Docsity
Docsity
Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity

Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan

Guidelines and tips
Guidelines and tips
Sell on Docsity
Sell on Docsity
Docsity AI
Docsity AI
Log inSign up

Prepare for your exams

Study with the several resources on Docsity

Find documents

Prepare for your exams with the study notes shared by other students like you on Docsity

Search for your university

Find the specific documents for your university's exams

Docsity AINEW

Summarize your documents, ask them questions, convert them into quizzes and concept maps

Explore questions

Clear up your doubts by reading the answers to questions asked by your fellow students

Earn points to download

Earn points by helping other students or get them with a premium plan

Share documents
download point icon20 Points

For each uploaded document

Answer questions
download point icon5 Points

For each given answer (max 1 per day)

All the ways to get free points
Get points immediately

Choose a premium plan with all the points you need

Study Opportunities

Choose your next study program

Get in touch with the best universities in the world. Search through thousands of universities and official partners

Community

Ask the community

Ask the community for help and clear up your study doubts

Free resources

Our save-the-student-ebooks!

Download our free guides on studying techniques, anxiety management strategies, and thesis advice from Docsity tutors

Microscopy Techniques and Staining Methods, Exams of Nursing

Daystar UniversityNursing

A comprehensive overview of various microscopy techniques and staining methods used in biological and medical research. It covers topics such as the definition of a nanometer, the differences between resolution and contrast, the components and functions of a microscope, the principles and applications of different microscopy techniques (e.g., bright field, phase contrast, dark field, fluorescence, confocal), and the use of staining techniques (e.g., gram staining, giemsa staining) for the identification and visualization of microorganisms. The document also includes practice questions and answers to test the reader's understanding of the concepts presented. This resource would be valuable for students, researchers, and professionals in the fields of biology, microbiology, and medical diagnostics who need to understand and apply microscopy and staining techniques in their work.

Typology: Exams

2023/2024

Available from 08/22/2024

wil-mug
wil-mug 🇰🇪

4.2

(5)

2.3K documents

1 / 6

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
BIOD 171 MODULE 3 EXAM QUESTIONS WITH
CORRECT VERIFIED ANSWERS/ ALL MADE TO
BOOST AND EASE YOUR STUDY/ RATED A+ /
PROFESSOR VERIFIED
A nanometer is defined as: - ANSWER 10 -9 or one-billionth of a meter
True or False: A nanometer is longer than a micrometer - ANSWER False
A nanometer is 1000x smaller than a micrometer
Resolution and contrast are two critical factors that influence your ability to see an object. Explain each. -
ANSWER Resolution refers to the distance between two objects at which the objects can still be seen as
separate. Poor or low resolution means two or more objects may appear as one.
Contrast on the other hand is the difference in light absorbance between two objects. Poor contrast
gives a high background and makes the visualization of multiple objects difficult. For instance, trying to
identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the middle of
a sunny afternoon (bright light against 2 dark objects = high contrast).
Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance the
magnification of a sample. - ANSWER Objective
Only the oculars (eyepiece) and the objectives contribute to the magnification of the sample. Since the
eyepiece
is fixed, only the objectives could be altered.
pf3
pf4
pf5

Partial preview of the text

Download Microscopy Techniques and Staining Methods and more Exams Nursing in PDF only on Docsity!

BIOD 171 MODULE 3 EXAM QUESTIONS WITH

CORRECT VERIFIED ANSWERS/ ALL MADE TO

BOOST AND EASE YOUR STUDY/ RATED A+ /

PROFESSOR VERIFIED

A nanometer is defined as: - ANSWER 10 - 9 or one-billionth of a meter True or False: A nanometer is longer than a micrometer - ANSWER False A nanometer is 1000x smaller than a micrometer Resolution and contrast are two critical factors that influence your ability to see an object. Explain each. - ANSWER Resolution refers to the distance between two objects at which the objects can still be seen as separate. Poor or low resolution means two or more objects may appear as one. Contrast on the other hand is the difference in light absorbance between two objects. Poor contrast gives a high background and makes the visualization of multiple objects difficult. For instance, trying to identify 2 dark colored objects at night (low light = low contrast) versus the same 2 objects in the middle of a sunny afternoon (bright light against 2 dark objects = high contrast). Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance the magnification of a sample. - ANSWER Objective Only the oculars (eyepiece) and the objectives contribute to the magnification of the sample. Since the eyepiece is fixed, only the objectives could be altered.

Assuming a constant (non-adjustable) light source power, identify the part of the microscope you would adjust to limit the amount of light entering the microscope. - ANSWER Iris diaphragm What is the total magnification on (relative to your eye) of a sample imaged with a 60x objective and a 10x eyepiece? Show your math. - ANSWER 60x objective x 10x ocular = 600x magnification True or False: Staining is often required to image a cell that is adherent and flat (thin). - ANSWER True Adherent, flat and unstained cells are almost invisible due to the limits on both resolution and contrast. Therefore, cell staining is often required to adequately image the sample. True or False: A cell that is adherent, flat (thin), and unstained is easily identified using bright field microscopy - ANSWER False: Adherent, flat cells are almost invisible due to the limits on both resolution and contrast Which of the following could be seen clearly by the unaided eye? Select all that apply. A. Bacteria with diameter of 24 μm B. Protozoa with diameter of 150 μm C. Virus with a diameter of 0.2 μm D. Skin cell with diameter of 1500 μm - ANSWER B. Protozoa with diameter of 150 μm D. Skin cell with diameter of 1500 μm The unaided eye can, on average, clearly resolve objects > 100 μm

  1. Which of the following could NOT be seen clearly by the unaided eye? Select all that apply. A. Bacteria with diameter of 24 μm

This type of microscope is capable of capturing images in multiple focal planes, rendering a specimen in 3 - D - ANSWER Confocal Identify what type of electron microscope was used to capture the following image and explain your choice. (picture labeled B) - ANSWER The image was captured using a Scanning Electron Microscope (SEM). The above image shows the trademark 'shell' image (no subcellular organelles are visible) reminiscent of SEM. Only TEM is capable of visualizing subcellular substrucutres. Identify what type of electron microscope was used to capture the following image and explain your choice. (picture labeled A) - ANSWER The above image is captured via a Transmission Electron Microscope (TEM). Even at 20nm resolution (inset image), subcellular substructures are still visible. The image lacks the outside 'shell' only appearance of SEM. Gram-Positive cells appear [answer1] in color due to a [answer2] peptidoglycan layer in the cell wall. - ANSWER 1. Purple

  1. Thick Gram-Negative cells appear [answer1] in color due to a [answer2] peptidoglycan layer in the cell wall. - ANSWER 1. Pink
  2. Thin True or False: A Giemsa stain can be used to determine the presence of pathogenic bacteria. - ANSWER True Pathogenic bacteria would appear pink while non-pathogenic bacteria would appear purple. True or False: The distinguishing characteristic of Gram-Negative bacteria is the presence of LPS in the outer membrane. - ANSWER True True or False: Following the decolorization step of the Gram stain, Gram-Negative bacteria will appear colorless. - ANSWER True

True or False: If you wish to study the motiity of an organism you cannot heat fix, but you can chemically fix the specimen - ANSWER False Both heat and chemical fixation strategies will kill the cell, making motility observation impossible Name one substance capable of chemically fixing cells to a slide. - ANSWER Any of the following are true: Paraformaldehyde, ethanol or methanol. You suspect a patient may have TB. Once a sample has been obtained, it is sent off to the lab for an acid- fast stain. If the patient were infected with TB, describe what you would expect to see on the stained slide. - ANSWER I would expect to see red cells (TB+) on a blue background (TB negative). You want to observe the size and shape of a cell. What is the easiest staining technique that you could perform? Name at least one dye you would use during this process. - ANSWER Simple stainining and crystal violet could be used. True or False: If a patient is suspected of having malaria, a Giemsa stain would be an appropriate differential test to perform. - ANSWER True Giesma stains are often used in the clinical setting aid in the diagnosis of blood parasites. A human blood sample from a patient with a suspected parasite needs to be stained for diagnosis. Which stain should be selected? - ANSWER Giemsa Select the type of microscopy used to generate the image: (black background with green glowing cells) - ANSWER Florescence microscopy You are the lead technician in the Microbiology Department's core Microscopy lab. You are given a list what different scientists would like to observe in their samples. You must match these samples with appropriate type of microscopy.