Food Spectroscopy Presentation, Slides of Inorganic Chemistry

Download Food Spectroscopy Presentation and more Slides Inorganic Chemistry in PDF only on Docsity!

Food Dye

Spectroscopy

Group 6: Ben, Liam, Ranvir, Roshini

Exploration Methodology

Evidence Conclusion

Table of contents

01 02

03 04

What is Spectroscopy

Image from https://www.atascientific.com.au/spectrometr y/

Light Spectroscopy: the study of how light interacts with matter, where different materials absorb and emit specific wavelengths of light

• Tested the wavelength and absorbance of food dyes that could be within a soft drink

Goal of our experiment

Replicate the Dye Spectra of a drink, so a generic version can be made

• Generic branded items must closely match the real product that

they are replicating to be competitive in a market

Replication of the soda dye spectra can be found by matching

wavelengths and absorbance of the original drink

Methodology

You can enter a subtitle here if you need it

Goal #2 Methodology

Goal #2: Plan an an investigation to test the relationship between dye solution

absorbance and dye concentration of the two (2) food dyes present in a colored

aqueous beverage

Using M1V1 = M2V2 equation, calculate the M2 for the two different dyes giving us

concentrations at different volumes (e.g. 2mL, 4mL, 6mL, 8mL)

Create different dilutions

Insert each concentration into spectrometer to read its absorbance

Determine line of best fit and r

Goal #3 Methodology

Goal #3: Produce a 10mL mock solution with the same color profile as a colored

beverage

Correctly calculate the concentration of the identified dyes in the unknown solution

using Beers Law (A = εbc) modeled by [Y = mX + b]

Use M1V1 = M2V2 to find volume of solution

Create unknown dye using calculated volume of each dye

Observe created dye and unknown beverage dye

Visual Methodology for Goal #1:

Procedure for goal #1:

2mL 4mL 6mL 8mL

Visual Methodology for Goal #2:

● We used these graphs to determine that blue

and yellow #5 dyes are used to create the

unknown substance

Visual Reasoning

for Goal #1:

Unknown Substance:

Yellow #5 (top) & Blue # (bottom)

Visual Reasoning for Goal #2:

Conclusion

Claim : By using the relationship between dye absorption and dye concentration, our team was able to replicate the color of the unknown aqueous solution.

Goal #1:

• The methodology used helped determine what dyes made up the unknown solution.
• As shown in the graph between wavelength v. absorbance, we were able to identify there were two peaks at 429.3nm and 631.5nm. How? - At a molecular level, as visible light strikes the matter, molecules will absorb certain wavelengths of light and transmit or reflect others based on the chemical bonds that are present. - Pigments and dyes absorb light in the 400-770nm
• It was concluded that the dyes present were Blue #1 and Yellow #