Portage BIOD 171 M3 Exam Latest Update 2023
Nanometer ( definition) - answers 10-9 or one-billionth of a meter
Micrometer (definition) - answers 10-6 or one-millionth of a meter
2 critical factors that influence your ability to see an object - answers Resolution and contrast
Difference between resolution and contrast - answers resolution refers to the distance between two or
more objects at which they can still be seen as separate. contrast is the difference in light absorbance
between two or more objects.
if you increase the light going into a microscope, what part would you adjust? - answers the iris
diaphragm
as light passes through a microscope, what is the last piece that light passes before reaching your eyes? -
answers ocular lens (eyepiece)
How is total magnification calculated? - answers Multiply the ocular lens (10x) by the objective lens
one limitation to fixing a sample? - answers fixation requires you to kill your sample. this makes
determining motility of a sample impossible. Fixation can also distort the specimen shape and
arrangement
The benefit of phase-contrast microscopy is that it: - answers it provides detailed image of live cells
without staining
Distinguishing Features of dark field microscopy - answers exceptionally dark background and vibrant
specimen
fluorescent microscopes illuminate samples through what spectrum? - answers ultraviolet sprectrum
