


Study with the several resources on Docsity
Earn points by helping other students or get them with a premium plan
Prepare for your exams
Study with the several resources on Docsity
Earn points to download
Earn points by helping other students or get them with a premium plan
Mastering Microbiology Chapter 6.
Typology: Exams
1 / 4
This page cannot be seen from the preview
Don't miss anything!



In a microbiology laboratory, what does culturing mean? - ANSWERCulturing involves growing microbes in the lab What is the purpose of using the streak plate method? - ANSWERStreak plates lead a scientist toward a pure culture for identification or examination of a single bacterial strain The goal of streaking for isolation is to spread an inoculum of bacteria thinner and thinner with each pass for the identification of individual colony-forming units. What action must be performed between each step to ensure this? - ANSWERSterilization of the loop must be performed Partially digested yeast, which has varying chemical elements, is added to a medium that has been created with exactly measured ingredients. What type of media has been produced? - ANSWERcomplex media A medium designed to change color in order to indicate growth of certain types of bacteria would be considered a __________ - ANSWERdifferential medium Why might a hospital choose to use transport media to carry clinical specimens? - ANSWERTransport media are designed to keep microbes stable until they can be inoculated into appropriate growth media Because some target bacterial species are out-competed by others during growth on media, it is sometimes necessary to inhibit the growth of the
competitors. What type of medium is specialized for this scenario? - ANSWERselective media What is a colony-forming unit? - ANSWERone or more cells that give rise to an assemblage of identical cells You are working in a research lab trying to determine which mixtures of bacterial species can form biofilms. To determine this, you perform a bacterial growth study looking at the number of viable bacteria cells remaining after treatment with penicillin (an antibiotic). You know that all your mixtures of bacteria are susceptible to penicillin when they do not grow as a biofilm. You include a control mixture in your study that you know does not form biofilms. Three different experimental mixtures (Groups 1, 2, and 3) and your control were grown at the same density of cells (12,000 cells per dish). You treat all groups with the same dose of penicillin for 10 hours. You count the number of viable cells 6 hours and 48 hours after the treatment ends. The graph below shows the data from this experiment. Which of the bacterial mixtures(s) grow as a biofilm? - ANSWERGroup 2 What results when a single bacterium reproduces? - ANSWERTwo genetically identical daughter cells If you begin with six cells, how many cells would you have after three rounds of division? - ANSWERForty-eight cells Which of the following is NOT a step in bacterial cell division? - ANSWERDisappearance of nuclear envelope
A pure culture is composed of cells that arise from a single (cell/inoculum/sample) - ANSWERcell Microbes in the __________ phase of the microbial growth curve are most susceptible to antimicrobial drugs - ANSWERlog The ________ of a population is the time it takes for the cells to double in number - ANSWERgeneration time Label the parts of a typical microbial growth curve for bacteria inoculated into a closed vessel of liquid medium - ANSWER What does halophilic mean? - ANSWERIt means the organism is "salt loving". What does the term facultative anaerobe mean? - ANSWERFacultative anaerobes can grow with or without oxygen. More growth is evident when oxygen is present. What does the term mesophile mean? - ANSWERMesophiles grow well at body temperature. Which organism is most likely to be responsible for the boils? - ANSWERStaphylococcus aureus What is a boil? - ANSWERA boil is a red lump in the skin that may be warm and painful to the touch. It is a localized accumulation of pus and tissue debris.