Embryogenesis and storage protein, Lecture notes of Botany and Agronomy

These notes cover the topic of Embryogenesis and Storage. The document includes complete explanations of plant embryogenesis, stages of embryo development, types of endosprem, seed formation, and storage proteins found in plants. Important concepts such as zygote formation, globular stage, heart-shaped embryo stage, mature embryo are covered. The notes are useful for B.Sc. Medical, B.Sc. Botany, Biotechnology, Life Sciences, and Agriculture students. They are especially helpful for semester examinations, competitive exams, and quick revision. The content is written in simple language with clear headings, definitions, examples, and short explanations for better understanding. Subject: Botany / Plant Biology / Plant Embryology Topic: Embryogenesis and Storage Proteins Course: B.Sc. Medical / Botany Honors / Life Sciences Level: Undergraduate Document Type: Handwritten/Class Notes with Diagrams Language: English Suitable For: University Exams, Assignments, Viva, and Revision Preparation

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2025/2026

Available from 05/29/2026

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Embryogenesis in Plants
Introduction
Plant Embryogenesis is the process by which plant embryos form and develop. The
Zygote produced after fertilization must undergo various cellular divisions and
differentiation to become a mature embryo. Or Embryogenesis is the process of
formation and development of an embryo from the zygote after fertilization. In
๏ฌ‚owering plants, it begins inside the ovule after syngamy and continues until a
mature embryo is formed within the seed.
The embryo later develops into a new plant during seed germination;
Process of Embryogenesis in Angiosperms
1. Formation of Zygote: ๏ฌrst stage of diploid life stort with fusion of haploid egg
and to form single celled zygote. Division at this stage give rise to small apical and
elongated basal cell.
โ—During double fertilization, one male gamete fuses with the egg cell.
โ—This fusion is called syngamy.
โ—The diploid cell formed is known as the zygote.
The zygote is the ๏ฌrst cell of the future embryo.
2. Resting Period of Zygote
โ—In many plants, the zygote undergoes a short resting phase.
โ—During this time:
oEndosperm develops ๏ฌrst.
oFood materials accumulate.
โ—After this, the zygote starts dividing.
Development of Embryo
A. Early Embryo Development
First Division of Zygote
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Embryogenesis in Plants Introduction Plant Embryogenesis is the process by which plant embryos form and develop. The Zygote produced after fertilization must undergo various cellular divisions and differentiation to become a mature embryo. Or Embryogenesis is the process of formation and development of an embryo from the zygote after fertilization. In flowering plants, it begins inside the ovule after syngamy and continues until a mature embryo is formed within the seed. The embryo later develops into a new plant during seed germination; Process of Embryogenesis in Angiosperms

  1. Formation of Zygote: first stage of diploid life stort with fusion of haploid egg and to form single celled zygote. Division at this stage give rise to small apical and elongated basal cell. โ— During double fertilization, one male gamete fuses with the egg cell. โ— This fusion is called syngamy. โ— The diploid cell formed is known as the zygote. The zygote is the first cell of the future embryo.
  2. Resting Period of Zygote โ— In many plants, the zygote undergoes a short resting phase. โ— During this time: o Endosperm develops first. o Food materials accumulate. โ— After this, the zygote starts dividing. Development of Embryo A. Early Embryo Development First Division of Zygote

โ— The zygote divides transversely into two unequal cells:

  1. Terminal cell (apical cell)
  2. Basal cell Functions โ— Terminal cell forms the main embryo. โ— Basal cell forms the suspensor. B. Formation of Suspensor

Suspensor โ— The basal cell divides repeatedly to form a filament-like structure called the suspensor. Functions of Suspensor

  1. Pushes the embryo into the nutritive endosperm.
  2. Transfers nutrients to the developing embryo.

โ— Embryo becomes spherical or globular. โ— Tissue differentiation begins. โ— Three primary meristems develop:

  1. Protoderm epidermis
  2. Procambium vascular tissues
  3. Ground meristem cortex and pith
  4. Heart-Shaped Stage: This stage forms rapid cell division in to regions either side of the future shoot apex. These two regions produce outgrowth that later will give rise to the Cotyledons and give the embryo bilateral symmetry. Two cotyledons start developing. Embryo becomes heart-shaped in dicots. Growth of shoot apex begins between cotyledons.
  5. Torpedo Stage: This stage forms a result of cell elongation throughout the embryo and further development to the cotyledons visible distinction between adaxial (front) and abaxial (back).

โ— Rapid elongation occurs. โ— Embryonal axis becomes distinct. โ— Root and shoot regions become clear.

  1. Mature Embryo: Toward the end of embryogenesis the embryo and seed loose water and become metabolically quiescent as they enter dormancy.

Monocot embryo differs from dicot embryo. Main Parts

  1. Scutellum โ€“ single cotyledon
  2. Coleoptile โ€“ sheath covering plumule
  3. Coleorhiza โ€“ sheath covering radicle Example: Maize Types of Embryogenesis
  1. Zygotic Embryogenesis โ— Embryo develops naturally from zygote after fertilization. โ— Occurs inside seeds.
  2. Somatic Embryogenesis

Importance โ— Clonal propagation โ— Artificial seed production โ— Genetic engineering Importance of Embryogenesis

  1. Helps in formation of new plant.
  2. Establishes root-shoot axis.
  3. Ensures continuity of species.
  4. Important in seed formation.
  5. Useful in agriculture and biotechnology. Vegetative and Seed Storage Proteins Storage proteins are reserve proteins that store nitrogen and amino acids for plant growth and development. They are mainly of two types:
  6. Vegetative Storage Proteins (VSPs)
  7. Seed Storage Proteins (SSPs)
  8. Vegetative Storage Proteins (VSPs) Vegetative storage proteins are proteins stored in vegetative parts of plants such as leaves, stems, tubers, and roots. Definition They are temporary reserve proteins accumulated in vegetative tissues and later mobilized for growth, flowering, and seed development. Characteristics โ— Found in non-seed tissues. โ— Accumulate during active growth. โ— Serve as nitrogen reserves. โ— Stored mainly in vacuoles. โ— Broken down when plant needs nutrients. Functions
  9. Store nitrogen and amino acids.
  10. Provide nutrients during flowering and seed formation.
  11. Help plants survive unfavorable conditions.
  12. Support regrowth after injury or dormancy. Examples โ— Patatin in potato tubers. โ— Sporamin in sweet potato. โ— VSP proteins in soybean leaves. Importance

โ— Help in nutrient recycling. โ— Important for perennial plants. โ— Useful in stress tolerance and regeneration.

  1. Seed Storage Proteins (SSPs) Seed storage proteins are reserve proteins deposited in seeds during embryogenesis and seed maturation. Definition They are proteins stored in cotyledons or endosperm that provide nutrition during seed germination. Characteristics โ— Accumulate in developing seeds. โ— Stored in protein bodies. โ— Rich source of nitrogen and amino acids. โ— Used during germination. Functions
    1. Supply nutrients to embryo.
    2. Provide amino acids for seedling growth.
    3. Maintain seed viability.
    4. Important for human and animal nutrition. Types of Seed Storage Proteins (a) Albumins โ— Water soluble. โ— Example: Legume proteins. (b) Globulins โ— Salt soluble. โ— Major proteins in legumes. โ— Example: Legumin and vicilin. (c) Prolamins โ— Alcohol soluble. โ— Found in cereals. โ— Example: Zein in maize, gliadin in wheat. (d) Glutelins โ— Soluble in dilute acids/alkali. โ— Example: Glutenin in wheat. Examples โ— Legumin in pea. โ— Zein in maize.