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MMSC 407 Exam 1 with Complete Solution
1.
Spectrophotometry:
measurement of the absorption of electromagnetic radiation of a substance at
ditterent wavelengths of the spectrum
2. tungsten light: visible light
3. hydrogen/deuterium light: UV light
4. xenon/mercury vapor light: UV light
5.
Monochromator:
a device that will select a narrow range of wavelengths of light from the continuous
spectrum that is provided by the light source
6.
Interference
Filters:
composed of 2 parallel sheets of half-silvered glass surrounding a core of MgF2; gets dirty easily
7.
Prism:
narrow band pass; expensive
8.
diffraction
grating:
composed of a polished reflective surface onto which sharply defined, parallel groves
have been etched; narrow band pass; best
resolution; expensive
9.
cuvette
shapes:
round,
square
10.
type
of
material:
clear glass, quartz, plastic
11.
Photomultiplier
Tube
(PMT):
amplifies the primary signal; best and most sensitive photodetector
12.
Photo
spectrometer
quality
control:
known-unknowns ran as patient samples with a known
analyte concentration
13.
Nephelometry:
measures
light
scattered
14.
Turbidimetry:
a
measure
of
amount
of
light
transmitted
through
sample
15.
scattering:
redirection of radiation out of original direction of propagation, usually due to interaction with
molecules and particles
16. what method is more sensitive (nephelometry or turbidity)?:
nephelometry
