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Lecture notes from ece 510 spring 2008, focusing on nanotechnology and biosensors in microfluidics. The notes cover topics such as nanotechnology applications, microanalysis, design opportunities, goal of µtas systems, chain of operations, micromixers, microchemical reactors, detection, and detection strategies. The document also includes information on capillary electrophoresis, linear to radial ce arrays, optical tweezers, and hydrogels.
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ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
http://www.ece.pdx.edu/~prasads
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
1. Introduction to Bio-MEMS and Nanobiotechnology2. Silicon Microfabrication3. “Soft” Fabrication Techniques4.Polymer Materials and Microfluidics5. Sensor Principles6.Detection and Measurement Methods7. Drug delivery systems 8.
Micro-Total
Analysis
Systems
( μμμμ
Emerging
Applications
9. Nanotechnology Applications
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
-^
Example:
dia, 1 cm in length ) has a samplecomprising of 2 components of molecularweights 256 KDa and 584 KDarespectively with zeta potentials of –250mV and –290 mV respectively. How canthe two components be separated? Whichwill separate first?
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
Micro fluidics components
Spotting and analyte detection on solid surfaces
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
-^
-^
miniaturized pumps
immobilization
chambers, electrodes and detectors into singledevices
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
Sample introduction and electrophoreticseparation are accomplished in each ofthe two crossing channelsThe sample is driven through the shortsample channel across the separationchannel by application of a potentialThe “plug” is electrophoreticallyseparated by the application of anotherpotential
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
-^
-^
ECE 510
S. Prasad
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
ECE 510
S. Prasad
Slide:
Courtesy: S.S. Saliterman
Lecture 16- Spring 2008
Capillary isoelectric focusing (cIEF) uses a pH gradientfor
isolation.
Different
peptides
and
proteins
exhibit
different
isoelectric
points
that
may
be
used
for
separation
-^
In cIEF the capillary is filled with a mixture of analytes,whereby the ends are immersed in an acidic buffer and abasic buffer
-^
Upon application of an electric field, a pH gradient isformed in the capillary and analyte molecules migrate tothe position within the gradient, where the pH equalstheir isoelectric point, thus they lose their net charge.
-^
Typically, the focused zones are mobilized for detection.
