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An overview of the functions of cell membranes, including their role as a selective barrier and the various ways substances can cross them. Topics covered include diffusion, osmosis, facilitated diffusion, active transport, and endocytosis. Real-life examples are given to illustrate the concepts.
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Chapter 3
Membranes surround the outside of the cell and the organelles inside it. The plasma membrane acts as a selective gatekeeper. A substance may cross the membrane: By diffusion By a mediated transport system By endocytosis
Cell membranes are selectively permeable – water can pass through, but not most solutes. Gases (oxygen & carbon dioxide), urea, lipid soluble solutes can cross the membrane.
Osmosis - if there is a membrane between two solutions with unequal concentration of solutes that can not cross the membrane, water will flow toward the side with less water / more solute until the two sides have equal concentrations.
Animals utilize osmosis to control internal fluid and solute levels. The blood of marine fishes has 1/3 the salt content of the water. They are hypoosmotic to seawater. Freshwater fishes have blood that is saltier than the water. They are hyperosmotic to the water. If the solute concentrations were the same, the two solutions would be isoosmotic.
Charged substances, like water and dissolved ions, can’t simply diffuse across the cell membrane. They pass through channels created by transmembrane proteins. Some channels always open. Some are gated channels.
Sugars & amino acids must be able to enter cells and waste products must be able to leave. These molecules cross the membrane with the help of transporter proteins. Transporter proteins are specific. Facilitated diffusion Active transport
In facilitated diffusion , the transporter protein binds to the substrate molecule on one side of the plasma membrane then changes shape to release it on the other side. Takes place in the direction of the concentration gradient.
Endocytosis is the ingestion of material by cells. Phagocytosis – cell eating – method of feeding by single- celled organisms. Pinocytosis – small molecules or ions are enclosed in vesicles called caveolae. Receptor-mediated endocytosis – method of bringing large molecules into a cell with the help of the protein clathrin.
Exocytosis - membranes of a vesicle inside the cell can fuse with the plasma membrane to discharge the contents of the vesicle outside the cell. Transcytosis – a substance may be picked up on one side of the cell, transported completely across the cell and discharged on the other side.