Lab 5: Quantum Dots-Report Questions | PHYS 598, Lab Reports of Physics

Material Type: Lab; Class: Elastic Waves; Subject: Physics; University: University of Illinois - Urbana-Champaign; Term: Unknown 1989;

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Physics 598OS Optical Spectroscopy (Fall 06) Clegg/Chao/Liu
Lab 5: Quantum Dots – Report Questions
Answer the following questions for your lab writeup (this is in lieu of the questions in the
original lab handouts). Experiment I: Qdot Blinking
Data Analysis
The time-consuming part of the data analysis has already been done. The figure below
demonstrates how this works. The measured intensity vs time for a single q-dot is shown by the
fluctuating data points. You see repeated transitions from a high intensity to a low intensity and
back. The high intensity corresponds to a bright state when the q-dot is emitting photons. The
low intensity corresponds to a dark state when the q-dot does not emit photons (the non-zero
value is due to background).
Analysis of the time trace proceeds as follows. First, a threshold value of the intensity is selected
for each trace. This is shown by the dotted, horizontal line in the figure. For intensities greater
than this, the q-dot is considered on (bright) and for intensities below the threshold, it is
considered off (dark). The intensity data is then digitized to values of 0 and 1 based on this
criteria. This is shown by the thick black lines above and below the time trace. Finally, to
determine the lifetimes, the average values of the on-time and off-time for each trace are
calculated. This is analogous to determining the average length of the black lines at 1.0 to get τon
and the black lines at 0.0 to get τoff.
0.0
0.2
0.4
0.6
0.8
1.0
1.2
020
Measured Intensity Digitized Data
threshold
value
ON
(bright)
OFF
(dark)
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Lab 5: Quantum Dots – Report Questions

Answer the following questions for your lab writeup (this is in lieu of the questions in the original lab handouts).

Experiment I: Qdot Blinking

Data Analysis

The time-consuming part of the data analysis has already been done. The figure below demonstrates how this works. The measured intensity vs time for a single q-dot is shown by the fluctuating data points. You see repeated transitions from a high intensity to a low intensity and back. The high intensity corresponds to a bright state when the q-dot is emitting photons. The low intensity corresponds to a dark state when the q-dot does not emit photons (the non-zero value is due to background).

Analysis of the time trace proceeds as follows. First, a threshold value of the intensity is selected for each trace. This is shown by the dotted, horizontal line in the figure. For intensities greater than this, the q-dot is considered on (bright) and for intensities below the threshold, it is considered off (dark). The intensity data is then digitized to values of 0 and 1 based on this criteria. This is shown by the thick black lines above and below the time trace. Finally, to determine the lifetimes, the average values of the on-time and off-time for each trace are calculated. This is analogous to determining the average length of the black lines at 1.0 to get τon and the black lines at 0.0 to get τoff.

0.

0.

0.

0.

0.

1.

1.

0 20

Measured Intensity Digitized Data

threshold value

ON

(bright)

OFF

(dark)

The analysis was done for a series of time traces at each power (low, medium, and high). You will find the results tabulated in the MS-Excel files TauPlots##.xls within each data set. The value of τon represents the average time spent in the bright state and τoff the average time in the dark state. The data files ##tr ###.dat contain the intensity vs time data. They are listed in 3 columns – the first column is the time in seconds, the second column is the q-dot intensity, and the third column is the background intensity (you only need the first two columns).

Report Questions

Include the following items in your report for this experiment:

  1. Prepare a plot of the two time traces included for each power (6 traces total). If you plot more than one trace on a single graph, offset the values so they do not overlap. Label each trace as low, medium, or high power and include the average values of τon and τoff from the Excel files.
  2. Question: In terms of the Auger ionization process discussed during the lecture: Why does τon decrease with increasing power? Why is τoff relatively constant as a function of the power?
  3. Question: Based on the calculated lifetimes for each power and assuming fluorescence efficiencies of 0.8 for the bright state and 0.0 for the dark state, what would be the expected quantum yield of fluorescence for the ensemble population?
  4. Question: You saw that when beta-mercaptoethanol (BME) was added to the solution, the rate of blinking decreased significantly. Give a reasonable explanation for how BME is able to suppress blinking. BME has the chemical structure HO-CH 2 -CH 3 -SH.

Experiment II: Fluorophore Photolysis

For each lab section, data for each dye has been posted. You are responsible for analyzing data for all three dyes (fluorescein, Cy5, and Q-Dot 655). You can download the data as ascii files for the lab section you attended.

A. Fluorescein and Cy5.

Using the intensity values that you downloaded, fit the data to the equation given in the write-up. While you can use the approach given in the writeup, it may be easier to fit to the exponential form with fd and τpb, the start time to and initial intensity Iof as adjustable parameters for the fit.

− = − − − d pb

o f f d f

t t I B I B f

( ) (^0 )( 1 ) exp

Here B is the background intensity (without the fluorophore ) and is provided in the header of the ascii files. Some groups did not provide a background measurement – in that case, also make it a fit parameter.