Immunology
&ELISAs
2013BIOMANWorkshop,MC3
LoriDodson,Ph.D.
ShowNTell
&freebies
atendof
workshop
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Immunology and ELISA Workshop: Resources and Commercial Kits, Exams of Immunology
Information about a workshop on Immunology and ELISAs, including a general overview, resources such as commercial kits from Bio-Rad, Edvotek, and Carolina, and online sites with simulations and animations. The hands-on component includes using kits from Bio-Rad and Edvotek for indirect ELISA and a quantitative assay. The document also covers miscellaneous topics like E-Gels and freebies.
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Immunology^ &
ELISAs
BIOMAN
Workshop,
MC
Lori
Dodson,
Ph.D.
Show
N^ Tell &^ freebies^ at^ end
of workshop
Disclaimers
I^
am
NOT
an
immunologist^ Killer
T^ cells
attacking
a^ cancer
cell
^ Nobody
is^ paying
me!
-^
The Perfect World
-^
The Real World
FLU^ COLD
CHICKENPOX
STOMACHUPSET
HELPME! HELP!
HELPME!
NCI’s
Understanding
The
Immune
System
Ab‐mediatedAcquired
Immunity
O^2 Response
ELISA
Indirect
Direct
Ab‐Ag Specificity
A^ Picture
is^ worth
a^1000
words!
Immunology/ELISA/HIV
http://www.biology.arizona.edu/immunology/activities/elisa/main.htmlhttp://www.biology.arizona.edu/immunology/activities/AIDS2003/main.htmlhttp://www.pbs.org/wgbh/nova/aids/textindex.htmlhttp://www.teachersdomain.org/
‐12/sci/life/gen/doubleimmunity/index.html
http://www.learner.org/channel/courses/biology/units/hiv/images.html
Commercial
Kits
Bio‐
Rad’s ELISA
Immuno Explorer
Kit
Edvotek’s AIDS
Kit
Carolina’s
ELISA
Simulation
Kit
Used
16 months
past
expiration;
great
results
(even
with
½^ volumes
so^ x
the^
rxns)
(Reagent
refill
Storage:
O ‐‐ 20
C
O (or 4 C^ for^1
month) Purple/Green
{no^ Ab/Ag} No^ pre
‐lab^ reagent
prep
16 pairs
of^ students No^ washing
steps
Protocol
Type of ELISA
Real-WorldApplication
I
Trackingoutbreaks ofdisease
by detecting antigens
HIV, SARS, Smallpox, anthrax
II^
Detectingantigens
Pregnancy, drugs,
GMO, BSE
III
Detectingantibodies inserum
Lyme disease,HIV, smallpox,West Nile virus
Bio‐Rad:
provides
detailed
information
with^ Kit
Example:
Pregnancy
Test
A^96
‐well
ELISA
plate
(^
cm^
x^12
cm)
?Results
determined?
PositiveControl
NegativeControl
Patient
A
Patient
B
Patient
C
AssayControl
O.
- The cutoff value indicating a positive result is 0.500• Optical densities of
^
0.499 are indeterminate and
OD^450 need to be retested
nm