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SG VID 24 PuZOL Observation. of cheek Cet By vi tod dtataing. Re Sull and observation . ~ — ’ The cheek cells were obSewed | ~ Choek cells haven't a defnite 2 > the. vetlel ene! Starred Gh Ure blue Gnd the Muclercs wes blue > the epidermal. | ces have deffnite. shape ble op ceu | | wocelt ~> the wutleas af epidermal. colk. unr cloax- | Diswwsion : | Nature “ap cheek cebu : g > Viman buceal sikelfal tea ae enary off Cs | aparnour cells fackino, q. calh Walls roluich ais nqurshes | then from Plank ColLs => owe. a ie ulav shape and are teasely ovanged ue Ut ‘the. dosence oF gtru cluxal mar rae by provided Ae qa cell wall iene TS Beans paxank) making the pnstafnol ceils me eb Suje to obsewe. under | a Nucrescope ~ Roles methyl: one pluie stating » Rue we a Caltonie. (pothvelg inds to ees change | aids CDNA and ene | ikea. appeant | of fis st 7 , Amin ] ———— 7 Statin he bs | aud ulna allowing Morphology and Shou cloeal | | | 3. we 0-4 T- wael or ilps dePhonenMate polweor for the steady of we fo wu leas © cell osganitation . t cab saline : jolog anks osme re > the Isokonte saline dolulton prev Lysis or dehy dation of cells , main tat Nolwtal jor phe logy. ning thas © not giank 6% > this ensures that the cols a0 ° | 9) Swe preaewing thet atiwrabe —mbcr oscopie Aout. frbagn oxaminalion - ay a mountin ly Per yeduun 4 of jt Addiffon. contin | ~> billy centre. vewents the | ond acks ab & got rach ve. Improving clazui by ander > tt also provi dis paevrting movements qa sew — peu of. the Soup le celbs from daying out indox— makchir4 meatom, the- Mi crostope ‘ anent mount s and allowing. Lor prolong? ‘pbs evvalton - 6. wek mount praporation and lovers lip App tcalfon > A wot mount is psd Ae cbse We ; 7 | | 5 puffed tthe wo 2. Fractionation of Fok, axqor'c cell Rosutt : The Cellular conponaitls of an pu ofc eeu A dows Fy Lhd | aaaled based oh gize mH Wo sep AitPoron hal corel fuagatt on. Disuusysron- | 7? wed ho ssolate brlfexonk pagal? . SO Tha Cay be bludied [i 5 this enabler ¢ndivi dual ongauce Staub Lyyhors bo be bludied - | > cells VO boon open 4a sabease The conlenls , caked wd 0 ano. Thon dep wud in a bold , isakowt c aul “ate tats must DE PMP i buffered Solukion - | oy mid: to moduce, CUTE artivity , when the cod : a we gobeas which could pyeaks open mn _sqsobonic * put Ae the same Water potertiat bo puowert osmosis ae THE would couse the org anole be shvivel bv busat sduilion bar a pil buffer te proven damage 4 onqoneles What ¢ . boog! sm | | epost 2 000g | 20m chiov p’ an Sapearaboal | lomogind 2 ¢ Vv | 0, 0009 70m" 1, oF 4 poy witb Say ao ah seu v0 jmifochorduia rae eurat ard Ry fos] Sup * \ | ——~ (ute) [o,00 J] 2hos (S,e0yR > friea Jomin ’ yibo Somes / and micosomos Supounadan V/ Tf tus Sup enataal ; Nove Qenbytfe od for Prolonged peuieds al foxces a i le 400) 000 4, ths di ssol vel probein ; nucleic auds ,Splub! ¢ try would Subron) a 7 Dang effets : vane Wao Fmpart of. candidate on Xolated oxqanell , uch ansers A) | Melochond ial Foxtel fs vital for preclintcat dog Eosk ny . ,~? pro teomic. profilin : Pselahna dpesiffc org ON Atlnws Lpr far chek arokeomic- analysts , \ tein dent Fealio n blow kos saw finds on ite pre Satenackons jtthin ov ayrell es . k A ' + ps gallos => Disease metharusms *» relation of Spex c ev enbrahve em diseased ceels Ce-g-y puijo chondsua yor neunroasg 4 disper dens) ads n understanding disease Pathogeres!s an Vda fying angels for thoy © ] 1 | = BBR epee, i 7 eS sf = [in WW d aity Len ak emake ™ : ; bensrtd | 6 petit 7 =] pK yrediem 0 medium \-" Tastky particles Lange Or lugh aoes'ty particles | | esr ne Sa 1 cer “fagakion nn | TT | | 3 plasmolysis al Hemolysis | Result. the blood was hemolysed. \ Discussson - «tha Tup lw. of nad Hood calls (RBC ») Han ol BIS, ard He Subsequent xotoase of hernog lo the plasma ,fs a hoy piolog ical proces with St Ymplicakions sn both ovat physiology and patrelog y re u - See Low concent ah on TL the cell Conus eee F = 7 - ~ \ ! ty pad Fr conta with “7 . * a 20" ball pnernvons Water a Serpe y CO GOS” Corte cos Zeme atkag Selub'on = eg 2 . : Potnod ¢ Whew ocmol’é —, — mombiane) p huey vf CeU presuse ts : Sw ething ‘and Rit lesa than Tee of (ath dap present: oun tha (ek Wale . Ths Canbes Suing or sup bare of. The Cou Tontoty - he ability gf 0” erdra cellular Solubion Ae (aus & te yuove monk of Solvent yleules Into oO put of a Cells A dolulion pith Faee dolube. toncentyalioa \ { Tetonic silelon ee | gs thot Frwn witun aC lig po toni dolulton: A Solutton wrth lowest golut 2 Con centrabi on tha thak foun poi tun a tet - Hy per tontc Solult on: A Solution pith ha hen solute hion Fan that found wiltn oe ce | ot. different tonic Solution of colt fuvg lily his step provents Jronwoly sts —~—_. A-A _ pemonstva bon ¢ cel pivision By milosts Result - . The experiorent succemfully in onton oot Kp cella Nea. prepare Rp slae y beth Aitter phase and prophase cols obs ened qlexphase wal tl pwtlro as the prom Phase _ while pophas was obsousred through Chromosomal lon ensalfon aad nutleart mombyan€ . The. clear arfherenHation Lelwoeen interphase | hig his fre Shuckuxal changes that | ‘tal flag e- of mitohic Livisten dom onstrated mitosis d bntor yoot pow inenk } rf pre aizaov A wai be phase Ora dues ng Ha in Dis wxston : 1. the onion soot Hp tpntuns A fang € number of adivd dividing cally tn teal nevis berm ) MA“G ) an oeok yesen lp sku milolic stg es. ene Walls *- 2- Wydnech ce aad (ud sobering & Hcl breaks down the wi dole. lamella He coll miby & z funthon ° " aud weakens jue etl wall) making nA , faulitares Lett-er chai Chromosomes Proper $Y harng permeable ly stat Wares a4 pcelocasamine 6 tain: Dee Stairs gelechrvely brad lo PVA pad yisib te undan the Chyomalin, waldng he Chromo s om eh Nivasonin’ bs a gerowl ov" dbtacn, th deory ribose. gr In DVA, peutic Chromosomot prswabezakte» micros lope ) Ac Phome of cou cyele | ? Nuclear envelop 2 & ynucleolus ve distinct . forming polw ork yw hun | J D Chromattn frbets appar Nucleus 9 axe not grstingudshed ynte chromosome S- | prophase. | 2 Chromatin material Shorter A condshSe into thread [ice Ahuy tees cabled Chromos orm e% 7 each chromosome eonsisk sp Loe chvoraal'ds soinbod | ok Qe pol nt catled contvonieré - > mb end F prophase. wuclen ws % Musee membrane | dis app emt the (oltulan protein contents songense Inte a opindle- | 3. oka phate. | zh bipolar , prastvat develop s in the Cells. > the chrome sem ep pee ghort 2 frtad ham'ty koo ot sdtahed te me clean KG eto > he chroma a b yor, oF fhapr contrornee> - ed oat the egpalor of a po bY tossing Lutaphase +S 4.6 Demonstration of cell Division . by merosis Result: denvonelvades The the experimen’ Success wets! sin Trades canta pollen poten Cells. Disceess von “nadosts — ©. Credutbional Liston) prophase F- Chromosomes L, Jomolegor Clyomsomey pair Coy sis) ' and cross) ty over otut cbseued ad chiasmada ~whicle purhates gow Vantatuo n , _ = uelaphate TH The lLomblogeus loco rnpsovnn- a | cundow at te yaa gre plate. Slows 4} im Ans ovtwent- [7 A phase. F— omplogous hwmosomes wee potted axpautt | bp oppost Fe poles , ge the clucrmosome humper by | hall > Telaphase + and tubo Kinesis» 1000 haglard daughter cous | poo. formed. , oath tondaviiy Aah the ed cleanest awmber but RIL in duplicated 2m Heipst¢ Jb Cespichonal pivision) prophase. — Chromo somes fondowed age » but no over otuuxed « 5 abig wed dopencent at the toutex of [praphane ae aie Chenabbda wo u Isken chromatids Wen od | rad poved howard opposi te. poles - separa | epee a and tylokinesis > four haplaid Cots wera Poevdut each Cont au a enkt | aera hiot Aun ‘te aos ng overt an J jndeypendenk arysorlment: > Hydrochloric ante Ctlet)~ Softentns Tissuss *. » iN jel byeaks down Hoe Call Walt and soflens lisswes , Making yt easier de exctvatk and Stan PMés >Acctocaxamine Slash _gelectively binds bo PNA aid Chromatin , staining chromosomes yed - helps ideale Hoi okic skages Snaps, crossing OVE , Chiromosome. Segregation - 3 Yo 7 ethanol Crivalive) — presewing collulas | Shuck > fires cotl» by preseuir'y ‘thery Chyoneosomal inbepaily and preventing degradation: te Sproad a shin | yor | to flatten — of Speemen -> wed fo obsewe | > vsed ko odbseure de | PTvidual cue ved) a anpertsam. | — { | ¢ 2 Speumen ws prowed od ai Sate Qorose lesb Che ed covers le | Ae oe a > spetimen ts Axed and P ~? Speem be rod ee) : and ‘Nainsd bebose-on Bee before apply Wy likaay H Freamans (200). Brolog tad. Sulence 7 ). Hobousr 1 NY- Pearson “ p- 2b * jolumel, ® b) oN ‘i of wocl , Bovk p (200 Tnhexacktve Jet Life ™ prchieved from Yo Prigingl on LY ed 23 fuly 201 Soman org - Reb Suieve i 5-A- tolalion ~ Estimation of beenome DNO ~ thicken Liver. — | Result: / Dun was Success tally Foote bod from jhe Chuckon livey I par Jeolalon- —Sceth Lysis ¢ “Ife chicken liven Lisue wv Ironnng erie! dion buffer tonto aris —cl, Now » bh, an exe : FOTAR aiid ps > SPs. wm DUM OE detergent , disrupls / if pid membwnes , leading jo cell ys aad the goles e J tetluler contend including Duk. tt session of pbsewed values - Liv absorbance Readings ‘ . Tho ghsorbarce value, at varnoud wowrebongth (220 nm bo Bo0nm) parovido. Fnsig hts inte The put | wntentration & te isolaked DUA - gna - chickon livey + Codie acetate : | > the absorbance vabue decrease prog vensively | from 220mm fo 210nm, pith minimal randungs ou QL a jon and beyork. jhds pattern degpe™® Tha. presence guid, vith punimal paotein contamination » Ow proteins typically absorb Sirongly ak 2k nm -» chicken liver + Ua20H '. 7 Higher absorbance values across al noweloneth ntoeatiaatio n 04 incom p let 2 ! ot 2bonm and of. both ruler’ aud oud prmieins a Payh “A thickets on hi Lith d. Li Ll L saber eee tee tame als a 4 ii SIT? aI tp ELSE CES Sete GW - t 3 i me ioe eee Sees pop d-E4 a T Seer tens tt eta aaaal fa Waddell lt Bedd is 4OMF 4, y- AAs, 10K © Ort orale rs, ton nN riclhod wort f prin by pp a, torn o I TF slrmna ered Ida me { eab eet Maes tees aes eset eeepc wivong po Fj isuog | 20 EP SES 
