!
Course!Number!&!Title!
BE518!!!Optical!Microscopy!
Credit!Units!
1"CU"(3"semester"hours)"
Class/Laboratory!Schedule!
Lecture:"3"hrs/week.""Recitation"1"hr/wk."
Instructor!
Christopher"Fang>Yen,"PhD,"Assistant"Professor"of"Bioengineering"
Prerequisites!
Math"240"or"ENM"510"or"equivalent,"or"permission"of"the"instructor"
Course!Satisfies!
(check!only!one)!
[""""]"Math"
[""""]"Sciences"
["X"]"Engineering"
["X"]"Technical"Elective"
[""""]"TBS"
Text(s)/Required!Materials!
Hecht,"Eugene"(2001)."Optics,(4th(Edition."Addison"Wesley,"Course"materials"
Catalog!Description!
This"course"discusses"the"fundamental"concepts"of"optics"and"microscopy,"with"emphasis"on"applications"in"
biomedical"imaging.""Lectures"will"be"supplemented"by"lab"experiments.""We"will"survey"advanced"modern"
techniques"including"confocal,"multiphoton,"and"superresolution"microscopy."""
Topics!Covered!
Light"and"the"electromagnetic"spectrum.""Geometrical"optics:"optical"rays,"refraction,"reflection,"lenses,"mirrors,"
prisms,"optical"systems,"optical"design,"aberrations."Physical"optics:"waves,"diffraction,"interference,"resolution"
limits,"coherence,"lasers,"Gaussian"beams.""Microscopy"m ethods:"Phase"contrast,"differential"interference"contrast,"
fluorescence"microscopy,"confocal"microscopy,"multiphoton"microsco py,"optical"coherence"tomography.""
Superresolution"techniques:"structured"illumination,"STED,"PALM."
Course!Objectives!and!
Relationship!to!Program!
Education!Objectives!
!
The"goal"of"this"course"is"for"students"to"gain"an"understanding"of"the"principles"of"optics"and"the"application"of"
these"principles"in"microscopy.""Students"will"learn"to"design"and"construct"simple"optical"systems."""
Contribution!towards!
Program!Outcomes!
Multidisciplinary"Ability"–"Med"
Problem"Solving"Approach"–"High"
Problem"Solving"Methods"–"High"
Experimentation"–"Med"
Design"–"Med"
Professional"Orientation">>"Low"
Contribution!towards!
Professional!Component!
75%"Engineering"science"
25%"Engineering"mathematics"
Weekly/Session!Schedule!
Week"1:"Foundations:"Maxwell’s"equations.""The"wave"equation.""L ight"and"the"electromagnetic"spectrum."""
Week"2:"Foundations:""Index"of"refraction.""Reflection"and"transmission.""Po larization."
Week"3:"Geometrical"optics":"Optical"rays,"lenses,"mirrors,"prisms"
Week"4:"Geometrical"optics:""Image"formation"
Week"5:"Geometrical"optics:""Optical"systems,"telescopes,"microscopes,"cam eras,"the"eye"
Week"6:"Geometrical"optics:""Optical"design,"optical"aberrations"
Week"7:"Physical"optics:"Diffraction"
Week"8:"Physical"optics:"interference,"coherence"theory"
Week"9:"Physical"optics:""Fourier"optics,"Abbe"theory"
Week"10:"Physical"optics:""Resolution"limits"in"microscopy"
Week"11:"Microscopy"methods:"Phase"contrast,"differential"interference"contra st"
Week"12:"Microscopy"methods:""Fluorescence"microscopy,"confocal"micro scopy"
Week"13:"Microscopy"methods:""Multiphoton"microscopy,"optical"coherence"tomography"
Week"14:"Microscopy"methods:"Superresolution"methods:"structured"illum ination,"STED,"PALM"
Grading!Details!
30%"Homework"and"lab"assignments"
40%"Midterms"
30%"Final"exam""
Prepared!By/Date!
Christopher"Fang>Yen"/"September"2012"
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