Docsity
Docsity
Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity

Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan

Guidelines and tips
Guidelines and tips
Sell on Docsity
Sell on Docsity
Docsity AI
Docsity AI
Log inSign up

Prepare for your exams

Study with the several resources on Docsity

Find documents

Prepare for your exams with the study notes shared by other students like you on Docsity

Search for your university

Find the specific documents for your university's exams

Docsity AINEW

Summarize your documents, ask them questions, convert them into quizzes and concept maps

Explore questions

Clear up your doubts by reading the answers to questions asked by your fellow students

Earn points to download

Earn points by helping other students or get them with a premium plan

Share documents
download point icon20 Points

For each uploaded document

Answer questions
download point icon5 Points

For each given answer (max 1 per day)

All the ways to get free points
Get points immediately

Choose a premium plan with all the points you need

Study Opportunities

Choose your next study program

Get in touch with the best universities in the world. Search through thousands of universities and official partners

Community

Ask the community

Ask the community for help and clear up your study doubts

Free resources

Our save-the-student-ebooks!

Download our free guides on studying techniques, anxiety management strategies, and thesis advice from Docsity tutors

Lab Report: Using a Compound Light Microscope, Study notes of Biology

Abertay UniversityBiology

Instructions for using a compound light microscope, including handling procedures, preparing a wet mount, determining total magnification, and observing changes in the field of view. Students will learn how to properly use the microscope and observe specimens such as the letter 'e' and onion cells.

Typology: Study notes

2021/2022

Uploaded on 09/27/2022

jimihendrix
jimihendrix 🇬🇧

4.3

(15)

247 documents

1 / 5

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
Part I Introduction to the Microscope NAME_______________________________
"Micro" refers to tiny, "scope" refers to view or look at. Microscopes are tools used to enlarge images of
small objects so as they can be studied. The compound light microscope is an instrument containing two lenses,
which magnifies, and a variety of knobs to resolve (focus) the picture. Because it uses more than one lens, it is
sometimes called the compound microscope in addition to being referred to as being a light microscope. In this
lab, we will learn about the proper use and handling of the microscope.
Instructional Objectives
Demonstrate the proper procedures used in correctly using the compound light microscope.
Prepare and use a wet mount.
Determine the total magnification of the microscope.
Explain how to properly handle the microscope.
Describe changes in the field of view and available light when going from low to high power using the
compound light microscope
Explain why objects must be centered in the field of view before going from low to high power using
the compound light microscope.
Explain how to increase the amount of light when going from low to high power using the compound
light microscope.
Explain the proper procedure for focusing under low and high power using the compound light
microscope.
Materials
Compound microscope
Glass slides
Cover slips
Eye dropper
Beaker of water
The letter "e" cut from newsprint
Scissors
Procedures
I. Microscope Handling
1. Carry the microscope with both hands --- one on the arm and the other under the base of the
microscope.
2. One person from each group will now go over to the microscope storage area and properly transport
one microscope to your working area.
3. The other person in the group will pick up a pair of scissors, newsprint, a slide, and a cover slip.
4. Remove the dust cover and store it properly. Plug in the scope. Do not turn it on until told to do so.
5. Examine the microscope and give the function of each of the parts listed on the right side of the
diagram.
pf3
pf4
pf5

Partial preview of the text

Download Lab Report: Using a Compound Light Microscope and more Study notes Biology in PDF only on Docsity!

Part I Introduction to the Microscope NAME_______________________________

"Micro" refers to tiny , "scope" refers to view or look at. Microscopes are tools used to enlarge images of small objects so as they can be studied. The compound light microscope is an instrument containing two lenses , which magnifies, and a variety of knobs to resolve (focus) the picture. Because it uses more than one lens, it is sometimes called the compound microscope in addition to being referred to as being a light microscope. In this lab, we will learn about the proper use and handling of the microscope.

Instructional Objectives

 Demonstrate the proper procedures used in correctly using the compound light microscope.  Prepare and use a wet mount.  Determine the total magnification of the microscope.  Explain how to properly handle the microscope.  Describe changes in the field of view and available light when going from low to high power using the compound light microscope  Explain why objects must be centered in the field of view before going from low to high power using the compound light microscope.  Explain how to increase the amount of light when going from low to high power using the compound light microscope.  Explain the proper procedure for focusing under low and high power using the compound light microscope.

Materials

 Compound microscope  Glass slides  Cover slips  Eye dropper  Beaker of water  The letter "e" cut from newsprint  Scissors

Procedures

I. Microscope Handling

  1. Carry the microscope with both hands --- one on the arm and the other under the base of the microscope.
  2. One person from each group will now go over to the microscope storage area and properly transport one microscope to your working area.
  3. The other person in the group will pick up a pair of scissors, newsprint, a slide, and a cover slip.
  4. Remove the dust cover and store it properly. Plug in the scope. Do not turn it on until told to do so.
  5. Examine the microscope and give the function of each of the parts listed on the right side of the diagram.
  1. eyepiece or ocular
  2. body tube
  3. fine adjustment knob
  4. nosepiece
  5. high power objective
  6. low power objective
  7. diaphragm
  8. mirror (many microscopes have a light instead)
  9. base
  10. coarse adjustment
  11. arm
  12. stage clip
  13. inclination joint

Part II. Preparing a wet mount of the letter "e”.

  1. With your scissors cut out the letter "e" from the newspaper.
  2. Place it on the glass slide so as to look like (e).
  3. Cover it with a clean cover slip. See the figure below.
  4. Using your eyedropper, place a drop of water on the edge of the cover slip where it touches the glass slide. The water should be sucked under the slide if done properly.

Technique for Adding a Stain when making a Wet Mount

  1. Turn on the microscope and place the slide on the stage; making sure the "e" is facing the normal reading position (see the figure above). Using the course focus and low power, move the body tube down until the "e" can be seen clearly. Draw what you see in the space below.

Part III. Determining Total Magnification:

  1. Locate the numbers on the eyepiece and the low power objective and fill in the blanks below.

Eyepiece magnification ______________

(X)

Objective magnification ______________

Total Magnification _____________X

  1. Do the same for the high power objective.

Eyepiece magnification ______________

(X)

Objective magnification ______________

Total Magnification _____________X

PART IV. Conclusion Questions:

1. State 2 procedures which should be used to properly handle a **light microscope.

  1. Explain why the light microscope is also called the compound microscope.
  2. Images observed under the light microscope are reversed and** **inverted. Explain what this means.
  3. Explain why the specimen must be centered in the field of view on low** power before going to high power.

Part VV. Plant and Animal Cells Lab

Objectives:. Students will observe onion cells under a microscope. Students will observe cheek cells under a microscope. Students will draw cells and identify organelles.

Pre lab Questions:

**1. What are cells?

  1. How are cells similar to the bricks of a building?
  2. How are animal cells different from plant cells?
  3. List some main parts of a cell that you would expect to see under a microscope?**

Materials: microscope, two glass slides, iodine stain, methylene blue stain, two cover slips, an onion, and a toothpick

Procedure onion cells

1. Peel a translucent piece of tissue from the onion. (The smaller the piece the better.) Translucent **means that you can see light through the specimen, but it is not transparent.

  1. Place the piece of onion on a glass slide and add a drop or two of the iodine solution. Cover the** **slide with a cover slip using your best wet-mount making techniques.
  2. Observe the onion cell under both low and high power. Make a drawing of one onion cell, labeling** all of its parts as you observe them. (At minimum you should observe the nucleus, cell wall, and **cytoplasm.)
  3. Draw and label the onion cell at both low and high power:**

Procedure: Skin cells – each person will make their own slide

  1. To view skin cells, first wash hands with soap and water thoroughly, dry hands.
  2. Put a 1 inch piece of clear tape on the top of your hand and rub thoroughly
  3. Remove tape and place face (sticky-side) up on a slide
  4. Add a drop of iodine and cover with a cover slip.
  5. Observe the skin cells under both low and high power of your microscope.
  6. Draw a diagram of one cheek cell and label its parts. At a minimum you should observe the cell membrane, nucleus and cytoplasm.