a dragon whose genetic make-up has been completed and is known to be of a different gender. Record the name of your dragon mates surrogate parent at the bottom of the data sheet.

8. Record the genetic make up of your partners dragon in the remaining parental genotype and

phenotype columns.

9. Record the alleles from the gamete it produced in the remaining “alleles found in” column.

10. Use the alleles listed for each of the parent dragon gametes to determine the genotype of your

dragon offspring. Record this in the data table in the “offspring genotype” column.

11. Compare the offspring genotype information to the decoding table to determine the

phenotype of your baby dragon for each of the traits. Record this information in the “offspring phenotype” column on the data table.

12. Draw and color a picture of your baby dragon on a blank piece of paper. Use the picture of

the various traits at the end of this lab to help with any unfamiliar traits. Label the genotype and the phenotype of each trait on the drawing QUESTIONS

Answer in complete sentences on a separate piece of paper.

1. State Mendel’s Law of Segregation in your own words. During this lab how did we simulate

the law of segregation? What accounts for segregation in the cells of living things?

2. State Mendel’s Law of Independent Assortment in your own words. Compare the way we

assorted alleles in this lab to the way Mendel believed alleles assort into gametes. Did every pair of alleles assort independently into gametes in this lab? Why or why not?

3. The gene for no fangs is dominant, yet most of the dragons have fangs. How did this

happen? What conclusion can you make about the frequency of the alleles for this gene relative to each other? [Hint. Look up achondroplasia in your textbook]

4. Explain the difference between incomplete dominance and codominance. Use examples from

your dragon.

5. Explain the difference between pleiotropy and polygenic inheritance. Use examples from

your dragon to demonstrate the difference.

6. What genes are more likely to be expressed in male dragons than female dragons? Explain!

( This assumes that the dominant and recessive alleles for each of these traits are equally likely in the dragon population )

7. Imagine that nondisjunction occurred during the production of the egg cell that produced your

dragon’s baby and resulted in no sex chromosome in the sperm cell. How would this change the phenotype of your dragons’ baby? Be specific for each trait that could possibly be affected.

8. Do you and your partners baby dragon have any traits that are not seen in the phenotype of

either parent? If so list them. Explain how it is possible for offspring to have phenotypes not seen in either parent.

Green Autosomes

Parental Genotypes Parental Phenotypes Allele Found in: Mom Dad Mom Dad Egg Sperm Offspring Genotype Offspring Phenotype

Red Autosomes

Parental Genotypes Parental Phenotypes Allele Found in: Mom Dad Mom Dad Egg Sperm Offspring Genotype Offspring Phenotype

Blue Autosomes

Parental Genotypes Parental Phenotypes Allele Found in: Mom Dad Mom Dad Egg Sperm Offspring Genotype Offspring Phenotype

Yellow Autosomes

Parental Genotypes Parental Phenotypes Allele Found in: Mom Dad Mom Dad Egg Sperm Offspring Genotype Offspring Phenotype

Sex Chromosomes

Parental Genotypes Parental Phenotypes Allele Found in: Mom Dad Mom Dad Egg Sperm Offspring Genotype Offspring Phenotype

Gender of your Dragon: ________________________

Dragons Mate’s Surrogate Parent Name (your partner): _________________

Gender of Your Dragons Baby: __________________