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MLS ASCP Recall, Exams of Nursing

University of CambridgeNursing

A series of multiple-choice questions and answers related to various topics in medical laboratory science, including primidone monitoring, pure red cell aplasia, identification of cryptococcus neoformans, drug toxicity screening, infectious mononucleosis, reactive monocytosis, sporothrix schenckii, carbon dioxide measurement, antihuman globulin testing, acid-fast bacilli staining, 5-hiaa in urine, statistical analysis, auramine-rhodamine staining, streptococcal pharyngitis and glomerulonephritis, cerebrospinal fluid specimen handling, paroxysmal nocturnal hemoglobinuria, and anti-p1 antibody neutralization. A review or practice material for medical laboratory science professionals, covering a wide range of topics relevant to their field.

Typology: Exams

2023/2024

Available from 07/27/2024

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MLS ASCP Recall
Primidone monitoring: suspected to have primidone overdose, but the serum
concentration is within the normal range, what should the MLS do next?
Measure procainamide concentration
Measure phenobarbital concentration
Measure phenytoin concentration
Eliminate primidone overdose as possible diagnosis - Answer B. Measure phenobarbital
concentration
Feedback Primidone is a structural analog of phenobarbital.
2-month old baby with Normal WBC, Normal platelet, reticulocyte count: 0.1%:
Pure red cell aplasia
Chronic Myelogenous Leukemia
Myelofibrosis with myeloid metaplasia
Polycythemia vera - Answer A. Pure red cell aplasia
Feedback In pure red cell aplasia only red cell production is affected and WBC and
platelet counts are normal. It is not likely that RBC count falls that low in lead poisoning.
Which of the following stain is recommended for identification of Cryptococcus
neoformans?
Methyl red
Congo red
Wright stain
India ink - Answer D. India ink
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MLS ASCP Recall

Primidone monitoring: suspected to have primidone overdose, but the serum concentration is within the normal range, what should the MLS do next? Measure procainamide concentration Measure phenobarbital concentration Measure phenytoin concentration Eliminate primidone overdose as possible diagnosis - Answer B. Measure phenobarbital concentration Feedback Primidone is a structural analog of phenobarbital. 2-month old baby with Normal WBC, Normal platelet, reticulocyte count: 0.1%: Pure red cell aplasia Chronic Myelogenous Leukemia Myelofibrosis with myeloid metaplasia Polycythemia vera - Answer A. Pure red cell aplasia Feedback In pure red cell aplasia only red cell production is affected and WBC and platelet counts are normal. It is not likely that RBC count falls that low in lead poisoning. Which of the following stain is recommended for identification of Cryptococcus neoformans? Methyl red Congo red Wright stain India ink - Answer D. India ink

Feedback Cryptococcus neoformans, because of its large polysaccharide capsule, can be visualized by the India Ink stain. Organisms that possess a polysaccharide capsule exhibit a halo around the cell against the black background created by the India Ink. Drug toxicity screening of a 6 year old girl has increased presence of ACETAMINOPHEN. What test should be done next? ALT and AST BUN and Creatinine Salicylates CK and LDH - Answer A. ALT and AST Feedback Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations begin to rise within 24 hours after an acute ingestion and peak at about 72 hours. In severe overdose, transaminase elevation can be detected as early as 12- 16 hours post-ingestion. Toxicity is defined as serum AST or ALT concentrations greater than 1000 IU/L. A rapid progression of transaminase values to 3000 IU/L or greater reflects severe hepatotoxicity. Causative agent of Infectious Mononucleosis: Cytomegalovirus Epstein-Barr virus Poxvirus HPV - Answer B. Epstein Barr Virus Feedback Epstein-Barr virus (EBV), also known as human herpesvirus 4, is a member of the herpes virus family. It is one of the most common human viruses. EBV is found all over the world. Most people get infected with EBV at some point in their lives. EBV spreads most commonly through bodily fluids, primarily saliva. EBV can cause infectious mononucleosis, also called mono, and other illnesses. Reactive monocytosis is seen in: Tuberculosis Allergic reaction Hypersensitivity

Feedback Check cells are used as a quality control measure for negative indirect or direct antiglobulin tests performed in test tubes and should always be positive when added. A positive culture of sputum was stained. Carbolfucshin was added, washed, decolorized and malachite blue was used as counter stain. Two entire field was scanned and no acid fast bacilli were found. The most probably cause is: Wrong primary stain was used Wrong counter stain was used Inadequate scanning of slide Inadequate decolorization - Answer C. Inadequate scanning of slide Feedback Good practice in reading AFB slide should be 100 fields. 5HIAA in urine indicates: Carcinoid tumors Renal disease Nephrotic syndrome AOTA - Answer A. Carcinoid tumors Feedback The 5-hydroxyindoleacetic acid (5-HIAA) urine test is used to help diagnose and monitor carcinoid tumors. It may be ordered by itself or along with a blood serotonin and/or chromogranin A level. 5-HIAA is the primary metabolite of serotonin that is excreted in the urine. Concentrations of 5-HIAA may be significantly increased when a person has a carcinoid tumor that produces serotonin. Which of the following is used to compare two sets of mean? Chi-square CV Standard Deviation Paired T-test - Answer D. Paired t test Feedback Paired T-test-Calculate a range of values that is likely to include the population mean of the differences

The purpose of POTASSIUM PERMANGANATE in Auramine-Rhodamine stain is: QUENCHING AGENT True False - Answer A. True A patient was diagnosed with streptococcal pharyngitis 6 months ago. Now he is showing signs of glumerulonephritis. What will be seen in the renal biopsy? S. agalactiae S. pyogenes H. influenzae S. aureus - Answer S. pyogenes Feedback S. pyogenes is the most common causative agent of pharyngitis. A CSF specimen was sent to the laboratory at 2am, gram stain was done and subsequent culture is to be done by the next shift. What temperature should be the specimen kept? Incubated at 35 degrees celsius Stored at room temperature Stored at 70 degrees celsius Freeze the specimen - Answer A. Incubated at 35 degrees celsius Feedback Fastidous organisms such as Neisseria and Haemophilus frequently isolated from the CSF of patients with bacterial meningitis are preserved by placing the fluid in 3%-7% CO2 at 35C-37C (or at room temperature for no longer than 30 min), if the specimen cannot be cultured immediately. Defect in PNH (Paroxysmal Nocturnal Hemoglobinuria)? Spectrin CD55 and CD CD